Association between selenium and lycopene supplementation and incidence of prostate cancer: Results from the post-hoc analysis of the procomb trial

Morgia, Giuseppe; Voce, S.; Palmieri, Fabiano; Gentile, Marcello; Iapicca, Gennaro; Giannantoni, Antonella; Blefari, F.; Carini, Marco; Vespasiani, Giuseppe; Santelli, G; Arnone, S.; Pareo, Rosaria M.; Russo, Giorgio Ivan

doi:10.1016/j.phymed.2017.06.008

Cited by 27 publications

(30 citation statements)

References 30 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The detailed procedure for Western blotting is described elsewhere [10]. Cells (1 × 10 6 ) were cultured in triplicate in six-well plates containing various concentration of diosgenin for 48 h. Total cell extracts were prepared in ice-cold lysis buffer (0.5% NP-40, 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, and 10% glycerol, pH 7.5) containing protease inhibitors (1 µg/mL aprotinin, 0.5 µg/mL leupeptin, and 100 µg/mL of 4-(2-aminoethyl)-benzenesulfonyl fluoride).…”

Section: Western Blot Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Induction of G2/M Phase Arrest by Diosgenin via Activation of Chk1 Kinase and Cdc25C Regulatory Pathways to Promote Apoptosis in Human Breast Cancer Cells

Liao

Lin

Shieh

et al. 2019

IJMS

View full text Add to dashboard Cite

The anti-tumor activity of diosgenin, a new steroidal constituent present in fenugreek, on two human breast cancer cell lines, MCF-7 and Hs578T, was studied. Diosgenin treatment resulted in cell growth inhibition, cell cycle arrest, and apoptosis in concentration- and time-dependent manners in both cell lines. Western blot analyses of whole cell lysates for cell cycle proteins showed that diosgenin altered phosphorylated cyclin checkpoint1 (p-Chk1Ser345) and cyclin B expression, which resulted in G2/M phase blockade. Mechanistically, Cdc25C-Cdc2 signaling was involved in inactivating Chk1Ser345 by p53-dependence in MCF-7 cells and p21-dependence in Hs578T cells that are p53-deficient. Moreover, diosgenin induced a significant loss of the mitochondrial membrane potential in breast cancer cells, and prominently affected cell death through down-regulation of the anti-apoptotic protein, Bcl-2. This released cytochrome c and activated the caspase signaling cascade. Taken together, these findings reveal that the anti-proliferative activity of diosgenin involves the induction of G2/M phase arrest via modulating the Cdc25C-Cdc2-cyclin B pathway and mitochondria-mediated apoptosis in human breast cancer cell lines. This suggests the potential usefulness of diosgenin in treating breast cancer.

show abstract

Section: Western Blot Analysesmentioning

confidence: 99%

“…A number of anticancer drugs, including camptothecin [2,3], docetaxel [4,5], licochalcone [6,7], polyphyllin I [8], and dioscin [9], are of plant origin. Recently, chemoprevention has been examined both in vitro and in vivo, and chemopreventive agents derived via phytomedicine have been validated in human intervention trials [10].…”

Section: Introductionmentioning

confidence: 99%

Induction of G2/M Phase Arrest by Diosgenin via Activation of Chk1 Kinase and Cdc25C Regulatory Pathways to Promote Apoptosis in Human Breast Cancer Cells

Liao

Lin

Shieh

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…11 Entretanto, ao comparar o uso da suplementação de compostos fitoquímicos em humanos com seu efeito protetor na diminuição do risco do câncer de próstata, duas pesquisas encontraram, em seus esboços investigativos, dados inconsistentes, não sendo capazes de sustentar a hipótese de que tais nutrientes estariam associados a um menor risco de neoplasia da próstata. 12,13 Os efeitos do licopeno no processo de oncogênese e na saúde da próstata são benéficos ao homem. Todavia, os ensaios clínicos de eficácia exigem uma adesão do referido composto bioativo em longo prazo e em elevadas concentrações, sendo necessários, concomitantemente, estudos que avaliem como potencializar sua absorção e maior biodisponibilidade e também as doses corretas de ingestão do mesmo para alcançar seu efeito protetor (►Quadro 3).…”

Section: Resultados E Discussõesunclassified

“…Todavia, os ensaios clínicos de eficácia exigem uma adesão do referido composto bioativo em longo prazo e em elevadas concentrações, sendo necessários, concomitantemente, estudos que avaliem como potencializar sua absorção e maior biodisponibilidade e também as doses corretas de ingestão do mesmo para alcançar seu efeito protetor (►Quadro 3). 12 Qiu et al, 14 ao estudarem os efeitos do licopeno na expressão das proteínas em células epiteliais prostáticas humanas, verificaram que o mesmo foi capaz de inibir a proliferação das células primárias do epitélio prostático por regulação da via proteína quinase B/proteína alvo da rapamicina em mamíferos (AKT/mTOR). Foi verificado, ainda, que o licopeno é capaz de alterar várias vias de sinalização, incluindo inibição da sinalização de andrógenos, a sinalização de fatores de necrose tumoral alfa (TNFα, na sigla em inglês), e a desativação da via proteíno-quinases ativadas por mitógenos (MAPK, na sigla em inglês).…”

Section: Resultados E Discussõesunclassified

Licopeno e prevenção do câncer de próstata: Uma revisão integrativa

Bôto¹,

Sala

Ferreira

et al. 2019

International Journal of Nutrology

View full text Add to dashboard Cite

ResumoAtualmente, o câncer de próstata configura-se como um importante problema de saúde pública, sendo um dos principais tipos de carcinoma diagnosticados em pacientes do sexo masculino, e tornando-se a causa primordial de morbimortalidade. Estudos têm evidenciado que hábitos e estilo de vida, juntamente com a instalação de um quadro de estresse oxidativo, apresentam-se como importantes fatores implicados no processo de carcinogênese. Em contrapartida, o hábito alimentar tem sido considerado como um aliado significativo na prevenção do câncer, por meio de grupos alimentares com propriedades antioxidantes e anti-inflamatórios capazes de atuar como compostos anticarcinogênicos, incluindo o licopeno, que é considerado um nutriente quimiopreventivo antioxidante. A presente revisão resume alguns resultados relevantes sobre o papel do licopeno na prevenção do câncer de próstata durante os últimos 7 anos. Foram selecionados artigos provenientes das seguintes bases de dados: Scielo, Lilacs, BVS, PubMed, e MEDLINE. De acordo com os critérios de inclusão e de exclusão, foram selecionados 20 artigos internacionais. Em revisões blibliográficas, ensaios clínicos (in vivo e in vitro) evidenciaram a influência da suplementação e do consumo de licopeno a partir de diferentes fontes de produtos à base de tomate e de outros alimentos-fontes na diminuição da viabilidade celular das células prostáticas após o tratamento, além de um aumento da apoptose e da regulação dos níveis transcricionais, apontando o licopeno como um importante fitoquímico. Entretanto, futuras triagens clínicas com o uso de licopeno devem ser realizadas para que seja possível estabelecer recomendações precisas sobre o consumo e a quantidade adequados do referido composto para que este seja utilizado para a prevenção do câncer de próstata.

show abstract

“…In a Cochrane review including randomized controlled trials and longitudinal observational studies, there was no association between selenium supplementation and the risk of prostate cancer [113], nor in a Mendelian randomization analysis by Yarmolinsky et al where the authors suggested that selenium supplementation could have unfavorable effects on risks of advanced disease Dietary Antioxidants in the Chemoprevention of Prostate Cancer DOI: http://dx.doi.org/10.5772/intechopen.85770 [114]. There is further supporting evidence in the follow-up of the Procomb trial where there was no association between selenium supplementation and prostate cancer risk [115]. Conversely, there are studies that suggest caution with selenium supplement usage among males with prostate cancer.…”

Section: Selenium and Prostate Cancermentioning

confidence: 99%

Dietary Antioxidants in the Chemoprevention of Prostate Cancer

Tucker¹,

Anderson²,

Miller³

et al. 2019

Antioxidants

View full text Add to dashboard Cite

Prostate cancer is the second most common cancer and the fifth leading cause of cancer death. The incidence of prostate cancer is rising due to increased screening and awareness, and there is epidemiological evidence suggesting an interaction among biological and environmental risk factors in the development and progression of prostate cancer. Vegetables and fruits provide a wide range of antioxidants and phytochemicals that have been demonstrated to have a negative, positive, or no association with prostate cancer risk. Therefore, it is evident that the effect of dietary antioxidants on risk of prostate cancer remains undecided and inconclusive. The main focus of this review was to examine recent and past literature of the chemoprotective properties of five major groups of phytochemicals against prostate cancer development including both in vivo and in vitro findings. 2 oxidative stress from reactive oxygen species and loss of antioxidant enzymes may contribute to genomic instability prior to prostate cancer [9]. This paper will review information in the literature on the relationship between nutrients with antioxidant properties from the diet, and the risk of prostate cancer. Method of article selectionA literature search was conducted for all English language literature published before December 2018. The search was conducted using the electronic databases, including PubMed, Embase, Web of Science, and Cochrane Library. The search strategy included keywords such as prostate cancer, epidemiology, incidence, mortality, risk factor, selenium, vitamin E, vitamin C, carotenoids, and polyphenols.The authors include many interventional and observational studies that have reported findings of dietary antioxidants, prostate cancer incidence, and progression. The majority of these studies focused on vitamins E and C, carotenoids, specifically beta-and alpha-carotene and lycopene, phenols including tea and coffee, and the flavonoids, as well as selenium.

show abstract

Association between selenium and lycopene supplementation and incidence of prostate cancer: Results from the post-hoc analysis of the procomb trial

Cited by 27 publications

References 30 publications

Induction of G2/M Phase Arrest by Diosgenin via Activation of Chk1 Kinase and Cdc25C Regulatory Pathways to Promote Apoptosis in Human Breast Cancer Cells

Induction of G2/M Phase Arrest by Diosgenin via Activation of Chk1 Kinase and Cdc25C Regulatory Pathways to Promote Apoptosis in Human Breast Cancer Cells

Licopeno e prevenção do câncer de próstata: Uma revisão integrativa

Dietary Antioxidants in the Chemoprevention of Prostate Cancer

Contact Info

Product

Resources

About