Association between Helicobacter pylori Virulence Factors and Gastroduodenal Diseases in Okinawa, Japan

Matsunari, Osamu; Shiota, Seiji; Suzuki, Rumiko; Watada, Masahide; Kinjo, Nagisa; Murakami, Kazunari; Fujioka, Toshio; Kinjo, Fukunori; Yamaoka, Yoshio

doi:10.1128/jcm.05562-11

Cited by 84 publications

(99 citation statements)

References 54 publications

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“…Antral biopsy specimens were obtained for the isolation of H. pylori by standard culturing technique as previously described [7]. H. pylori DNA was extracted from confluent plate cultures xpanded from a single colony by using DNeasy Blood & Tissue Kit ® (Qiagen, Inc., Santa Clarita, CA).…”

Section: Methodsmentioning

confidence: 99%

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Differences in interleukin 8 expression in Helicobacter pylori–infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic

et al. 2015

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The outcomes of Helicobacter pylori infection vary geographically. H. pylori strains, disease presentation, and environments differ markedly in Bhutan and Dominican Republic. The aims were to compare the strains, histology and expression of interleukin (IL)-8 and IL-10 from gastric mucosa from the two countries. H. pylori status was assessed by the combination of rapid urease test, culture and histology. Histology was evaluated using the updated Sydney System and cytokines in gastric biopsies were measured using real-time PCR. There were 138 subjects from Bhutan and 155 from Dominican Republic. The prevalence of H. pylori infection was 65% and 59%, respectively. The genotype of cagA was predominantly East-Asian type in Bhutan vs. Western type in Dominican Republic. Gastritis severity was significantly higher in H. pylori-infected subjects from Bhutan than those from Dominican Republic. IL-8 expression by H. pylori-infection was 5.5-fold increase in Bhutan vs. 3-fold in Dominican Republic (p <0.001); IL-10 expression was similar. IL-8 expression levels among H. pylori-infected cases tended to be positively correlated with polymorphonuclear leucocyte (PMN) and monocyte infiltration (MNC) scores in both countries. IL-8 expression among those with grade 2 and 3 PMN and MNC was significantly higher in Bhutan than in Dominican Republic. The difference in IL-8 expression in two countries is reflected in the different disease pattern between them. Whether the dominant factor is differences in H. pylori virulence, in host-H. pylori-environmental interactions, genetic factors or all remains unclear. However, severity of inflammation appears to be a critical factor in disease pathogenesis. We compared IL-8 mRNA levels between high gastric cancer risk country; Bhutan (mainly East Asian type H. pylori) and lower gastric cancer risk country; Dominican Republic (mainly Western type H. pylori).

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Section: Methodsmentioning

confidence: 99%

“…H. pylori DNA was extracted from confluent plate cultures xpanded from a single colony by using DNeasy Blood & Tissue Kit ® (Qiagen, Inc., Santa Clarita, CA). The cagA status was determined as previously described [7]. …”

Section: Methodsmentioning

confidence: 99%

Differences in interleukin 8 expression in Helicobacter pylori–infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic

et al. 2015

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“…There are data suggesting that within the same geographic area infection with east Asian-type cagA strains is associated with a higher risk of peptic ulcer or gastric cancer than infection with western-type cagA strains [25–27]. For example, data from Okinawa suggested that the pattern of gastritis in those with east Asian type CagA was that of a rapid progression to atrophic gastritis compared with infections with western types of CagA [28]. However, the individuals with these different types of CagA may also differ in terms of other factors unrelated to CagA type, such as when they or their families migrated to Okinawa and diet among others.…”

Section: Cagamentioning

confidence: 99%

Helicobacter pylori virulence and cancer pathogenesis

2014

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Helicobacter pylori is human gastric pathogen that causes chronic and progressive gastric mucosal inflammation and is responsible for the gastric inflammation-associated diseases, gastric cancer and peptic ulcer disease. specific outcomes reflect the interplay between host-, environmental- and bacterial-specific factors. Progress in understanding putative virulence factors in disease pathogenesis has been limited and many false leads have consumed scarce resources. Few in vitro–in vivo correlations or translational applications have proved clinically relevant. Reported virulence factor-related outcomes reflect differences in relative risk of disease rather than specificity for any specific outcome. Studies of individual virulence factor associations have provided conflicting results. Since virulence factors are linked, studies of groups of putative virulence factors are needed to provide clinically useful information. Here, the authors discuss the progress made in understanding the role of H. pylori virulence factors CagA, vacuolating cytotoxin, OipA and DupA in disease pathogenesis and provide suggestions for future studies.

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“…Eastern Asian H. pylori strains have been documented as more pathogenic, correlating with an increased occurrence of gastric carcinoma (among other GI pathologies) in H. pylori -positive patients in East Asia (including the countries of Japan, Korea, China, and Russia) [41,64]. This observed increase in pathogenicity correlates with an increase in CagA phosphosite abundance [7].…”

Section: The Role Of Biogeographymentioning

confidence: 99%

Helicobacter pylori Eradication in Patients with Immune Thrombocytopenic Purpura: A Review and the Role of Biogeography

et al. 2015

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Idiopathic thrombocytopenic purpura (ITP) is typically a diagnosis of exclusion, assigned by clinicians after ruling out other identifiable etiologies. Since a report by Gasbarrini et al. in 1998, an accumulating body of evidence has proposed a pathophysiological link between ITP and chronic Helicobacter pylori (H. pylori) infection. Clinical reports have described a spontaneous resolution of ITP symptoms in about 50% of chronic ITP patients following empirical treatment of H. pylori infection, but response appears to be geography dependent. Studies have also documented that ITP patients in East Asian countries are more likely to express positive antibody titers against H. pylori-specific cytotoxic-associated gene A (CagA), a virulence factor that is associated with an increased risk for gastric diseases including carcinoma. While a definitive mechanism by which H. pylori may induce thrombocytopenia remains elusive, proposed pathways include molecular mimicry of CagA by host autoantibodies against platelet surface glycoproteins, as well as perturbations in the phagocytic activity of monocytes. Traditional treatments of ITP have been largely empirical, involving the use of immunosuppressive agents and immunoglobulin therapy. However, based on the findings of clinical reports emerging over the past 20 years, health organizations around the world increasingly suggest the detection and eradication of H. pylori as a treatment for ITP. Elucidating the exact molecular mechanisms of platelet activation in H. pylori-positive ITP patients, while considering biogeographical differences in response rates, could offer insight into how best to use clinical H. pylori eradication to treat ITP, but will require well-designed studies to confirm the suggested causative relationship between bacterial infection and an autoimmune disease state.

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Association between Helicobacter pylori Virulence Factors and Gastroduodenal Diseases in Okinawa, Japan

Cited by 84 publications

References 54 publications

Differences in interleukin 8 expression in Helicobacter pylori–infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic

Differences in interleukin 8 expression in Helicobacter pylori–infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic

Helicobacter pylori virulence and cancer pathogenesis

Helicobacter pylori Eradication in Patients with Immune Thrombocytopenic Purpura: A Review and the Role of Biogeography

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