Association Between Oxidative Stress and Melanoma Progression

Abstract

SummaryBackground: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusion: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level.

Oxidative stress and antioxidants in the pathophysiology of malignant melanoma

“…However, total serum SOD activity was only elevated significantly in Stage III melanoma, while SOD2 activity was only elevated in Stage IV melanoma. Catalase activity was elevated in Stage I, II, and III melanoma but not in Stage IV melanoma patients as compared to the healthy control group (Bisevac et al, 2018). A recent study further validated these observations (Santos Bernardes et al, 2016).…”

“…As oxidative stress is also a hallmark for aging, thus it is possible that aging masked some differences between the melanoma and healthy groups. In patients, the serum MDA levels are elevated in all stages of melanoma, and patients with Stage IV tumors showed the highest levels as compared to other stages (Bisevac et al, 2018). Serum superoxide levels were significantly elevated in melanoma patients compared with control healthy subjects, and the levels increase with stages of melanoma (Bisevac et al, 2018).…”

“…In patients, the serum MDA levels are elevated in all stages of melanoma, and patients with Stage IV tumors showed the highest levels as compared to other stages (Bisevac et al, 2018). Serum superoxide levels were significantly elevated in melanoma patients compared with control healthy subjects, and the levels increase with stages of melanoma (Bisevac et al, 2018). However, total serum SOD activity was only elevated significantly in Stage III melanoma, while SOD2 activity was only elevated in Stage IV melanoma.…”

Effect of Sulforaphane and 5-Aza-2’-Deoxycytidine on Melanoma Cell Growth

“…These impacts include inhibition of p27, cell cycle regulation, increased cytokines, decreased antioxidant glutathione s-transferase, increased 8-oxoG, activation of mitogen-activated protein kinase/extracellular signal-regulated kinases1/2 (MAPK/ERK1/2), increased cell proliferation and decreased tumor suppressor gene p16 [5]. These findings support the fact that melanoma patients have a higher level of oxidative stress and that this stress is associated with the progression of the disease [23].…”

Primary malignant melanoma of the biliary tract: A case report and literature review

“…Ultraviolet radiation (UVR) is the most significant modifiable risk factor. Both UVA-A and UVR-B have genotoxic effects either because of generation of oxygen radicals or abnormality in the nucleotide sequence, among others[21]. Melanomas are mainly classified according to their location and relationship to solar exposure[2].…”