Association between Genetic Variants on Chromosome 15q25 Locus and Several Nicotine Dependence Traits in Polish Population: A Case-Control Study

Abstract

Tobacco smoking continues to be a leading cause of disease and mortality. Recent research has confirmed the important role of nicotinic acetylcholine receptor (nAChR) gene cluster on chromosome 15q 24-25 in nicotine dependence and smoking. In this study we tested the association of smoking initiation, age at onset of daily smoking, and heaviness of smoking with five single nucleotide polymorphisms (SNPs) within the CHRNA5-CHRNA3-CHRNB4 cluster. The group of 389 adult subjects of European ancestry from the north of Poland, including 212 ever (140 current and 72 former) and 177 never smokers with mean age 49.26, was genotyped for rs16969868, rs1051730, rs588765, rs6495308, and rs578776 polymorphisms. Distributions of genotypes for rs16969868 and rs1051730 were identical so they were analyzed together. Further analysis revealed the association between rs16969868-1051730 (OR = 2.66; 95% CI: 1.30–5.42) and number of cigarettes smoked per day (CPD) with heaviness of nicotine addiction measured by the Fagerström Test for Nicotine Dependence (FTND) (OR = 2.60; 95% CI: 1.24–5.43). No association between these polymorphisms and other phenotypes was found. Similarly, the association between rs588765, rs6495308, rs578776, and analyzed phenotypes was not confirmed. This study provides strong evidence for the role of the CHRNA5-CHRNA3-CHRNB4 cluster in heaviness of nicotine addiction.

Association Between CHRNA3 and CHRNA5 Nicotine Receptor Subunit Gene Variants and Nicotine Dependence in an Isolated Populationof Kashubians in Poland

“…Finally, Li et al reported that the association of rs6495308 with smoking quantity has been confirmed, at both the individual SNP and at the haplotype level, which indicates that this polymorphism influences ND via an unknown mechanism [ 19 ]. In our study, we did not demonstrate an association between this SNP and CPD or FTND, which is consistent with the results obtained from a non-representative sample of the general Polish population [ 49 ]. Two other ND phenotypes studied in our analyses, HSI and TTF, were also not associated with rs6495308.…”

Cross-Sectional Study to Characterise Nicotine Dependence in Central Vietnamese Men

“…28,29 For instance, variations in CHRNA5 (cholinergic receptor nicotinic alpha 5 subunit) have been shown to influence nicotine dependence in Polish individuals. 30,31 Genetic factors also influence the interaction between smoking and disease development. 32,33 An individual's genetic background is a convertible contributing factor for development of high dependence which consequently makes quitting smoking very difficult.…”

Association between variants in nicotinic acetylcholine receptor genes and smoking cessation in a Chinese rural population

“…The reason for this difference is not clear. It is speculated that it might be resulted from the different frequency of C and T alleles of rs578776 among different populations such as Caucasian, African, and Chinese ancestry population …”