Association between the 308 G&gt;A variant of the TNF-α gene and risk of colorectal cancer

Bani, Nastaran; Moetamani‐Ahmadi, Mehrdad; Alidoust, Maryam; Shahidsales, Soodabeh; Khazaei, Majid; Esmaily, Habibollah; Joudi‐Mashhad, Mona; Ferns, Gordon A.; Gharib, Masoumeh; Avan, Amir

doi:10.1016/j.mgene.2021.100878

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Standardizing the detection and quantification of biomarkers on HE slides, as a costeffective and widely available staining method, can greatly benefit diagnosis procedures, particularly in developing countries. We utilized the confirmed CRC data gathered by a previously conducted study [24]. One hundred and one cases with histological features of colorectal adenocarcinoma and available HE-stained sections were collected from the pathology department archives.…”

Section: Patientsmentioning

confidence: 99%

Assessment of the Tumor–Stroma Ratio and Tumor-Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation

et al. 2023

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Background: To implement the new marker in clinical practice, reliability assessment, validation, and standardization of utilization must be applied. This study evaluated the reliability of tumor-infiltrating lymphocytes (TILs) and tumor-stroma ratio (TSR) assessment through conventional microscopy by comparing observers’ estimations. Methods: Intratumoral and tumor-front stromal TILs, and TSR, were assessed by three pathologists using 86 CRC HE slides. TSR and TILs were categorized using one and four different proposed cutoff systems, respectively, and agreement was assessed using the intraclass coefficient (ICC) and Cohen’s kappa statistics. Pairwise evaluation of agreement was performed using the Fleiss kappa statistic and the concordance rate and it was visualized by Bland–Altman plots. To investigate the association between biomarkers and patient data, Pearson’s correlation analysis was applied. Results: For the evaluation of intratumoral stromal TILs, ICC of 0.505 (95% CI: 0.35–0.64) was obtained, kappa values were in the range of 0.21 to 0.38, and concordance rates in the range of 0.61 to 0.72. For the evaluation of tumor-front TILs, ICC was 0.52 (95% CI: 0.32–0.67), the overall kappa value ranged from 0.24 to 0.30, and the concordance rate ranged from 0.66 to 0.72. For estimating the TSR, the ICC was 0.48 (95% CI: 0.35–0.60), the kappa value was 0.49 and the concordance rate was 0.76. We observed a significant correlation between tumor grade and the median of TSR (0.29 (95% CI: 0.032–0.51), p-value = 0.03). Conclusions: The agreement between pathologists in estimating these markers corresponds to poor-to-moderate agreement; implementing immune scores in daily practice requires more concentration in inter-observer agreements.

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Section: Patientsmentioning

confidence: 99%

Assessment of the Tumor–Stroma Ratio and Tumor-Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation

et al. 2023

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Understanding the potential immunogenetic role of TNFα-308 polymorphism in the pathogenesis of recurrent miscarriage

Ali¹,

Majid²,

Ali³

et al. 2023

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Molecular Analysis of rs2910164 Polymorphism and its Association with TNF-α Gene Expression in Colorectal Cancer

Hamta

Tolooi

2021

Jentashapir J Cell Mol Biol

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Background: Colorectal cancer (CRC) is one of the most common malignant tumors in the world, which has been diagnosed as the third most common cancer and the third leading cause of death. This cancer before the age of 50 is uncommon, and its prognosis is controversial, with many studies reporting a worse prognosis than in older patients and others showing no difference. In addition to factors, including diet, environmental factors, somatic and hereditary mutations, genetic factors, including altered expression of some microRNAs and mutations in tumor necrosis factor-α (TNF-α) gene, may be involved in this cancer. Objectives: This study aimed to investigate the rs2910164 polymorphism from the miRNA146a gene and its association with the expression of TNF-α gene in CRC before and after the age of 50 for early diagnosis and treatment. Methods: In this study, 65 samples of cancerous lesions (37 cases over 50 years were considered the case group and 28 cases under 50 years were considered the control group) was collected from in an Iranian population. DNA was extracted from the samples, and rs2910164 polymorphism of the miRNA146a gene was investigated by PCR. Moreover, RNA was extracted from the samples, and the expression of the miRNA146a and TNF-α genes were evaluated. Finally, for data analysis, Epi Info software version 7.1.3.10 and MedCalc Version 19.2.0 were used. Results: The frequency of GG, GC, and CC genotypes from rs2910164 polymorphism of miRNA146a gene in the control group was 0.29, 0.46, and 0.25, respectively, but in the case group it was 0.54, 0.38, and 0.08, respectively. The results also showed that the expression of miRNA146a (P = 0.00006) and TNF-α (P = 0.009) genes was higher in the case group than the control group. Conclusions: Based on the results of this study, a significant correlation was found between GG genotype and rs2910164 polymorphism of miRNA146a gene and TNF-α gene expression in the CRC samples. Therefore, investigation of these factors may be helpful in patients over 50 years with CRC.

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Association between the 308 G>A variant of the TNF-α gene and risk of colorectal cancer

Cited by 3 publications

References 25 publications

Assessment of the Tumor–Stroma Ratio and Tumor-Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation

Assessment of the Tumor–Stroma Ratio and Tumor-Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation

Understanding the potential immunogenetic role of TNFα-308 polymorphism in the pathogenesis of recurrent miscarriage

Molecular Analysis of rs2910164 Polymorphism and its Association with TNF-α Gene Expression in Colorectal Cancer

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