Association between genotype and drug resistance profiles of Mycobacterium tuberculosis strains circulating in China in a national drug resistance survey

Abstract

We describe the population structure of a representative collection of 3,133 Mycobacterium tuberculosis isolates, collected within the framework of a national resistance survey from 2007 in China. Genotyping data indicate that the epidemic strains in China can be divided into seven major complexes, of which 92% belonged to the East Asian (mainly Beijing strains) or the Euro-American lineage. The epidemic Beijing strains in China are closely related to the Beijing B0/W148 strain earlier described in Russia and a large cluster of these strains has spread national wide. The density of Beijing strains is high in the whole of China (average 70%), but the highest prevalence was found North of the Yellow river. The Euro-American lineage consists of three sublineages (sublineage_1, 2, and 3) and is more prevalent in the South. Beijing lineage showed the highest cluster rate of 48% and a significantly higher level of resistance to rifampicin (14%, p<0.001), ethambutol (9%, p = 0.001), and ofloxacin (5%, p = 0.011). Within the Euro-American Lineage, sublineage_3 revealed the highest cluster rate (28%) and presented a significantly elevated level of resistance to streptomycin (44%, p<0.001). Our findings suggest that standardised treatment in this region may have contributed to the successful spread of certain strains: sublineage_3 in the Euro-American lineage may have thrived when streptomycin was used without rifampicin for treatment, while later under DOTS based treatment, in which rifampicin plays a key role, Beijing lineage appears to be spreading.

Resistance of Mycobacterium tuberculosis strains to Rifampicin: A systematic review and meta-analysis

“…And they were identified as the causative agents of tuberculosis in the world. For each families of MTB resistance to rifampicin, the prevalence was analyzed for the selected studies as follows: Beijing nineteen studies [14, 15, 16, 17, 18, 19, 20, 21, 22, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31], LAM ten studies [15, 17, 23, 25, 28, 29, 32, 33, 34, 35], T ten studies [3, 15, 16, 17, 23, 27, 28, 32, 34, 35], EAI eight studies [18, 22, 25, 27, 28, 29, 33, 36], Ural four studies [15, 17, 30, 34] Haarlem five studies [15, 23, 25, 28, 34], CAS four studies [15, 29, 30, 35] and MANU two studies [16, 29]. The proportion of rifampicin resistance varies by the type of regions and strains.…”

<p>Primary Drug-Resistance Pattern and Trend in Elderly Tuberculosis Patients in Shandong, China, from 2004 to 2019</p>

“…The susceptibility to four first-line drugs (rifampicin, isoniazid, streptomycin, and ethambutol) was tested by the proportional method on Löwenstein-Jensen (LJ) culture medium according to the WHO guidelines. 15 The concentration of drugs were as follows: 0.2 μg/mL (isoniazid, INH), 40 μg/mL (rifampin, RFP), 10 μg/mL (streptomycin, SM), 2 μg/mL (ethambutol, EMB). 16…”

Molecular epidemiology of clinical Mycobacterium tuberculosis complex isolates in South Omo, Southern Ethiopia

“…Understanding the molecular epidemiology of TB is important for regional disease control. For instance, distinct strains may be linked to outbreaks [10], high virulence [42], emergency of drug resistance [44], disease progression [43], and can point to the geographic origin of a strain [20,33] as well as identify new lineages [17,28].…”