Association between the C3435T single-nucleotide polymorphism of multidrug resistance 1 gene and risk of gastric cancer

Abstract

The multidrug resistance 1 (MDR1) gene encodes P-glycoprotein, which confers resistance to antineoplastic drugs, but also affects the kinetic disposition of certain drugs and carcinogens. The C3435T polymorphism of the MDR1 gene may influence the transport and excretion of carcinogens, increasing the risk of cancer. The aim of this study was to evaluate the association between this polymorphism and the risk of gastric cancer (GC). Ninety-eight patients with non-cardia GC and 203 healthy subjects participated in the study. DNA was extracted from leukocytes and the MDR1 polymorphism was analyzed using PCR-RFLP. Serology was performed by ELISA for the investigation of infection with Helicobacter pylori. No significant difference in the genotype (p=0.668) or allele (p=0.745) frequency of the C3435T polymorphism was observed between the GC and control groups. There was no association between the genotypes studied and the risk of GC in patients infected with H. pylori (p=0.662). Patient survival was not correlated with the genotypes studied (p=0.454). No correlation was observed between the C3435T polymorphism of the MDR1 gene and GC risk or prognosis in the population studied.

The Association between ABCB1 C1236T/C3435T SNPs and H. pylori Infection among Jordanians

“…Several studies have investigated the relationship between ABCB1 polymorphisms and H. pylori infection [13][14][15]. For instance, two Australian studies showed a strong association between them [16,17].…”

Association between ABCB1 Immunohistochemical Expression and Overall Survival in Gastric Cancer Patients

“…Furthermore, these polymorphisms may have a direct effect on ABCB1 gene expression and function of the ABCB1 protein (Fromm et al, 2002;Ambudkar et al, 2006Salama et al, 2006;Dean, 2009). Nevertheless, in previous studies conducted by our group (Oliveira et al, 2012;Wu et al, 2014), described a lack of association of the MDR1 C3435T polymorphism with susceptibility to GC. Differently, in another field study by Ren et al (2012), suggested that the MDR1 gene polymorphism was strongly associated with increased susceptibility to hepatocellular carcinoma.…”