Association between metabolic profiles in urine and bone mineral density of pre- and postmenopausal Chinese women

Yu, Lisha; Qi, Huanhuan; An, Guohua; Bao, Jun; Ma, Bo; Zhu, Jianwei; Ouyang, Gang; Zhang, Pengling; Fan, Hongwei; Zhang, Qi

doi:10.1097/gme.0000000000001158

Cited by 21 publications

(25 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The taurine metabolic pathway was associated with bone demineralization in premenopausal and postmenopausal Chinese women. Thus, reduced taurine is a potential biomarker of osteoporosis [50]. The results of correlation between taurine and the marker of bone formation and resorption showed that the expression of taurine was positive to PINP and negative to CTX-1 and TRACP; the Pearson correlation coefficients were 0.46, −0.59, and −0.57, respectively (Figure 5).…”

Section: Discussionmentioning

confidence: 95%

Urinary Metabolic Profiling via LC-MS/MS Reveals Impact of Bovine Lactoferrin on Bone Formation in Growing SD Rats

Zhao

Ren

et al. 2020

Nutrients

View full text Add to dashboard Cite

Lactoferrin (LF) exerts a promoting bone health function. The effects of LF on bone formation at the metabolic level have been less explored. Urinary metabolic profiling of growing Sprague-Dawley (SD) rats LF-supplemented (1000 mg/kg bw) for four weeks were explored by Liquid chromatography–tandem mass spectrometry (LC-MS/MS). The serum markers of bone formation and bone resorption, the bone mass, and the osteogenesis markers of femur were measured by an enzyme-linked immunosorbent assay, micro-computerized tomography, and immunohistochemistry, respectively. Compared with the control, LF supplementation improved bone formation (p < 0.05), reduced bone resorption (p < 0.05), enhanced femoral bone mineral density and microarchitecture (p < 0.05), and upregulated osteocalcin, osterix, and Runx-2 expression (p < 0.05) of femur. LF upregulated 69 urinary metabolites. KEGG and pathway enrichment analyses of those urinary metabolites, and the Person’s correlation analyses among those urinary metabolites and bone status revealed that LF impacted on bone formation via regulatory comprehensive pathways including taurine and hypotaurine metabolism, arginine and proline metabolism, cyanoamino acid metabolism, nitrogen metabolism, nicotinate and nicotinamide metabolism, and fatty acid biosynthesis. The present study indicated the metabolomics is a useful and practical tool to elucidate the mechanisms by which LF augments bone mass formation in growing animals.

show abstract

Section: Discussionmentioning

confidence: 95%

Urinary Metabolic Profiling via LC-MS/MS Reveals Impact of Bovine Lactoferrin on Bone Formation in Growing SD Rats

Zhao

Ren

et al. 2020

Nutrients

View full text Add to dashboard Cite

show abstract

“…They identified significant alterations in four metabolites: lactate, acetone, acetate, and glutamine [ 19 ]. Subsequently, several metabolomics studies have highlighted the importance of amino-acid metabolism, lipid metabolism, and bile-acid biosynthesis concerning bone health [ 23 , 24 , 35 , 36 , 37 , 38 ]. This is the first metabolomics study of LBMD that considers and excludes the effects of several confounding factors that affect bone density to the best of our knowledge.…”

Section: Discussionmentioning

confidence: 99%

“…These findings are consistent with several previous metabolomics studies that emphasized the potential roles of amino-acid, carbohydrate, nucleoside, lipid, and FA metabolism in bone health [ 23 , 24 , 36 ]. In addition, urinary metabolomics profile analysis of pre-and postmenopausal OP indicated that amino-acid metabolism (such as taurine and β-alanine) and energy metabolism pathways were related to LBMD [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

A Distinctive Human Metabolomics Alteration Associated with Osteopenic and Osteoporotic Patients

et al. 2021

View full text Add to dashboard Cite

Osteoporosis is a common progressive metabolic bone disease resulting in decreased bone mineral density (BMD) and a subsequent increase in fracture risk. The known bone markers are not sensitive and specific enough to reflect the balance in the bone metabolism. Finding a metabolomics-based biomarker specific for bone desorption or lack of bone formation is crucial for predicting bone health earlier. This study aimed to investigate patients’ metabolomic profiles with low BMD (LBMD), including those with osteopenia (ON) and osteoporosis (OP), compared to healthy controls. An untargeted mass spectrometry (MS)-based metabolomics approach was used to analyze serum samples. Results showed a clear separation between patients with LBMD and control (Q2 = 0.986, R2 = 0.994), reflecting a significant difference in the dynamic of metabolic processes between the study groups. A total of 116 putatively identified metabolites were significantly associated with LBMD. Ninety-four metabolites were dysregulated, with 52 up- and 42 downregulated in patients with LBMD compared to controls. Histidine metabolism, aminoacyl-tRNA biosynthesis, glyoxylate, dicarboxylate metabolism, and biosynthesis of unsaturated fatty acids were the most common metabolic pathways dysregulated in LBMD. Furthermore, 35 metabolites were significantly dysregulated between ON and OP groups, with 11 up- and 24 downregulated in ON compared to OP. Among the upregulated metabolites were 3-carboxy-4-methyl-5-propyl-2-2furanopropionic acid (CMPF) and carnitine derivatives (i.e., 3-hydroxy-11-octadecenoylcarnitine, and l-acetylcarnitine), whereas phosphatidylcholine (PC), sphingomyelin (SM), and palmitic acid (PA) were among the downregulated metabolites in ON compared to OP. This study would add a layer to understanding the possible metabolic alterations associated with ON and OP. Additionally, this identified metabolic panel would help develop a prediction model for bone health and OP progression.

show abstract

“…In line with the findings of animal studies, human studies also highlight the importance of amino acid and lipid metabolism in bone. In particular, many amino acids were reported to be associated with BMD by multiple independent studies [105][106][107][108][109] . For example, glutamine is increased 107,110 and proline is decreased in menopausal women with decreased BMD 109 .…”

Section: Metabolomicsmentioning

confidence: 99%

“…For example, glutamine is increased 107,110 and proline is decreased in menopausal women with decreased BMD 109 . Moreover, carbohydrate 108 and nucleoside 106 metabolism are also altered in women with decreased BMD. Finally, BMD-related metabolic pathways have been reported, including bile acid biosynthesis, which might affect intestinal calcium absorption 107 , and the tricarboxylic acid cycle, which might be related to increased oxidative stress in patients with low BMD 108 .…”

Section: Metabolomicsmentioning

confidence: 99%

A road map for understanding molecular and genetic determinants of osteoporosis

et al. 2019

View full text Add to dashboard Cite

Osteoporosis is a highly prevalent disorder characterized by low bone mineral density and an increased risk of fracture, termed osteoporotic fracture. Notably, bone mineral density, osteoporosis and osteoporotic fracture are highly heritable; however, determining the genetic architecture, and especially the underlying genomic and molecular mechanisms, of osteoporosis in vivo in humans is still challenging. In addition to susceptibility loci identified in genome-wide association studies, advances in various omics technologies, including genomics, transcriptomics, epigenomics, proteomics and metabolomics, have all been applied to dissect the pathogenesis of osteoporosis. However, each technology individually cannot capture the entire view of the disease pathology and thus fails to comprehensively identify the underlying pathological molecular mechanisms, especially the regulatory and signalling mechanisms. A change to the status quo calls for integrative multi-omics and inter-omics analyses with approaches in 'systems genetics and

show abstract

Association between metabolic profiles in urine and bone mineral density of pre- and postmenopausal Chinese women

Cited by 21 publications

References 28 publications

Urinary Metabolic Profiling via LC-MS/MS Reveals Impact of Bovine Lactoferrin on Bone Formation in Growing SD Rats

Urinary Metabolic Profiling via LC-MS/MS Reveals Impact of Bovine Lactoferrin on Bone Formation in Growing SD Rats

A Distinctive Human Metabolomics Alteration Associated with Osteopenic and Osteoporotic Patients

A road map for understanding molecular and genetic determinants of osteoporosis

Contact Info

Product

Resources

About