Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA

Riou, G; Favre, Michel; Jeannel, Dominique; Bourhis, Jean; Doussal, V. Le; Orth, Gérard

doi:10.1016/0140-6736(90)92693-c

Cited by 249 publications

(153 citation statements)

References 18 publications

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“…Franco (1994) reported that this figure was still being scrutinised by the authors and it was likely that additional HPV-positive samples would be declared, raising the detection rate to very close to 100%. Such a result would be at odds with previous observations that HPV-negative and HPV-positive patients with squamous cell cancer of the cervix differ with respect to age and prognosis (Higgins et al, 1991;Riou et al, 1990). Opinions regarding the prevalence of HPV in CIN are fairly consistent.…”

mentioning

confidence: 87%

Epidemiological evidence of cervical intraepithelial neoplasia without the presence of human papillomavirus

Burger¹,

Hollema²,

Pieters³

et al. 1996

Br J Cancer

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Summary The aim of this paper was to provide epidemiological evidence to support the notion that cervical intraepithelial neoplasia (CIN) without human papillomavirus (HPV) is a true entity. If a diagnosis of HPVnegative cervical neoplasia is erroneous, one would not expect there to be any differences in risk factors between HPV-positive and HPV-negative patients. Patients at a gynaecological outpatient clinic of a university hospital [a total of 265 consecutive women with dyskaryotic cervical smears who were subsequently diagnosed with CIN I (n = 37), CIN II (n = 48) or CIN III (n = 180)] completed a structured questionnaire regarding smoking habits and sexual history. Analysis of an endocervical swab for Chlamydia trachomatis, analysis of a cervical scrape for HPV, and morphological examination of cervical biopsy specimens were also performed. HPV was found in 205 (77.4%) out of the 265 women. Univariate analysis showed that current age (P= 0.02), current smoking behaviour (P = 0.002) and the number of sexual partners (P = 0.02) were significantly associated with the presence of HPV. Age at first sexual intercourse, a past history of venereal disease or genital warts, and current infection with Chiamydia trachomatis were not associated with the presence of HPV. Using multivariate logistic regression analysis, the number of sexual partners and current smoking behaviour showed an independent significant association with HPV. HPV-negative and HPV-positive CIN patients differ with respect to the risk factors for HPV. These findings suggest that HPV-negative CIN is a separate true entity.Keywords: cervix dysplasia; papillomavirus; risk factor Cervical intraepithelial neoplasia (CIN) is a morphologically defined lesion associated with the development of cervical carcinoma. In the conventional morphogenetic model CIN is separated into three grades according to the degree of cellular atypia and disturbance of the epithelial architecture (Richart, 1973). The infection with human papillomavirus (HPV) is strongly associated with cervical neoplasia. HPV shows considerable genetic heterogeneity (De Villiers, 1989), and a great diversity of HPV types is found in CIN Lungu et al., 1992; Bergeron et al., 1992). Known risk factors for cervical HPV infection are a comparatively young age and an increased lifetime number of sexual partners (Schiffman, 1994;Woodman, 1994). Smoking has also been identified as a risk factor 95%. Franco (1994) reported that this figure was still being scrutinised by the authors and it was likely that additional HPV-positive samples would be declared, raising the detection rate to very close to 100%. Such a result would be at odds with previous observations that HPV-negative and HPV-positive patients with squamous cell cancer of the cervix differ with respect to age and prognosis (Higgins et al., 1991;Riou et al., 1990). Opinions regarding the prevalence of HPV in CIN are fairly consistent. Schiffman (1994) mentioned that in their studies the prevalence of HPV in definite cases of CIN approached ...

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confidence: 87%

Epidemiological evidence of cervical intraepithelial neoplasia without the presence of human papillomavirus

Burger¹,

Hollema²,

Pieters³

et al. 1996

Br J Cancer

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“…Certain studies have demonstrated an association between HPV 18-related cervical cancer and a poor prognosis (6,7). However, in previous studies, HPV-positive cervical cancer patients demonstrated a more favorable prognosis than HPV-negative patients (8,9). Whereas certain authors were unable to find any significance between HPV types and their prognosis (10).…”

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confidence: 95%

“…However, another study indicated the adverse prognosis of PDGF-AA in pancreatic cancer (26), thus the prognostic value of PDGF-AA in CSCC requires further investigation. However, the prognostic value of HPV remains controversial (6)(7)(8)(9)(10). Studies have reported the prognostic value of HGF in patients with head and neck squamous cell carcinoma and verified that the high expression of HGF was closely associated with a poor prognosis (45).…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

Expression of growth-regulated oncogene-1, hepatocyte growth factor, platelet-derived growth factor-AA and soluble E-selectin and their association with high-risk human papillomavirus infection in squamous cell carcinoma of the uterine cervix

Zhang

Yang

et al. 2014

Molecular Medicine Reports

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Abstract. The aim of the present study was to evaluate the clinical significance and prognostic value of growth-regulated oncogene-1 (GRO-1), hepatocyte growth factor (HGF), platelet-derived growth factor-AA (PDGF-AA), soluble E-selectin (sE-selectin) and high-risk human papillomavirus (HPV; types 16, 18/45, 31 and 33/52/58/67) infection in cervical squamous cell carcinoma (CSCC). A total of 426 cases were enrolled in the present study, of which 292 cases were patients with CSCC, 43 were patients with cervical intraepithelial neoplasia (CIN) and 91 were healthy controls. Luminex xMAP technology was used to detect the serum levels of GRO-1, HGF, PDGF-AA and sE-selectin in all cases and two-channel fluorescence quantitative polymerase chain reaction was used to determine HPV DNA in cervical scrapings from CSCC and CIN patients. The results demonstrated that the serum levels of GRO-1, HGF and sE-selectin were significantly higher in patients with CSCC compared with patients with CIN and the healthy controls (P<0.0001). Compared with the CIN patients, the HPV positive rate in the CSCC patients significantly increased (P= 0.013). The four factors were correlated with certain clinicopathological variables of CSCC patients to a certain degree (P<0.05) and the levels of HGF were closely associated with HPV infection (P= 0.039). The receiver operating characteristic curves demonstrated that HGF obtained the highest diagnostic value compared with the other three factors. Multivariate Cox regression analysis demonstrated that the serum levels of HGF (P<0.0001), FIGO stage (P<0.0001) and pelvic lymph node metastasis (P=0.001) were independent prognostic factors in patients with CSCC, while high-risk HPV infection did not show any significance in this analysis. These results demonstrated that HGF may be a useful prognostic biomarker rather than high-risk HPV types in patients with CSCC.

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“…Furthermore, the majority of malignant lesions are infected with the "high risk" genotypes HPV-16 or HPV-18 (26,32,34).…”

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confidence: 99%

Detection of human papillomavirus type 16/18 DNA in cervicovaginal cells by fluorescence based in situ hybridization and automated image cytometry

et al. 1994

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Automatic fluorescence image cytometry (AFIC) is a fast, sensitive, and reliable approach for screening slide-based clinical specimens. In this study, we applied AFIC to identify cancer-associated human papillomavirus (HPV) genotypes 16 and 18 in individual cells of cervical smears using a sensitive fluorescence based in situ hybridization (FISH) assay. HPV sequences were labeled by FISH and the cells imaged using an epi-fluorescence microscope coupled to a low-light color CCD camera. Before application to clinical specimens, AFIC was assessed using fluorescent calibration beads and cervical cancer cell lines containing known numbers of integrated HPV genomes per nucleus. Assessment showed that our AFIC had a linear response, was quantitatively accurate, and had the sensitivity to detect one HPV genome per nucleus. After acquisition of images, computer algorithms identified every cell nucleus (via a fluorescent DNA counterstain) and quantified the FISH signal per nucleus. AFIC was employed to screen 27 patient specimens for HPV 16/18, of which 12 were positive. The HPV status of the specimens positively correlated with the pathological diagnosis, and since AFIC automatically and correctly located every cell, it was possible to directly compare morphology and HPV status in the same cell. In conclusion, the combination of FISH and AFIC is a sensitive and quantitative method to detect high risk HPV sequences in cervical Smears. Q 1994 Wiley-Liss, Inc.Key terms: Fluorescent in situ hybridization, cervical smears, human papillomavirus, automated fluorescence image cytometry Cervical dysplasia and carcinoma are common among women throughout the world. Approximately 500,000 women worldwide are diagnosed annually as having cervical cancer (24) and the mortality rate associated with this malignancy is second to breast cancer (20). In the United States, it is estimated that 13,000 women develop invasive cervical cancer annually (25).Substantial evidence has accumulated associating specific human papillomaviruses (HPVs) with human anogenital disorders, most notably cervical cancer (3,11,13,35). To date, over 60 different types of HPV have been described, of which about 20 have been associated with anogenital lesions (8). These 20 HPVs have been divided into "high risk" and "low risk" groups based on their association with benign vs. ma-'Supported by grants from NIH (CA51323 and the Office of Research on Womens Health), the Whitaker Foundation, and a contract with American Innovision, Inc.

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Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA

Cited by 249 publications

References 18 publications

Epidemiological evidence of cervical intraepithelial neoplasia without the presence of human papillomavirus

Epidemiological evidence of cervical intraepithelial neoplasia without the presence of human papillomavirus

Expression of growth-regulated oncogene-1, hepatocyte growth factor, platelet-derived growth factor-AA and soluble E-selectin and their association with high-risk human papillomavirus infection in squamous cell carcinoma of the uterine cervix

Detection of human papillomavirus type 16/18 DNA in cervicovaginal cells by fluorescence based in situ hybridization and automated image cytometry

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