Association between gene expression profile and tumor invasion in oral squamous cell carcinoma

Toruner, Gokce; Ülger, Celal; Alkan, M.; Galante, Anthony; Rinaggio, Joseph; Wilk, Randall M.; Tian, Bin; Soteropoulos, Patricia; Hameed, Meera; Schwalb, Marvin N.; Dermody, James

doi:10.1016/j.cancergencyto.2004.01.026

Cited by 147 publications

(139 citation statements)

References 36 publications

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“…Hundreds of ISG proteins have been previously determined using genomics and proteomics approaches (54,55). Our current study showed that 10 of the 53 up-regulated candidate proteins belong to the ISG family (Table I), and increased mRNA or protein levels of seven proteins (SYWC, IFM1, STAT1, TYPH, UCRP, MX1, and GBP2) have been detected in OSCC via previous genomics or proteomics studies (9,10,70). Of these up-regulated ISG proteins, four (STAT1, UCRP, GBP1, and TYPH) were confirmed to be overexpressed in OSCC tissues via immunohis- tochemical staining (in at least eight of the 10 tissue pairs examined) (Fig.…”

Section: Discussionsupporting

confidence: 57%

Enhanced Interferon Signaling Pathway in Oral Cancer Revealed by Quantitative Proteome Analysis of Microdissected Specimens Using 16O/18O Labeling and Integrated Two-dimensional LC-ESI-MALDI Tandem MS

Chi

Lee

Chang

et al. 2009

Molecular & Cellular Proteomics

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Section: Discussionsupporting

confidence: 57%

Enhanced Interferon Signaling Pathway in Oral Cancer Revealed by Quantitative Proteome Analysis of Microdissected Specimens Using 16O/18O Labeling and Integrated Two-dimensional LC-ESI-MALDI Tandem MS

Chi

Lee

Chang

et al. 2009

Molecular & Cellular Proteomics

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“…It was of interest that HOP was downregulated not only in a panel of lung cancer cell lines tested but also in primary lung tumors. 3 Given the fact that HOP locates on chromosome 4q12, a region found to be frequently deleted in lung cancer, and its potential tumor suppressive role in choriocarcinoma 6 and head and neck carcinoma, 7,8 we focused on the function of HOP in lung cancer.…”

Section: Discussionmentioning

confidence: 99%

“…6 In head and neck carcinomas, decreased expression of HOP was found compared to corresponding normal tissues. 7,8 In thyroid carcinoma; however, the HOP gene was found to be overexpressed compared to normal thyroid tissues suggesting that it could be a potential prooncogene in this kind of cancer. 9 Recently, a study from Yin et al showed that Hop participates in the development of pulmonary airway with evidence that loss of Hop expression in vivo results in defective Type 2 pneumocyte development with increased surfactant production and disrupted alveolar formation.…”

mentioning

confidence: 99%

Homeobox gene HOP has a potential tumor suppressive activity in human lung cancer

Chen

Pacyna-Gengelbach

Deutschmann

et al. 2007

Intl Journal of Cancer

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The homeobox containing gene HOP (Homeodomain Only Protein) was identified in the developing heart and lung where it functions downstream of Nkx2.5 and Nkx2.1 to modulate cardiac and lung gene expression. Previously, we found that HOP was downregulated in lung cancer. In this study, we constructed an expression vector containing the full-length cDNA of HOP and transfected it into a lung cancer cell line H2170. Stable transfection led to an increased expression of HOP confirmed by Northern blot analysis. HOP positive transfectants remarkably reduced the growth rate and the ability of anchorage-independent growth in soft agar, and moreover suppressed the tumor formation in nude mice compared to controls. Transient transfection of Nkx2.1 into H2170 resulted in the overexpression of HOP, and correspondingly, siRNA silencing of Nkx2.1 reduced the expression of HOP in lung cancer cells. Treatment with a differentiation modulating agent 5-bromodeoxyuridine (BrdU) led to restoration of HOP expression in a small cell lung cancer cell line H526. In 29 paired primary lung tumor samples, loss of heterozygosity (LOH) analysis was performed by using the 3 microsatellite markers D4S189, D4S231 and D4S392 around the region of chromosome 4q12 where HOP locates. LOH was only found in 4 out 23 cases (17.4%) indicating that allelic loss is a rare genetic event not responsible for the downregulation of HOP in lung cancer. Taken together, our data suggest that HOP is a potential tumor suppressor possibly involved in lung cancer differentiation, and functions downstream of Nkx2.1. ' 2007 Wiley-Liss, Inc.Key words: HOP; lung cancer; stable transfection; tumor suppressor; RNA interference; differentiation Lung cancer is a predominant cause of cancer death in both men and women. 1 Although current therapies for patients with lung cancer include surgical resection and/or radiotherapy or chemotherapy, the outcome is poor with an overall 5-year survival rate of 15%. New molecular targets for the diagnosis and therapy are therefore required to improve the prognosis for lung cancer patients.In a previous study, we performed suppression subtractive hybridization (SSH) to reveal lung cancer associated genes by comparing the gene expression between normal human bronchial epithelial cells (HBEC) and lung squamous carcinoma cells of H2170. 2 Two cDNA libraries representing mainly the genes that are over-expressed and under-expressed in HBEC and tumor cell line were cloned. The homeobox gene LAGY (Lung Cancer Associated Gene Y; Genbank Accession Nr. AF454763) was identified to be downregulated in lung cancer cell lines compared to HBEC, and a decreased gene expression was also observed in primary lung tumors. 3 LAGY, also called HOP (Homeodomain Only Protein), Cameo (Cardiac homeodomain) and NECC1 (Not Expressed in Choriocarcinoma Clone 1), was initially identified in the embryonic heart and it acts at multiple steps in the pathway for heart development. 4,5 Later, reports about the involvement of this gene in carcinogenesis emerged. For example, t...

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“…7,8 Upregulation of MMP1 appears to a conserved in squamous cell carcinomas from different anatomic locations including the head and neck and oral cavity. [67][68][69][70] Matrix metallopeptidases appear integral to tumorigenesis and have been targeted by inhibitors; however, clinical trials of advanced lung, prostate, pancreas, brain, and GI tract malignancies have shown little efficacy to date. 64,71 More data may be needed before a successful clinical trial is completed.…”

Section: P53 Signalingmentioning

confidence: 99%

Molecular discrimination of cutaneous squamous cell carcinoma from actinic keratosis and normal skin

Hui

Binder

2011

Modern Pathology

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Actinic keratosis is widely believed to be a neoplastic lesion and a precursor to invasive squamous cell carcinoma. However, there has been some debate as to whether actinic keratosis is in fact actually squamous cell carcinoma and should be treated as such. As the clinical management and prognosis of patients is widely held to be different for each of these lesions, our goal was to identify unique gene signatures using DNA microarrays to discriminate among normal skin, actinic keratosis, and squamous cell carcinoma, and examine the molecular pathways of carcinogenesis involved in the progression from normal skin to squamous cell carcinoma. Formalin-fixed and paraffin-embedded blocks of skin: five normal skins (pooled), six actinic keratoses, and six squamous cell carcinomas were retrieved. The RNA was extracted and amplified. The labeled targets were hybridized to the Affymetrix human U133plus2.0 array and the acquisition and initial quantification of array images were performed using the GCOS (Affymetrix). The subsequent data analyses were performed using DNA-Chip Analyzer and Partek Genomic Suite 6.4. Significant differential gene expression (42 fold change, Po0.05) was seen with 382 differentially expressed genes between squamous cell carcinoma and normal skin, 423 differentially expressed genes between actinic keratosis and normal skin, and 9 differentially expressed genes between actinic keratosis and squamous cell carcinoma. The differentially expressed genes offer the possibility of using DNA microarrays as a molecular diagnostic tool to distinguish between normal skin, actinic keratosis, and squamous cell carcinoma. In addition, the differentially expressed genes and their molecular pathways could be potentially used as prognostic markers or targets for future therapeutic innovations.

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Association between gene expression profile and tumor invasion in oral squamous cell carcinoma

Cited by 147 publications

References 36 publications

Enhanced Interferon Signaling Pathway in Oral Cancer Revealed by Quantitative Proteome Analysis of Microdissected Specimens Using 16O/18O Labeling and Integrated Two-dimensional LC-ESI-MALDI Tandem MS

Enhanced Interferon Signaling Pathway in Oral Cancer Revealed by Quantitative Proteome Analysis of Microdissected Specimens Using 16O/18O Labeling and Integrated Two-dimensional LC-ESI-MALDI Tandem MS

Homeobox gene HOP has a potential tumor suppressive activity in human lung cancer

Molecular discrimination of cutaneous squamous cell carcinoma from actinic keratosis and normal skin

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