Association between serum level of Vitamin D with autoantibodies expression, disease activity (SLEDAI) and bone mineral density (BMD) in patients with Systemic Lupus Erythematosus (SLE)

Kalim, Handono; Wahono, Singgih; Bp, Putra Suryana; Puspitasari, Lenny; Wijayanto, Fajar Hadi; Handono, Kusworini

doi:10.1186/ar3624

Cited by 3 publications

(1 citation statement)

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“…There is no data on the dominant type of autoantibodies produced by SLE patients in Indonesia because autoantibody profiling has never been done in this country. The most frequently reported autoantibodies in Indonesian patients with SLE are ANA and anti-dsDNA (Kalim et al, 2012).…”

Section: Development Of Sle Diagnostic Kitmentioning

confidence: 99%

Article Tools Print this article Indexing metadata How to cite item Email this article Email the author About The Authors Wisnu Barlianto Department of Pediatrics, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital, Malang, Indonesia Indonesia Hani Susianti Department of Clinical Pathology, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital, Malang, East Java, Indonesia Indonesia Singgih Wahono Department of Internal Medicine, Faculty of Medicine Brawijaya

Barlıanto

Susianti

Wahono

et al. 2017

RJLS

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Systemic Lupus Erythematosus (LES) isan autoimmune inflammatory disease characterized by the formation of antinuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) antibodies as diagnostic markers. Detection of such autoantibodies requires advanced equipment and trained personnel.This study was conducted to acquire candidate antigens that can be used for rapid test kit for practical and accurate detection of ANA and anti-dsDNA to speed up SLE diagnosis.Nuclear proteins and DNA derived from cell lines, hair follicles, and leukocytes of SLE patients and healthy individuals were isolated using QiaGEN kit and modifiedmanual procedure. Antigen-antibody bonds were tested by dot blot assay.The strongest binding between DNA antigens of a healthy individual and antibodies occurred at dilution factors of 1:5,120 for the antigen and 1:2,560 for the antibody. The strongest binding between nuclear protein antigens from the cell line and antibodies occurred at dilution factors of 1:512 for the antigen and 1:1,600 for the antibody.Nuclear antigens derived from cell line and DNA antigens of healthy individuals were antigen candidates for the development of ANA and anti-dsDNA rapid detection tests.

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Section: Development Of Sle Diagnostic Kitmentioning

confidence: 99%

Article Tools Print this article Indexing metadata How to cite item Email this article Email the author About The Authors Wisnu Barlianto Department of Pediatrics, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital, Malang, Indonesia Indonesia Hani Susianti Department of Clinical Pathology, Faculty of Medicine Brawijaya University/Dr. Saiful Anwar General Hospital, Malang, East Java, Indonesia Indonesia Singgih Wahono Department of Internal Medicine, Faculty of Medicine Brawijaya

Barlıanto

Susianti

Wahono

et al. 2017

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Progress report on the rapid test kit development for early detection of systemic lupus erythematosus in Indonesia

Handono

Benita

Habibah

et al. 2019

J. Phys.: Conf. Ser.

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Lower Levels of Vitamin D Associated with Disease Activity in Colombian Patients with Systemic Lupus Erythematosus

Ospina-Caicedo

Cardona-Rincón

Bello-Gualtero

et al. 2019

CRR

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Background: Systemic Lupus Erythematosus (SLE) involves genetic, environmental, and hormonal alterations, including Vitamin D deficiency. Objective: To evaluate the association between vitamin D levels with anti-dsDNA, complement proteins, immunoglobulins levels and disease activity scores. Methods: : A cross-sectional study was performed. The levels of 25-OH vitamin D were measured in patients older than 18 years with SLE according to ACR/97 [American College of Rheumatology 1997] from 2013 to 2015. The association was assessed by Mann-Whitney U and Kruskal Wallis tests for continuous variables, and by the Chi or Fisher exact test for the nominal variables. Results: Sixty-nine patients were included; 82% were women; the mean age was 38.5 years; 36.2% had low levels of vitamin D with higher consumption [p=0.006] of C4 and C3 complement proteins, plus higher levels of anti-dsDNA. Lower values of vitamin D were observed in patients with moderate to severe activity [p=0.0001] by SLEDAI [Systemic Lupus Erythematosus Activity Index] and general domain [p=0.039] and renal domain [p=0.009] by BILAG [British Isles Lupus Assessment Group] 2004. The mean vitamin D levels were higher in the group not receiving steroids when compared to those groups with dosages of 0.5-1mg/kg/d [p=0.048]. Conclusion: Lower levels of vitamin D are associated with greater complement protein consumption and higher disease activity rates. Therefore, it is important to evaluate vitamin D supplementation in patients with SLE as part of the treatment, especially when it includes the use of steroids.</P>

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Association between serum level of Vitamin D with autoantibodies expression, disease activity (SLEDAI) and bone mineral density (BMD) in patients with Systemic Lupus Erythematosus (SLE)

Cited by 3 publications

References 4 publications

Progress report on the rapid test kit development for early detection of systemic lupus erythematosus in Indonesia

Lower Levels of Vitamin D Associated with Disease Activity in Colombian Patients with Systemic Lupus Erythematosus

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