Association between obesity and sperm quality

Ramaraju, G. A.; Teppala, Srinivas; Prathigudupu, K.; Kalagara, M.; Thota, S.; Krishna, Kota Murali; Cheemakurthi, R.

doi:10.1111/and.12888

Cited by 63 publications

(48 citation statements)

References 91 publications

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Section: Discussionmentioning

confidence: 99%

“…The rate of infertility has recently risen as has the alarming increase in obesity (Petraglia, Serour, & Chapron, 2013; Ramaraju et al, 2018). Many studies have shown that there is a correlation between male obesity and sperm count (Campbell et al, 2015; Ma et al, 2019; Ramaraju et al, 2018). Endocrine disorders, inherited, physical, or chemical factors are involved in the decline of reproductive capacity in obesity (Alves et al, 2016; Craig et al, 2017; Hayden et al, 2018; Sifakis et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The rate of infertility has recently risen as has the alarming increase in obesity (Petraglia, Serour, & Chapron, 2013; Ramaraju et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have shown that there is a correlation between male obesity and sperm count (Campbell et al, 2015;Ma et al, 2019;Ramaraju et al, 2018). Endocrine disorders, inherited, physical, or chemical factors are involved in the decline of reproductive capacity in obesity Craig et al, 2017;Hayden et al, 2018;Sifakis et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Over the past few decades, the adverse effects of obesity on male fertility have been considered a health problem. Many epidemiological studies have shown that body mass index is negatively correlated with male reproductive potential (Campbell, Lane, Owens, & Bakos, 2015; Ma et al, 2019; Ramaraju et al, 2018). The mechanisms by which obesity causes a decline in reproductive capacity are complex, involving endocrine disorders, inherited, physical, or chemical factors (Alves et al, 2016; Craig, Jenkins, Carrell, & Hotaling, 2017; Hayden, Flannigan, & Schlegel, 2018; Sifakis, Androutsopoulos, Tsatsakis, & Spandidos, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Adenosine accumulation causes metabolic disorders in testes and associates with lower testosterone level in obese mice

Yang

Zhao

Sun

et al. 2020

Molecular Reproduction Devel

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Overweight and obese men face numerous health problems, including type 2 diabetes, subfertility, and even infertility. However, few studies have focused on the effects of nutritional status and obesity‐related regulatory signals on fertility deficiency. Our previous observations have shown that the elevation of plasma 5'‐adenosine monophosphate (5'‐AMP) and the accumulation of adenosine in liver and muscle of obese diabetic db/db mice are related to insulin resistance. Here, we found that adenosine accumulation in testis is a common marker of both genetic obesity and high‐fat‐diet induced obese mice. An messenger RNA sequencing analysis indicated that 78 upregulated genes and 155 downregulated genes in the testis of 5'‐AMP‐treated mice overlapped with the same genes in the testis of ob/ob mice, and these genes belonged to the clusters of steroid metabolic process and regulation of hormone levels, respectively. Serum testosterone was reduced in ob/ob and 5'‐AMP‐treated mice. Metabolomic analysis based on 1H nuclear magnetic resonance showed that the testicular metabolic profiles of ob/ob mice were similar to those of 5'‐AMP treated mice. Exogenous 5'‐AMP inhibited the phosphorylation of AKT and mammalian target of rapamycin signal transduction and reduced the proliferating cell nuclear antigen expressions in testes. Our results suggest that the accumulation of adenosine causes metabolic disorders in testes and associates lower testosterone level in obese mice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The rate of infertility has recently risen as has the alarming increase in obesity (Petraglia, Serour, & Chapron, 2013; Ramaraju et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Adenosine accumulation causes metabolic disorders in testes and associates with lower testosterone level in obese mice

Yang

Zhao

Sun

et al. 2020

Molecular Reproduction Devel

View full text Add to dashboard Cite

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Obesity‐induced oxidative stress and mitochondrial dysfunction negatively affect sperm quality

et al. 2023

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Obesity is a systemic metabolic disease that can induce male infertility or subfertility through oxidative stress. The aim of this study was to determine how obesity impairs sperm mitochondrial structural integrity and function, and reduces sperm quality in both overweight/obese men and mice on a high‐fat diet (HFD). Mice fed the HFD demonstrated higher body weight and increased abdominal fat content than those fed the control diet. Such effects accompanied the decline in antioxidant enzymes, such as glutathione peroxidase (GPX) and catalase and superoxide dismutase (SOD) in testicular and epidydimal tissues. Moreover, malondialdehyde (MDA) content significantly increased in sera. Mature sperm in HFD mice demonstrated higher oxidative stress, including increased mitochondrial reactive oxygen species (ROS) levels and decreased protein expression of GPX1, which may impair mitochondrial structural integrity and reduce mitochondrial membrane potential (MMP) and ATP production. Moreover, cyclic AMPK phosphorylation status increased, whereas sperm motility declined in the HFD mice. Clinical studies demonstrated that being overweight/obese reduced SOD enzyme activity in the seminal plasma and increased ROS in sperm, accompanied by lower MMP and low‐quality sperm. Furthermore, ATP content in the sperm was negatively correlated with increases in the BMI of all clinical subjects. In conclusion, our results suggest that excessive fat intake had similar disruptive effects on sperm mitochondrial structure and function, as well as oxidative stress levels in humans and mice, which in turn induced lower sperm motility. This agreement strengthens the notion that fat‐induced increases in ROS and impaired mitochondrial function contribute to male subfertility.

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Obesity significantly alters the human sperm proteome, with potential implications for fertility

Pini

Parks

Russ

et al. 2020

J Assist Reprod Genet

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Purpose In men, obesity may lead to poor semen parameters and reduced fertility. However, the causative links between obesity and male infertility are not totally clear, particularly on a molecular level. As such, we investigated how obesity modifies the human sperm proteome, to elucidate any important implications for fertility. Methods Sperm protein lysates from 5 men per treatment, classified as a healthy weight (body mass index (BMI) ≤ 25 kg/m 2) or obese (BMI ≥ 30 kg/m 2), were FASP digested, submitted to liquid chromatography tandem mass spectrometry, and compared by label-free quantification. Findings were confirmed for several proteins by qualitative immunofluorescence and a quantitative protein immunoassay. Results A total of 2034 proteins were confidently identified, with 24 proteins being significantly (p < 0.05) less abundant (fold change < 0.05) in the spermatozoa of obese men and 3 being more abundant (fold change > 1.5) compared with healthy weight controls. Proteins with altered abundance were involved in a variety of biological processes, including oxidative stress (GSS, NDUFS2, JAGN1, USP14, ADH5), inflammation (SUGT1, LTA4H), translation (EIF3F, EIF4A2, CSNK1G1), DNA damage repair (UBEA4), and sperm function (NAPA, RNPEP, BANF2). Conclusion These results suggest that oxidative stress and inflammation are closely tied to reproductive dysfunction in obese men. These processes likely impact protein translation and folding during spermatogenesis, leading to poor sperm function and subfertility. The observation of these changes in obese men with no overt andrological diagnosis further suggests that traditional clinical semen assessments fail to detect important biochemical changes in spermatozoa which may compromise fertility.

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Association between obesity and sperm quality

Cited by 63 publications

References 91 publications

Adenosine accumulation causes metabolic disorders in testes and associates with lower testosterone level in obese mice

Adenosine accumulation causes metabolic disorders in testes and associates with lower testosterone level in obese mice

Obesity‐induced oxidative stress and mitochondrial dysfunction negatively affect sperm quality

Obesity significantly alters the human sperm proteome, with potential implications for fertility

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