Association between reactive oxygen species and disease activity in chronic hepatitis C

Maria, Nicola De; Colantonl, Alessandra; Fagiuoli, Stefano; Liu, Guangjun; Rogers, Brad K.; Farinati, Fabio; Thiel, David H. Van; Floyd, Robert A.

doi:10.1016/0891-5849(96)00044-5

Cited by 212 publications

(162 citation statements)

References 13 publications

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“…2,3,25 Previous results from our own group have shown that antioxidants such as N-acetylcysteine, administered together with IFN, induced a significant decrease in serum transaminases (although not clearance of viremia) in patients previously resistant to IFN alone. 25 In this article we show that the transcriptional expression of Mn-SOD is increased in PBMC from patients with chronic hepatitis C. In this disease, both PBMC and the liver have been shown to be infected by HCV.…”

Section: Discussionmentioning

confidence: 95%

“…It has been suggested that HCV may cause oxidative stress in infected cells. Several lines of evidence support this contention, including the existence of an activated glutathione turnover, the presence of increased levels of lipid peroxidation products and augmented iron stores in the liver, [2][3][4] and the finding of diminished reduced glutathione values in peripheral blood mononuclear cells (PBMC) and erythrocytes. 5,6 Moreover, it has been shown that patients with chronic hepatitis C exhibit an increased production of tumor necrosis factor-␣ (TNF␣), 7 a cytokine that can produce oxidative stress by stimulating the generation of reactive oxygen species (ROS) such as superoxide ion (O 2 ⅐Ϫ ) and hydrogen peroxide (H 2 O 2 ).…”

Section: Introductionmentioning

confidence: 99%

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Superoxide Dismutase in Patients With Chronic Hepatitis C Virus Infection

Larrea

Beloqui

Muñoz-Navas

et al. 1998

Free Radical Biology and Medicine

109

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Abstract-It has been reported that hepatitis C virus (HCV) may cause oxidative stress in infected cells. Patients with chronic hepatitis C exhibit an increased production of tumor necrosis factor-␣ (TNF␣), a cytokine that can produce oxidative stress by stimulating the generation of reactive oxygen species (ROS). Cell defense against ROS includes overexpression of Mn-superoxide dismutase (SOD), an inducible mitochondrial enzyme. To investigate cell defense against oxidative stress in HCV infection, we analyzed Mn-SOD mRNA in liver and in peripheral blood mononuclear cells (PBMC) from patients with chronic hepatitis C. Mn-SOD expression in PBMC was significantly increased in patients with HCV infection. Patients with sustained virological and biochemical response after therapy showed significantly lower Mn-SOD than patients with positive viremia. By contrast, Mn-SOD expression was not enhanced in the liver of patients with chronic hepatitis C. The values of Mn-SOD mRNA did not correlate with TNF␣ mRNA expression, viral load, or liver disease activity. Our results indicate that in HCV infection an induction of Mn-SOD was present in PBMC but absent in the liver, suggesting that this organ could be less protected against oxidative damage. Oxidative stress could participate in the pathogenesis of HCV infection.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Superoxide Dismutase in Patients With Chronic Hepatitis C Virus Infection

Larrea

Beloqui

Muñoz-Navas

et al. 1998

Free Radical Biology and Medicine

109

View full text Add to dashboard Cite

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“…Recent studies have documented an association between HCV infection and the presence of both liver and serum markers of oxidative stress. [9][10][11][12][13][14] Furthermore, the expression of HCV core protein in transgenic mice or in hepatoma cells lines alters the liver antioxidant status and promotes lipid peroxidation. 15,16 Ethanol is also known to stimulate the production of reactive oxygen species and hydroxyethyl free radicals and to lower hepatic antioxidant defense.…”

mentioning

confidence: 99%

Moderate alcohol consumption increases oxidative stress in patients with chronic hepatitis C

et al. 2003

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The mechanisms by which alcohol consumption worsens the evolution of chronic hepatitis C (CHC) are poorly understood. We have investigated the possible interaction between hepatitis C virus (HCV) and ethanol in promoting oxidative stress. Circulating IgG against human serum albumin (HSA) adducted with malondialdehyde (MDA-HSA), 4-hydroxynonenal (HNE-HSA), or arachidonic acid hydroperoxide (AAHP-HSA) and against oxidized cardiolipin (Ox-CL) were evaluated as markers of oxidative stress in 145 CHC patients with different alcohol consumption, 20 HCV-free heavy drinkers (HD) without liver disease, and 50 healthy controls. Anti-MDA IgG was increased in CHC patients irrespective of alcohol intake as well as in the HD group. CHC patients with moderate alcohol intake (<50 g ethanol/d), but not HD, also had significantly higher values of anti-AAHP-HSA, anti-HNE-HSA, and anti-Ox-CL IgG (P < .05) than controls. A further elevation (P < .001) of these antibodies was evident in CHC patients with heavy alcohol intake (>50 g ethanol/d). Anti-AAHP and anti-Ox-CL IgG above the 95th percentile in the controls were observed in 24% to 26% of moderate and 58% to 63% of heavy drinkers but only in 6% to 9% of the abstainers. The risk of developing oxidative stress during CHC was increased 3-fold by moderate and 13-to 24-fold by heavy alcohol consumption. Heavy drinking CHC patients had significantly more piecemeal necrosis and fibrosis than abstainers. Diffuse piecemeal necrosis was 4-fold more frequent among alcohol-consuming patients with lipid peroxidation-related antibodies than among those without these antibodies. In conclusion, even moderate alcohol consumption promotes oxidative stress in CHC patients, suggesting a role for oxidative injury in the worsening of CHC evolution by alcohol. (HEPATOLOGY 2003; 38:42-49.)

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“…The oxidative status of subjects was determined by also measuring MDA, a well known and accepted OS marker, which showed a significant increase in HCV (+) HD patients when compared with HCV (-) HD patients and controls. These findings indicate that HCV infection which by itself is known to cause oxidative stress 15,16 is further augmenting this state of oxidative stress in ESRD patients on maintenance HD. It is well established that ESRD by itself is also associated with OS.…”

Section: Discussionmentioning

confidence: 70%

“…1 It has been shown that HCV infection by itself is characterized by an increase in free radical formation manifested by increased hepatic and serum levels of products of lipid peroxidation. 2,3 Earlier studies have suggested and proposed Ischemia-modified albumin (IMA) to be a sensitive biomarker of cardiac ischemia. 4,5 However recently the role and diagnostic significance of IMA in disorders of non-cardiac origin are being explored.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ischemia Modified Albumin in Hepatitis C Positive Hemodialysis Patients : A Pilot Study

Kumar

Suchitra

et al. 2012

Int J of Biomed & Adv Res

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Background: Chronic hemodialysis (HD) patients are more prone for infection such as Hepatitis C virus (HCV) infection, which may be associated with increased oxidative stress (OS). Ischemiamodified albumin (IMA) levels rises not only during ischemia but also during chronic OS. Hence the study was aimed to evaluate the levels of IMA and its association with oxidative stress in HCV positive HD patients. Methods: Twenty two HCV positive [HCV (+)], 22 HCV negative [HCV (-)] patients with end stage renal disease (ESRD) on maintenance HD and 22 healthy subjects were enrolled in this crosssectional observational study. Plasma IMA was determined by spectrophotometric Co (II) -albumin binding assay. Plasma MDA was determined by spectrophotometric method. Results: Both MDA and IMA levels were significantly higher in HCV (+) and HCV (-) HD patients compared to controls (p < 0.001). A significant increase in MDA levels was observed in HCV (+) HD patients when compared with HCV (-) HD patients (p<0.001); however no change in IMA levels were noted between HCV (+) and HCV (-) HD patients. A negative correlation was observed between IMA and MDA levels in both HCV (+) (r= -0.278, p=0.178) and HCV (-) HD patients (r= -0.193, p=0.354) which were however not statistically significant. Conclusion: Plasma IMA and MDA levels were elevated in both groups of HD patients when compared to controls. However, hepatitis C infection does not appear to cause any additional increase of IMA levels in HD patients.

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Association between reactive oxygen species and disease activity in chronic hepatitis C

Cited by 212 publications

References 13 publications

Superoxide Dismutase in Patients With Chronic Hepatitis C Virus Infection

Superoxide Dismutase in Patients With Chronic Hepatitis C Virus Infection

Moderate alcohol consumption increases oxidative stress in patients with chronic hepatitis C

Evaluation of Ischemia Modified Albumin in Hepatitis C Positive Hemodialysis Patients : A Pilot Study

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