Association between Genotypes of Growth Hormone Gene and Semen Quality Traits in Cow Bulls and Buffalo Bulls

Darwish, Samah F.; Scholkamy, T. H.; Badr, M. A.

doi:10.5455/ajvs.221998

Cited by 1 publication

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“…In dairy bulls, Afshari et al (2011) reported that Iranian Holstein bulls carrying VV genotype of GH gene showed the highest concentration of fresh sperm, while the bulls of LL genotype showed the highest percentage of live sperm with the lowest ejaculate volume. Darwish et al (2016) recorded significant and positive associations between the LV genotype of GH gene with the ejaculate volume, percentage of individual motility and post-thawing sperm motility in Egyptian cattle and buffalo bulls. Nikbin et al (2018), with four polymorphic SNPs in caprine FSHβ and LHβ in male goats, detected significant associations of the gonadotropin genes with semen quality traits in terms of progressive motility, abnormality of fresh semen, motility, velocity and viability traits of post-thaw semen (P<0.05).…”

Section: Associations Of Gh Gene With Rabbit Semen Traitsmentioning

confidence: 93%

“…Therefore, it has a relevant role in reproduction, lactation and normal body growth in rabbits (Fontanesi et al, 2008(Fontanesi et al, , 2012Tusell et al, 2012;Amalianingsih and Brahmantiyo, 2014;Abdel-Kafy et al, 2015;Migdal et al, 2019). In other studies on bulls, the GH gene had a significant effect on the libido score, number of semen doses per collection, post-thaw sperm motility, sperm mass activity, live and dead count, individual fresh sperm motility, total morphological abnormality, head abnormality of sperm, sperm concentration, minor and major defects, scrotal circumference and testicular growth after puberty (Yardibi et al, 2009;Afshari et al, 2011;Pal et al, 2014;Darwish et al, 2016;Amiri et al, 2018). The GH gene could therefore be considered as a candidate gene for the identification of molecular markers associated with growth and semen traits in rabbits.…”

Section: Introductionmentioning

confidence: 99%

“…So, the candidate genes approach might offer the marker of choice for selecting superior bucks with high quality semen traits. In farm animals, many studies concerning the use of GH gene as a molecular marker for semen quality traits have been conducted, such as in dairy bulls (Afshari et al, 2011;Pal et al, 2014;Darwish et al, 2016;Amiri et al, 2018), in buffalo bulls (Darwish et al, 2016), in goat bucks (Nikbin et al, 2018) and in boars (Kmieć et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Association of GH gene polymorphism with growth and semen traits in rabbits

et al. 2021

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Although growth hormone (GH) gene mutations are described in several species, the studies concerning their variabilities and associations with economic traits in rabbits are scarce, particularly associations with semen traits. A total of 149 rabbit bucks from five populations (V-line=36, Moshtohor line=28, APRI line=42, cross ½A½M=23, and Gabali=20) were used in the present study to identify polymorphism of c.-78 C>T single nucleotide polymorphism (SNP) of GH gene among these populations and to investigate the association of GH gene polymorphism with body weight (BW), daily weight gain (DG) and semen traits. DNA was extracted from blood samples for genotyping of c.-78 C>T SNP of GH gene based on polymerase chain reaction with the restriction fragment length polymorphism (PCR-RFLP) technique. The genetic diversity of SNP C>T of GH gene was assessed in terms of genotypic and allelic frequencies, effective number of alleles (Ne), observed (Ho) and expected (He) heterozygosity, Hardy-Weinberg equilibrium (HWE), reduction in heterozygosity due to inbreeding (FIS) and polymorphism information content (PIC). Three genotypes of TT, CC and TC of PCR product of 231 bp of GH gene were detected and all the populations were in HWE in terms of GH gene. The highest Ne was obtained for the Moshtohor line (1.978), while the lowest allelic numbers were obtained for V-line (1.715) and Gabali breed (1.800). The highest genotype frequency of GH gene was 0.48 in TT genotype of V-line, 0.21 in CC genotype of Moshtohor line, 0.67 and 0.56 in TC genotype of ½A½M and Gabali rabbits (P<0.05). The highest frequency for C allele was recorded by Moshtohor line (0.45) and the lowest frequency by Gabali (0.32). The genetic diversity scores for GH gene were intermediate (Ho=0.551, He=0.471, PIC=0.358). The values of Ho ranged from 0.444 in V-line to 0.667 in ½A½M cross, while the values of He were 0.425 in V-line and 0.508 in Moshtohor line. The values of PIC were moderate and ranged from 0.332 in V-line to 0.375 in M-line. The highest FIS was observed in Moshtohor line (0.042) and the lowest value was observed in ½A½M cross (–0.413). The CT genotype of GH gene showed the highest and significant values for body weights at 4, 8, 10 and 12 wk (542, 1131, 1465 and 1861 g) and daily gains at intervals of 4-6 and 8-10 wk (23.1 and 26.5 g). Additionally, the CT genotype recorded the highest and significant values for volume of ejaculate (1.1 mL), sperm motility (57.6%), live sperm (85.6%), normal sperm (93.1%) and sperm concentration in semen (611×106/mL), along with the lowest and significant values for dead sperms (14.4%) and abnormal sperms (6.9%).

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Section: Associations Of Gh Gene With Rabbit Semen Traitsmentioning

confidence: 93%