Association between End-stage renal disease and the polymorphism of HLA in Guangxi Zhuang population

Abstract

Objective: To investigate the genetic relationship between end-stage renal disease (ESRD) and the Guangxi Zhuang population's human leukocyte antigen (HLA) allele. Methods: To perform the polymerase chain reaction reversed sequence-specific oligonucleotide (PCR-rSSO)method, genotyping for 325 patients with ESRD at the HLA-A, B, C, DRB1, and DQB1 loci was done. The direct counting method was used to determine the HLA alleles' frequencies, and Arlequin software(3.5.2.2) was used for haplotypic frequency analyses, compared with 350 healthy donors of Guangxi Zhuang nationality. Results: We found that only HLA-DRB1*14:54 showed a positive association with ESRD (P=0.005, Pc=0.035, OR=1.484, CI=1.122-1.963) after Bonferroni correction, so that it may be a protective allele for ESRD. A*11:01-B*15:02-DRB1*15:01 and A*11:01-C*08:01-B*15:02-DRB1*12:02-DQB1*03:01 were more prevalent in ESRD after Bonferroni correction. Conclusion: ESRD patients and the healthy population in Guangxi Zhuang have high HLA-A, B, C, DRB1, and DQB1 allele and haplotype frequencies. DRB1*14:54, A*11:01-B*15:02-DRB1*15:01, and A*11:01-C*08:01-B*15:02-DRB1*12:02-DQB1*03:01 were potentially valuable allele and haplotypes for evaluating the risk of ESRD in Guangxi Zhuang population.