Association between Renal Podocalyxin Expression and Renal Dysfunction in Patients with Diabetic Nephropathy: A Single-Center, Retrospective Case-Control Study

Wang, Rongzhen; Yao, Cheng‐Bao; Liu, Feng

doi:10.1155/2020/7350781

Cited by 13 publications

(17 citation statements)

References 46 publications

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“…eGFR typically reflect impaired renal function only in the advanced stages of DN. In recent study, similar finding on correlation between eGFR and u-PDX was demonstrated [32].…”

Section: Discussionsupporting

confidence: 73%

Role of urinary podocalyxin in early diagnosis of diabetic nephropathy

Kostovska¹,

Tosheska-Trajkovska²,

Cekovska³

et al. 2020

Romanian Journal of Internal Medicine

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Introduction: Podocyte injury has been reported as an early feature of DN therefore, the assessment of podocyte injury can be accomplished by estimation of podocalyxin in urine. This study aimed to estimate the urinary podocalyxin levels and to determine the sensitivity and specificity of this biomarker for early detection of DN.Materials and methods: A total of 90 patients with type 2 diabetes mellitus (T2DM) were included in this cross-sectional study. Sixty of them were without diagnosed DN, and 30 with diagnosed DN. A control group consisted of 30 healthy subjects. All patients with T2DM were divided into three subgroups according to urinary microalbumin/creatinine ratio (UM/CR): normoalbuminuric, microalbuminuric and macroalbuminuric patients. Urine samples, were used for measurement of podocalyxin by ELISA, creatinine and microalbumin. Fasting venous blood samples was collected for biochemical analyses.Results: The levels of urinary podocalyxin (u-PDX) were higher in patients with T2DM compared to control subjects and a statistically significant difference among studied subgroups regarding u-PDX was found (p < 0.05). Levels of u-PDX are increasing gradually with the degree of DN (p < 0.029). u-PDX levels were positively correlated with UM/CR (r =0.227, p=0.002). A cut-off level of 43.8 ng/ml u-PDX showed 73.3% sensitivity and 93.3% specificity to detect DN in early stage. A cut-off level of 30 mg/g UM/CR showed 41.5% sensitivity and 90% specifity in predicting DN. u-PDX was elevated in 48,2% of normoalbuminuric patients.Conclusion: Urinary podocalyxin be useful and more sensitive and specific marker in early detection of DN than microalbuminuria.

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“…eGFR typically reflect impaired renal function only in the advanced stages of DN. In recent study, similar finding on correlation between eGFR and u-PDX was demonstrated [32].…”

Section: Discussionsupporting

confidence: 73%

Role of urinary podocalyxin in early diagnosis of diabetic nephropathy

Kostovska¹,

Tosheska-Trajkovska²,

Cekovska³

et al. 2020

Romanian Journal of Internal Medicine

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“…[18]. Данный показатель отражает повреждения клубочкового аппарата почек, при этом происходит усиленное выделение подоцитов с мочой [19]. Исследования ряда авторов показали, что подоцитурия развивается у 74% больных с нормоальбуминурией и 54% с микроальбуминурией, с одинаковой частотой при I и II типах СД [20].…”

Section: Discussionunclassified

“…проходит две стадии альбуминурии [21]. Таким образом, наши данные подтверждают ранее проведенные исследования о росте этого показателя у больных с ДН [19,20].…”

Section: Discussionunclassified

Markers of kidney injury, lipid metabolism, and carbonyl stress in patients with type 1 diabetes and different levels of albuminuria

et al. 2022

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The aim of this work was to study the levels of podocalyxin and β-2-microglobulin and parameters of lipid metabolism and carbonyl stress in type 1 diabetes mellitus (T1DM) patients with different levels of albuminuria.Materials and methods. 56 men of reproductive age with T1DM were divided into two groups: 24 patients with stage A1 albuminuria (group A1) and 32 patients with stage A2 albuminuria (group A2). The control group consisted of 28 healthy men. The levels of renal function markers, lipid metabolism parameters, and methylglyoxal were assessed using enzyme immunoassay and spectrophotometric and fluorometric methods.Results. Higher values for total cholesterol, triacylglycerol, and very-low-density lipoprotein medians in both groups A1 and A2 were found. In these groups, increased podocalyxin and methylglyoxal medians were revealed. Correlation analysis in the group A1 showed the presence of a relationship between the glomerular filtration rate (GFR) and creatinine. In the group A2, correlations between the generally accepted parameters of kidney injury (the albumin / creatinine ratio and GFR) and the duration of the disease and between GFR and the creatinine and methylglyoxal levels in the blood were identified. The podocalyxin level in this group correlated with the β2- microglobulin and methylglyoxal levels and lipid metabolism parameters. The level of β2-microglobulin correlated with the lipid metabolism parameters.Conclusion. Regardless of the level of albuminuria, men with T1DM had significantly increased levels of podocalyxin, lipid metabolism parameters, and methylglyoxal, as well as strong relationships between these parameters. The data of this study can be used for development of potential strategies for prevention and early treatment of diabetic nephropathy.

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“…Another study reports a heterozygous nonsense mutation in podocalyxin gene (c.C976T; p. Arg326X) that is characterized by proteinuria and renal insufficiency (189). Interestingly, a strong association was found between poor renal outcome and reduction of podocalyxin expression with an increase in its urinary excretion (342). Thus, assessment of podocalyxin levels in renal tissues and urine was proposed as a reliable biomarker to predict the progression of DN.…”

Section: Glomerulusmentioning

confidence: 99%

Renal cell markers: lighthouses for managing renal diseases

Agarwal

Sudhini

Polat

et al. 2021

American Journal of Physiology-Renal Physiology

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Kidneys, one of the vital organs in our body, are responsible for maintaining whole-body homeostasis. The complexity of renal function (e.g., filtration, reabsorption, fluid and electrolyte regulation, urine production) demands diversity not only at the level of cell types but also in their overall distribution and structural framework within the kidney. To gain an in-depth molecular-level understanding of the renal system, it is imperative to discern the components of kidney and the types of cells residing in each of the sub-regions. Recent developments in labeling, tracing, and imaging techniques enabled us to mark, monitor and identify these cells in vivo with high efficiency in a minimally invasive manner. In this review, we have summarized different cell types, specific markers that are uniquely associated with those cell types, and their distribution in kidney, which altogether make kidneys so special and different. Cellular sorting based on the presence of certain proteins on the cell surface allowed for assignment of multiple markers for each cell type. However, different studies using different techniques have found contradictions in the cell-type specific markers. Thus, the term "cell marker" might be imprecise and sub-optimal, leading to uncertainty when interpreting the data. Therefore, we strongly believe that there is an unmet need to define the best cell markers for a cell type. Although, the compendium of renal-selective marker proteins presented in this review is a resource that may be useful to the researchers, we acknowledge that the list may not be necessarily exhaustive.

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Association between Renal Podocalyxin Expression and Renal Dysfunction in Patients with Diabetic Nephropathy: A Single-Center, Retrospective Case-Control Study

Cited by 13 publications

References 46 publications

Role of urinary podocalyxin in early diagnosis of diabetic nephropathy

Role of urinary podocalyxin in early diagnosis of diabetic nephropathy

Markers of kidney injury, lipid metabolism, and carbonyl stress in patients with type 1 diabetes and different levels of albuminuria

Renal cell markers: lighthouses for managing renal diseases

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