Background: Deterioration of physical function-a common feature of ageing-can lead to a lack of independence, poor quality of life, and entry into aged care. Given the aging population in Australia and worldwide, understanding how to maintain and improve function is a key public health concern. Resistance activities and other tailored exercise programs are effective at maintaining and improving function; however, older adults rarely engage in these programs. Understanding how activities across the intensity spectrum, in particular, time spent sedentary (sitting, reclining or lying down with low energy expenditure), impact on physical function can help to inform complementary approaches to the maintenance of physical function throughout life. Older adults spend, on average, nine hours per day sedentary, with those in aged care settings (such as nursing homes) averaging more than 12 hours of sedentary time daily. The detrimental health impacts of too much sitting are increasingly being recognised across a host of physical and mental health outcomes, including with physical function. However, to date, little research has been conducted that investigates the associations of sedentary time with physical function using posture monitors, in understudied populations such as aged care residents, and/or using longitudinal study designs. Aim: The primary aim of this Thesis is to investigate the association of sedentary time with physical function across settings (community-dwelling vs aged care), measurement methods (self-report and objective), and study designs (cross-sectional and longitudinal). This was achieved through five research objectives, which were to: • Systematically review the evidence on the associations of sedentary time with physical function in older adults (Chapter 2). • Investigate the cross-sectional associations of sedentary time with physical function in a community-dwelling sample using a posture-based monitor (Chapters 3 and 4). • Investigate the cross-sectional and longitudinal associations of sedentary time with physical function in the aged care setting (Chapter 5).