Association between interleukin-10 gene polymorphisms (rs1800871, rs1800872, and rs1800896) and severity of infection in different SARS-CoV-2 variants

Abstract

Background Polymorphisms in the interleukin-10 (IL10) gene have been linked to the severity of the patients infected with the viral infections. This study aimed to assess if the IL10 gene polymorphisms rs1800871, rs1800872, and rs1800896 were linked to coronavirus disease 19 (COVID-19) mortality in different severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in the Iranian population. Methods For genotyping IL10 rs1800871, rs1800872, and rs1800896, this study used the polymerase chain reaction-restriction fragment length polymorphism method in 1,734 recovered and 1,450 deceased patients. Results The obtained finding indicated IL10 rs1800871 CC genotype in the Alpha variant and CT genotype in the Delta variant had a relationship with COVID-19 mortality; however, there was no association between rs1800871 polymorphism and the Omicron BA.5 variant. The COVID-19 mortality rate was associated with IL10 rs1800872 TT genotype in the Alpha and Omicron BA.5 variants and GT in the Alpha and Delta variants. The COVID-19 mortality rate was associated with IL10 rs1800896 GG and AG genotypes in the Delta and Omicron BA.5; nevertheless, there was no association between rs1800896 polymorphism with the Alpha variant. According to the obtained data, the GTA haplotype was the most common of haplotype in different SARS-CoV-2 variants. The TCG haplotype was related to COVID-19 mortality in the Alpha, Delta and Omicron BA.5 variants. Conclusion The IL10 polymorphisms had an impact on COVID-19 infection, and these polymorphisms had different effects in various SARS-CoV-2 variants. To verify the obtained results, further studies should be conducted on various ethnic groups.

Association of IL-10–592 C > A /-1082 A > G and the TNFα -308 G > A with susceptibility to COVID-19 and clinical outcomes

“…Our data is the first to unravel the association between polymorphisms in IL-10 gene promoter and COVID-19 in an Egyptian population, which gives insight into the genetic diversity of immune responses towards SARS-COV2. We provided complementary evidence to the role of TNFa-308 polymorphism in a different Egyptian population [ 20 ] and were consistent with findings in Iranian population [ 50 ]. In conclusion, this study sheds light on the genetic determinants influencireatment response, and clinical outcomes in SARS-CoV-2 infection.…”

COVID-19: Focusing on the Link between Inflammation, Vitamin D, MAPK Pathway and Oxidative Stress Genetics

“…Concerning this issue, a previous study reported that polymorphisms in the IL10 gene are linked to the severity of the infected patients. This study found that IL10 gene polymorphisms rs1800871, rs1800872 and rs1800896 were linked to COVID-19 mortality in different SARS-CoV-2 variants in the Iranian population [41].…”

Inflammatory Polymorphisms (IL-6 rs1800796, IL-10 rs1800896, TNF-α rs1800629, and IFITM3 rs12252) Are Not Associated with Post-COVID Symptoms in Previously Hospitalized COVID-19 Survivors

“…There is evidence supporting the role of inflammation in the acute COVID-19 phase [ 4 , 5 ] and also in the post-COVID phase [ 20 ]. Thus, several polymorphisms associated with inflammation have been related to severe COVID-19 illness in previous studies [ 7 , 8 , 9 , 10 ]. Our study did not find an association between four SNPs associated with severe COVID-19, e.g., IL-6 rs1800796 , IL-10 rs1800896 , TNF-α rs1800629 , and IFITM3 rs12252 , and the presence of long-lasting post-COVID symptoms more than one year after infection.…”

“…Different polymorphisms can account for the variability in expression of these pro-inflammatory cytokines [ 6 ]. For instance, genotyping of cytokine-related single-nucleotide polymorphisms (SNPs), e.g., IL-6 rs1800796 [ 7 ], IL-10 rs1800896 [ 8 ], TNF-α rs1800629 [ 9 ], and IFITM3 rs12252 [ 10 ], has shown them to underlie the differential viral virulence and severity of COVID-19. Looking at the research, most studies have investigated the potential role of SNPs at the acute phase of SARS-CoV-2 infection, focusing on the risk of developing the severe form of the COVID-19 illness [ 6 , 7 , 8 , 9 , 10 ].…”

“…For instance, genotyping of cytokine-related single-nucleotide polymorphisms (SNPs), e.g., IL-6 rs1800796 [ 7 ], IL-10 rs1800896 [ 8 ], TNF-α rs1800629 [ 9 ], and IFITM3 rs12252 [ 10 ], has shown them to underlie the differential viral virulence and severity of COVID-19. Looking at the research, most studies have investigated the potential role of SNPs at the acute phase of SARS-CoV-2 infection, focusing on the risk of developing the severe form of the COVID-19 illness [ 6 , 7 , 8 , 9 , 10 ]. Our understanding of the role of these SNPs in symptoms persisting after the acute phase of SARS-CoV-2 infection is still in its infancy.…”