Association between Mycobacterium avium subsp. paratuberculosis DNA in blood and cellular and humoral immune response in inflammatory bowel disease patients and controls

Juste, Ramón A.; Elguezabal, Natalia; Pavón, Andrés; Garrido, Joseba M.; Geijó, Mariví; Sevilla, Iker A.; Cabriada, José Luis; Tejada, Ángel Morales-Martínez de; García-Campos, Francisco; Casado, Roberto; Ochotorena, Itziar; Izeta, Ander

doi:10.1016/j.ijid.2008.06.034

Cited by 62 publications

(41 citation statements)

References 26 publications

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“…It is currently recognized as one of the main diseases of dairy cattle in industrialized countries and results in substantial financial losses every year for the dairy industry (Hasonova and Pavlik, 2006). Beyond this financial damage, bovine MAP infection may pose a public health risk, as MAP has been linked to Crohn's disease in humans (Juste et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Identification of single nucleotide polymorphisms in the bovine solute carrier family 11 member 1 (SLC11A1) gene and their association with infection by Mycobacterium avium subspecies paratuberculosis

Ruiz-Larrañaga

Garrido

Manzano

et al. 2010

Journal of Dairy Science

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Johne's disease is a chronic enteritis caused by Mycobacterium avium ssp. paratuberculosis (MAP) that causes substantial financial losses for the cattle industry. Susceptibility to MAP infection is reported to be determined in part by genetic factors, so markerassisted selection could help to obtain bovine populations that are increasingly resistant to MAP infection. Solute carrier family 11 member 1 (SLC11A1) was adjudged to be a potential candidate gene because of its role in innate immunity, its involvement in susceptibility to numerous intracellular infections, and its previous association with bovine MAP infection. The objectives of this study were to carry out an exhaustive process of discovery and compilation of polymorphisms in SLC11A1 gene, and to perform a population-based genetic association study to test its implication in susceptibility to MAP infection in cattle. In all, 57 single nucleotide polymorphisms (SNP) were detected, 25 of which are newly described in Bos taurus. Twenty-four SNP and two 3′-untranslated region polymorphisms, previously analyzed, were selected for a subsequent association study in 558 European Holstein-Friesian animals. The SNP c.1067C > G and c.1157-91A > T and a haplotype formed by these 2 SNP yielded significant association with susceptibility to MAP infection. The c.1067C > G is a nonsynonymous SNP that causes an amino acid change in codon 356 from proline to alanine (P356A) that could alter SLC11A1 protein function. This association study supports the involvement of SLC11A1 gene in susceptibility to MAP infection in cattle. Our results suggest that SNP c.1067C > G may be a potential causal variant, although functional studies are needed to assure this point.

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Section: Introductionmentioning

confidence: 99%

Identification of single nucleotide polymorphisms in the bovine solute carrier family 11 member 1 (SLC11A1) gene and their association with infection by Mycobacterium avium subspecies paratuberculosis

Ruiz-Larrañaga

Garrido

Manzano

et al. 2010

Journal of Dairy Science

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“…In 2009 Juste et al from Spain, employed the same methods, nested PCR for IS900, on buffy coat, and reported 15% of CD patients, 19/124, to be positive, 19% of UC patients, 17/90, and 43%, 34/80, of healthy controls. 59 Kirkwood et al tested both peripheral blood mononuclear cells (PBMC) and fresh biopsies, using a similar nested PCR for IS 900. 20 These were from pediatric cases of CD that had never been treated.…”

Section: Molecular Methodsmentioning

confidence: 99%

Where are the weapons of mass destruction — the Mycobacterium paratuberculosis in Crohn's disease?

Kruiningen¹

2011

Journal of Crohn's and Colitis

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“…The nested PCR technique has proved to be popular and accurate, with recent studies using this method to identify MAP in IBD patients more frequently than in control patients. 77,78 Another recent study using the nested IS900 PCR method demonstrated the presence of MAP DNA in children with CD at early onset, 79 which is the earliest identification of MAP DNA in a patient to date. There have now been several studies that have shown the presence of MAP DNA in adult blood samples 73,80,81 and in diseased tissue.…”

Section: Mycobacterium Avium Subspecies Para-tuberculosis and Crohn'smentioning

confidence: 99%

The Role of Bacteria in the Pathogenesis of Inflammatory Bowel Disease

Friswell¹,

Campbell²,

Rhodes³

2010

Gut Liver

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Crohn's disease (CD) and ulcerative colitis (UC) have features that suggest bacterial involvement, and all genetic models of inflammatory bowel disease (IBD) require the presence of commensal bacteria. CD is associated with innate immune response genes such as NOD2/CARD15 and the autophagy genes ATG16L1 and IRGM. However, IBD responds to immunosuppression, suggesting that any bacteria involved are not acting as conventional pathogens. Molecular techniques are rapidly advancing our knowledge of the gut microbiota. In CD there is reduced diversity, and notably a reduction in the probiotic Faecalibacterium prausnitzii, the presence of which in the terminal ileum is associated with a reduced risk of recurrence following surgery. There is also a consistent increase in mucosa-associated Escherichia coli with an "adherent and invasive" phenotype, which allows them to replicate inside macrophages and induce granulomas. Speculation that CD could be caused by the Mycobacterium avium subspecies paratuberculosis (MAP) continues. The response to antitumor necrosis factor treatments suggests that, if relevant at all, MAP is not acting as a conventional pathogen. However, there is increased colonization by MAP in CD, and there is evidence that it could have an indirect effect mediated by the suppression of macrophage function. UC relapse is frequently associated with infection by pathogens, but there is less evidence for involvement of a specific bacterial species. Poor barrier integrity followed by an inflammatory reaction to bacterial components, with chronicity maintained by an autoimmune process, seems a plausible pathogenic model. Bacterial theories of pathogenesis are now becoming testable by targeted therapeutic interventions.

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Association between Mycobacterium avium subsp. paratuberculosis DNA in blood and cellular and humoral immune response in inflammatory bowel disease patients and controls

Abstract: These results indicate the existence of immune responses and treatment interactions with MAP that strongly support an etiological role of this agent in IBD.

Cited by 62 publications

References 26 publications

Identification of single nucleotide polymorphisms in the bovine solute carrier family 11 member 1 (SLC11A1) gene and their association with infection by Mycobacterium avium subspecies paratuberculosis

Identification of single nucleotide polymorphisms in the bovine solute carrier family 11 member 1 (SLC11A1) gene and their association with infection by Mycobacterium avium subspecies paratuberculosis

Where are the weapons of mass destruction — the Mycobacterium paratuberculosis in Crohn's disease?

The Role of Bacteria in the Pathogenesis of Inflammatory Bowel Disease

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