Association between Programmed Cell Death 6 Interacting Protein Insertion/Deletion Polymorphism and the Risk of Breast Cancer in a Sample of Iranian Population

Hashemi, Mohammad; Yousefi, Javad; Hashemi, Seyed Mehdi; Amininia, Shadi; Ebrahimi, Mahdi; Taheri, Mohsen; Ghavami, Saeid

doi:10.1155/2015/854621

Cited by 13 publications

(11 citation statements)

References 29 publications

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“…Some risk factors including early menarche, late menopause occurred, body mass index (BMI) and obesity after menopause, estrogen intake, alcohol consumption and tobacco use, exposure to ionizing radiation, and eating habits (3), as well as genetic (4)(5)(6), environmental factors (5,6), and ethnicity (7) can effect on the incidence of BC. In various studies, it was found that the serum levels of trace elements in patients with BC have changed and although their role is not fully understood, it may be associated with the risk of this disease (8).…”

Section: Introductionmentioning

confidence: 99%

Serum Levels of Selenium and Zinc in Patients with Breast Cancer: A Case-Control Study

et al. 2017

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Background: Zinc (Zn) and Selenium (Se) are trace minerals that have high anti-cancer and chemopreventive properties. Objectives: This study aimed at evaluating the serum levels of both elements in women with breast cancer (BC) compared with control group and the correlation of them with risk factors of BC. Methods: In a case-control study, 142 women with BC and 158 healthy controls, aged 19 to 88 years, were selected. Both groups did not use any type of supplement 3 months before participation in the study; there was no history of chronic kidney or liver disease or malabsorption disorders among the participants and body mass index was between 18.5 and 30 based on Quetelet index. The Se by graphite furnace and Zn by flame were atomized and the amount of each of 2 elements was measured and recorded by the Absorption device.

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Section: Introductionmentioning

confidence: 99%

Serum Levels of Selenium and Zinc in Patients with Breast Cancer: A Case-Control Study

et al. 2017

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“…The interaction between miRNA-124-3p and PDCD6 was then verified using a dual Luciferase reporter assay system. PDCD6 is a calcium-binding modulator protein that regulates cell proliferation and death (23,24). Aberrant expression of PDCD6 has been observed in various types of human cancer and may act as either an oncogene or a tumor suppressor.…”

Section: Discussionmentioning

confidence: 99%

“…2A). PDCD6 is a calcium-binding modulator protein, which regulates cell proliferation and apoptosis (23,24), indicating a promising role in CH development. Therefore, PDCD6 was used for subsequent experiments.…”

Section: Mir-124-3p Was Downregulated In Rats With Chmentioning

confidence: 99%

microRNA‑124‑3p inhibits the progression of congenital hypothyroidism via targeting programmed cell death protein 6

Song

Sun

et al. 2018

Exp Ther Med

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Abstract. The incidence of congenital hypothyroidism (CH) in newborn infants ranges from 1 in 3,000 to 1 in 4,000. Previous studies have indicated the neuroprotective role of microRNA (miR)-124-3p, however the expression and role of miR-124-3p in CH remain unclear. Therefore, the present study was performed to investigate the role and precise molecular mechanism of miR-124-3p in CH. Propylthiouracil (50 mg/day) was injected into the stomach of pregnant rats from gestational day 15 until parturition in order to establish a thyroid hypofunction model. Newborn rats were divided into the following four groups: The control group; the thyroid hypofunction group; the miR-124-3p mimic group; and the miR-124-3p negative control group. Reverse transcription-quantitative polymerase chain reaction indicated that miR-124-3p was significantly decreased in the hippocampus of the thyroid hypofunction group compared with the control group. Bioinformatics software was used to predict mRNA targets recognized by miR-124-3p and the programmed cell death protein 6 (PDCD6) 3' untranslated region (UTR) was demonstrated to exhibit the seed sequence of miR-124-3p. The interaction between miRNA-124-3p and PDCD6 was then verified using a dual-luciferase reporter assay system. PDCD6 expression was significantly increased in the hippocampus of rats with CH compared with the control group. Flow cytometry was performed to investigate the effects of miR-124-3p on neuronal cell apoptosis and the results indicated that the apoptosis rate in the thyroid hypofunction group was significantly increased compared with the control group; this increase was reversed by transfection with miR-124-3p mimics. Western blot analysis was used to detect the levels of cleaved poly [ADP-ribose] polymerase (PARP), full-length PARP, caspase-3, B cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) proteins. The results indicated that the expression of cleaved PARP, caspase-3 and Bax protein were significantly increased and the expression of full-length PARP and Bcl-2 protein was significantly decreased compared with the control group. These effects were reversed by miRNA-124-3p mimic transfection. Taken together, the results of the present study demonstrate that miRNA-124-3p serves a protective role in CH via targeting PDCD6.

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“…Ethical approvals for recruitment were taken from local Ethics Committee of Zahedan University of Medical Sciences, and informed consent was obtained from all patients and healthy individuals. The study design and enrolment procedure were described previously [29][30][31].…”

Section: Methodsmentioning

confidence: 99%

miR-608 rs4919510 C>G POLYMORPHISM DECREASED THE RISK OF BREAST CANCER IN AN IRANIAN SUBPOPULATION

et al. 2016

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Aim: MicroRNAs (miRNAs) are small noncoding RNAs that function as oncogene or tumor suppressors. The single nucleotide polymorphisms in miRNAs potentially can alter miRNA-binding sites on target genes as well as affecting miRNAs expression. The present study aimed to evaluate the impact of miR-608 rs4919510 C>G variant on breast cancer (BC) risk. Materials and Me thods: This case-control study conducted on 160 women with BC and 192 age-matched healthy women. Genotyping of miR608 rs4919510 was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: Our findings showed that GC genotype significantly decreased the risk of BC (odds ratio (OR) = 0.49, 95% confidence interval (CI) 0.28–0.88, p = 0.018) compared to CC genotype. Furthermore the G allele decreased the risk of BC (OR = 0.53, 95%CI 0.30–0.92, p = 0.024). No significant association was found between miR-609 genotypes and clinicopathological characteristics of BC patients (p > 0.05). Conclusion: Our findings indicate that miR-608 polymorphism might be associated with decreased risk of BC in an Iranian subpopulation. Further large-scale studies with different ethnicities are needed to verify our findings.

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Association between Programmed Cell Death 6 Interacting Protein Insertion/Deletion Polymorphism and the Risk of Breast Cancer in a Sample of Iranian Population

Cited by 13 publications

References 29 publications

Serum Levels of Selenium and Zinc in Patients with Breast Cancer: A Case-Control Study

Serum Levels of Selenium and Zinc in Patients with Breast Cancer: A Case-Control Study

microRNA‑124‑3p inhibits the progression of congenital hypothyroidism via targeting programmed cell death protein 6

miR-608 rs4919510 C>G POLYMORPHISM DECREASED THE RISK OF BREAST CANCER IN AN IRANIAN SUBPOPULATION

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