Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle

Hoashi, Shogo; Hinenoya, Tomoko; Tanaka, Atsuko; Ohsaki, Hideki; Sasazaki, Shinji; Taniguchi, Masaaki; Oyama, Kenji; Mukai, Fumio; Mannen, Hideyuki

doi:10.1186/1471-2156-9-84

Cited by 100 publications

(87 citation statements)

References 27 publications

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“…Interestingly, in the livestock field, it is known as a key candidate gene for meat marbling, which is a phenomenon quite similar to muscle fatty infiltration 30, 31. Genetic ablation and pharmacological inhibition studies have shown that FABP4 plays a critical role in many aspects of the development of metabolic diseases and have suggested FABP4 as a therapeutic target for such diseases 29, 32, 33, 34.…”

Section: Introductionmentioning

confidence: 99%

Fatty acid‐binding protein 4 regulates fatty infiltration after rotator cuff tear by hypoxia‐inducible factor 1 in mice

Lee

Kim

et al. 2017

J cachexia sarcopenia muscle

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BackgroundFatty infiltration in skeletal muscle is directly linked to loss of muscle strength and is associated with various adverse physical outcomes such as muscle atrophy, inflammation, insulin resistance, mobility impairments, and even mortality in the elderly. Aging, mechanical unloading, muscle injury, and hormonal imbalance are main causes of muscle fat accumulation, and the fat cells are derived from muscle stem cells via adipogenic differentiation. However, the pathogenesis and molecular mechanisms of fatty infiltration in muscles are still not fully defined. Fatty acid‐binding protein 4 (FABP4) is a carrier protein for fatty acids and is involved in fatty acid uptake, transport, and lipid metabolism. Rotator cuff tear (RCT) usually occurs in the elderly and is closely related with fatty infiltration in injured muscle. To investigate potential mechanisms for fatty infiltration other than adipogenic differentiation of muscle stem cells, we examined the role of FABP4 in muscle fatty infiltration in an RCT mouse model.MethodsIn the RCT model, we evaluated the expression of FABP4 by qRT‐PCR, western blotting, and immunohistochemical analyses. Histological changes such as inflammation and fat accumulation in the injured muscles were examined immunohistochemically. To evaluate whether hypoxia induces FABP4 expression, the levels of FABP4 mRNA and protein in C3H10T1/2 cells after hypoxia were examined. Using a transient transfection assay in 293T cells, we assessed the promoter activity of FABP4 by hypoxia‐inducible factors (HIFs). Additionally, we evaluated the reduction in FABP4 expression and fat accumulation using specific inhibitors for HIF1 and FABP4, respectively.ResultsFABP4 expression was significantly increased after RCT in mice, and its expression was localized in the intramuscular fatty region. Rotator cuff tear‐induced FABP4 expression was up‐regulated by hypoxia. HIF1α, which is activated by hypoxia, augmented the promoter activity of FABP4, together with HIF1β. Hypoxia‐induced FABP4 expression was significantly decreased by HIF1 inhibitor treatment. Furthermore, in RCT model mice, fat accumulation was remarkably reduced by FABP4 inhibitor treatment.ConclusionsThis study shows that RCT induces FABP4 expression, leading to fat accumulation in injured muscle. FABP4 transcription is regulated by the direct binding of HIF1 to the FABP4 promoter in the hypoxic condition induced by RCT. Fat accumulation in injured muscle was reduced by the inhibition of FABP4. Ultimately, in the RCT model, we identified a novel mechanism for fatty infiltration by FABP4, which differs from adipogenic differentiation of muscle stem cells, and we found that fatty infiltration might be regulated by inhibition of HIF1 or FABP4.

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Section: Introductionmentioning

confidence: 99%

Fatty acid‐binding protein 4 regulates fatty infiltration after rotator cuff tear by hypoxia‐inducible factor 1 in mice

Lee

Kim

et al. 2017

J cachexia sarcopenia muscle

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“…In this study, we investigated the single nucleotide polymorphisms (SNPs) of FABP4 gene (the 7516G>C SNP) and SCD gene (SNP 878T>C), which have been reported to have an effect on the fatty acid composition and content in beef and therefore influence its flavour and juiciness (Taniguchi et al 2004;Michal et al 2006;Hoashi et al 2008;Matsuhashi et al 2011). The purpose of this work was to evaluate the relationship between these genes and the FA content in Aberdeen Angus and Blonde d'Aquitaine breeds of beef cattle.…”

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confidence: 99%

Polymorphic effects of FABP4 and SCD genes on intramuscular fatty acid profiles in longissimus muscle from two cattle breeds

et al. 2015

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The aim of this study was to evaluate the polymorphic effects of two single nucleotide polymorphisms (SNPs) of fatty acid binding protein (FABP4) and stearoyl-CoA desaturase (SCD) genes on intramuscular fatty acid profiles in the longissimus muscle in two cattle breeds. Two previously reported SNPs of bovine FABP4 (7516G>C) and SCD (878C>T) were in turn assessed for their associations with intramuscular fatty acid profiles from the upper sirloin cuts of Aberdeen Angus and Blonde d'Aquitaine cattle. In total, 33 animals were genotyped using PCR-RFLP. Intramuscular fatty acid composition was evaluated using two complementary statistical approaches: a classical univariate regression model and a multivariate approach using a combination of Principal Component Analysis and Random Forests. Significant effect of FABP4

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“…The effects of four nonsynonymous substitutions in FABP4 and LXRα were investigated using a Japanese Black cattle population (Hoashi et al, 2008). The FABP4 I74V had a highly significant effect on the percentage of C16:1.…”

Section: Sterol Regulatory Element Binding Protein-1mentioning

confidence: 99%

“…We also surveyed additional genetic markers associated with fatty acid composition (Hoashi et al 2008). The fulllength CDS for six genes, adipocytes fatty acid binding protein (FABP4), liver X receptor α (LXRα), cytochrome b 5 , long-chain acyl-CoA synthetase (ACSL) 1, ACSL4, and diacylglycerol acyltransferase 2, were sequenced to identify polymorphisms in Japanese Black and Holstein cattle.…”

Section: Sterol Regulatory Element Binding Protein-1mentioning

confidence: 99%

Genes Associated with Fatty Acid Composition of Beef

Mannen

2012

FSTR

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Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle

Abstract: Background: Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of six genes, adipocytes-type fatty acid binding

Cited by 100 publications

References 27 publications

Fatty acid‐binding protein 4 regulates fatty infiltration after rotator cuff tear by hypoxia‐inducible factor 1 in mice

Fatty acid‐binding protein 4 regulates fatty infiltration after rotator cuff tear by hypoxia‐inducible factor 1 in mice

Polymorphic effects of FABP4 and SCD genes on intramuscular fatty acid profiles in longissimus muscle from two cattle breeds

Genes Associated with Fatty Acid Composition of Beef

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