Association between interleukin-6 gene polymorphism and iron regulation in hemodialysis patients infected with HCV

Abstract

Backgrounds: Hepcidin is related to the pathogenesis of chronic renal failure anemia, which is considered a chronic inflammatory state as well as HCV infection. IL-6 stimulates the release of hepcidin from the liver, suppresses intestinal iron uptake, and releases iron from internal stores. Method: To detect the association between IL-6 gene polymorphism and anemia markers, 80 hemodialysis (HD) patients [40 negative HCV HD patients and 40 positive HCV HD patients] were studied by routine chemistry and complete blood count, in addition to the assessment of serum hepcidin, iron parameters [serum iron and serum ferritin], and hepatitis C markers. IL-6 polymorphism -174G/C was determined by MS-PCR, while IL-6 polymorphisms -597G/A and -572 G/C were detected by PCR-SSP. Results: Hepcidin was non-significantly elevated in HCV-positive compared with HCV-negative hemodialysis patients. A statistically significant difference was detected between the negative and positive HCV HD patients in frequencies of IL-6 -174 G/C and -597 G/A (P≤ 0.01 and P≤ 0.001, respectively). On the other hand, a non-significant difference was reported between negative and positive HCV HD patients in the frequencies of IL-6 -572 G/C. Conclusions: Our study indicated that IL-6 -174 G/C and -597 G/A polymorphisms may play a role in HCV susceptibility in HD patients. Additional prospective studies on a larger population are needed to confirm our findings.

Impact of TNFRSF1B (rs3397, rs1061624 and rs1061622) and IL6 (rs1800796, rs1800797 and rs1554606) Gene Polymorphisms on Inflammatory Response in Patients with End-Stage Kidney Disease Undergoing Dialysis

“…IL6 rs1800796 SNP was associated with kidney function and CKD prevalence in a large Japanese population [ 19 ]. Ali et al reported that the IL6 −597 G/A (rs1800797) as well as the −174 (rs1800795) G/C polymorphisms may play a role in susceptibility to hepatitis C virus infection among Egyptian hemodialysis patients [ 40 ]. A meta-analysis revealed that IL6 rs1800795, rs1800796, and rs1800797 SNPs are important players in diabetic nephropathy development [ 41 ].…”

Determination of IL-6 Gene Promoter Polymorphism in Patients with Hepatitis C and Its Impact on RNA Secondary Structure

“…Certain polymorphisms influence interleukin-6 levels [ 6 ]. The IL-6 production levels have been controlled precisely at the transcriptional and translational levels [ 7 ]. The IL-6 gene polymorphisms are designated to consist of regulation at the transcriptional stage, e.g., the (−174G/C) polymorphism [ 8 ], the (−597G/A) polymorphism, and the (−572G/C) polymorphism.…”