Association between FAS polymorphism and prostate cancer development

Abstract

The role of FAS polymorphisms in prostate cancer has not been studied. Using the PCR-based restriction fragment-length polymorphism methodology, we evaluated FAS gene locus À670 genotypes in DNA from 904 men: 657 prostate cancer patients and 247 healthy controls. We found that carriers of AG or GG genotypes have a statistically significant protection (odds ratio (OR) ¼ 0.30; confidence interval (CI): 0.20-0.44 and OR ¼ 0.22; CI: 0.12-0.74, respectively) for disease with extra-capsular invasion. Taken together, a 72% protection was found for G allele carriers (OR ¼ 0.28; CI: 0.19-0.41). Fas exist as membrane-bound and soluble forms and with opposite roles. They derive from the same gene by alternative splicing. Membrane Fas receptors trigger apoptosis whereas, on the other hand, soluble Fas (sFas) bind to Fas ligand antagonizing Fas-Fas ligand apoptotic pathway. Our results suggest that G allele may reduce sFas levels preventing the apoptotic inhibition caused by the soluble form.

IL-4 and TNF-α Polymorphisms Are Associated with Risk of Multiple Superficial Tumors or Carcinoma in situ Development

“…IL4-590C/T polymorphism genotyping was performed with the PCR-RFLP method as previously described [46,48] , and it was successfully amplified in 89.4% of the samples. A second PCR-RFLP analysis was carried out in 10% of all samples for genotype confirmation.…”

“…Peripheral blood samples were collected following a standard venipuncture technique in EDTA-containing tubes, and the DNA was extracted from the white blood cell fraction using a saltingout protocol [46] . The polymorphism analysis was carried out using methodologies published by other authors [47,48] .…”

The Relationship of Fas Promoter Polymorphisms and Breast Cancer Risk in North-West of Iran: A Haplotype and in Silico Analysis

“…To the best of the authors' knowledge, as the first case-control study in northwest of Iran, we investigated the correlation between 2 common polymorphisms in the promoter of fas. The present study did not show any significant association between the risk for breast cancer and fas-670A > G. The results of studies on breast cancer in Chinese population (3) and prostate cancer in Portuguese population (27) revealed that the fas-670GG genotype had a protective effect on the development of breast and prostate cancers. Recently, Xu et al applied a meta-analysis of cancer cases and controls, including fas-1377G > A and fas-670A > G from 52 case-control studies, with similar results to the present study; the results showed no significant association between fas-670A > G and breast cancer risk that might have variability based on various tumor types, examined population, and different technics in genotyping assay.…”

Association analysis of FAS‑670A/G and FASL‑844C/T polymorphisms with risk of generalized aggressive periodontitis disease

“…In general, FASL has been implicated in the induction of T-cell apoptosis (11). Alterations in the regulation of FAS/FASL may contribute to the pathogenesis of various human diseases including cancer and inflammatory diseases such as periodontitis (31).…”