Association between DLK1 and IGF-I gene expression and meat quality in sheep

Su, Rui; Sun, Wei; Li, D; Wang, Q Z; Lv, Xiaoqing; Musa, H H; Chen, L; Zhang, Y F; Wu, Wenbo

doi:10.4238/2014.december.4.26

Cited by 16 publications

(10 citation statements)

References 35 publications

(39 reference statements)

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“…The results indicated that the Barki breed had a significant down-regulation of IGF-1compared to the Ossimi breed in 7-9 months age category and Ossimi and Rahmani breeds in the 10-12 months age category. The result of this study is different than results reported by Su et al (2014) who mentioned that IGF-I expression in Hu sheep increased from 2 days of birth and then continuously decreased until 3 months of age; thereafter, IGF-I expression gradually increased and reached the peak at 6 months of age, the expression of IGF-I in sheep that were 6 months of age was significant (P<0.01) and greater than at 1, 2, 3, and 4 months of age. The IGF1 gene plays an important purpose in the growth of multiple tissues, including mammalian muscle, somatic growth, and tissue repair (Nazari et al, 2016, Grochowska et al, 2017Rotwein, 2017).…”

Section: Gene Expression Analysiscontrasting

confidence: 99%

Evaluation of Growth Performance, Blood Metabolites and Gene Expression Analysis in Egyptian Sheep Breeds, in Relation to Age

Ashour¹,

Gad²,

Fayed³

et al. 2020

jwpr

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The growth performance of lambs attributes the economic viability of animals. Faster growth allows lambs to reach maturity in early age. Therefore, the aim of this study was to compare growth performance, blood metabolites and expression of IGF-1, GH, and Leptin genes in three different Egyptian sheep breeds across age. Thirty Egyptian sheep males from three breeds (Ossimi, Rahmani and Barki) were divided into three ages categorize (7-9, 10-12, and 13-16 months). The results showed that there was a significant increase in sheep's live body weights toward advanced ages till the second age category for all breeds, the highest values of linear body measurements were observed in Ossimi breed. There was a non-significant inverse effect of advanced age on blood glucose and total lipids levels in all sheep breeds. There wasn't any significant effect of interaction between age and breed on plasma total protein concentrations. According to age categories, Barki breed showed a significant up-regulation of GH compared to the Ossimi breed in 7-9 months age category. However, Barki breed showed a significant downregulation of IGF-1compared to the Ossimi breed in 7-9 months. Meanwhile, Leptin expression showed significant differences in Ossimi breed between 10-12 months age category and two other age categories. We concluded that measuring of physical body measurements, blood metabolites and GH, IGF-1 and Leptin genes in early ages is a good and accurate indicator for growth performance in Egyptian sheep breeds.

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Section: Gene Expression Analysiscontrasting

confidence: 99%

Evaluation of Growth Performance, Blood Metabolites and Gene Expression Analysis in Egyptian Sheep Breeds, in Relation to Age

Ashour¹,

Gad²,

Fayed³

et al. 2020

jwpr

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“…This might also be related to the fact that for some of the SNPs, a low representation of alternative homozygotes was found in the studied population. However, associations of segregating SNPs in IGF1 and DGAT1 with growth, milk, and wool performance traits have been described in various sheep breeds such as Makeoi, Baluchi, Hu, Sarda, and Mehraban [55][56][57][58][59][60][61][62]. These breed differences might be due to artificial selection pressures or housing due to geographical conditions.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Candidate Genes for Growth and Milk Performance Traits in the Egyptian Barki Sheep

Abousoliman

Reyer

Oster

et al. 2020

Animals

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The most common sheep breeds of Egypt are Ossimi, Rahmani, and Barki breeds. The latter one is well adapted to the challenging desert environment, characterized by food shortage and a high temperature fluctuation. Growth performance of Barki sheep has an important economic value in terms of minimizing the shortage of mutton meat in Egypt. Further, milk production is of great importance for feeding newborn lambs. Eight candidate genes, recently associated with production traits in different breeds, were used to study the effect of genotype on lamb growth and ewe milk traits. The examined genes were LEP, IGF1, DGAT1, STAT5A, PRL, CSN1S2, GHR, and GHRHR, of which one representative single nucleotide polymorphism (SNP) located in the coding region was selected for genotyping. Data from 251 Barki sheep were used in this study. Association analysis between SNPs and lamb growth traits identified rs420693815 of the LEP gene to be significantly associated with weaning weight and average daily gain. In ewes, significant effects on milk yield and composition have been estimated for LEP (rs420693815), STAT5A (rs161082816), PRL (rs422713690), and GHRHR (rs414991449). The results indicated that these genes might be considered as interesting candidates for further investigations to improve growth and milk performance in Barki sheep.

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“…There is evidence showing that DLK1 may be involved in IGF1 signaling and in muscle growth through Akt phosphorylation (22,(31)(32)(33). We measured Akt phosphorylation in hypertrophic muscles.…”

Section: Significancementioning

confidence: 99%

Regulation of DLK1 by the maternally expressed miR-379/miR-544 cluster may underlie callipyge polar overdominance inheritance

Gao

Chen

Wang

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Inheritance of the callipyge phenotype in sheep is an example of polar overdominance inheritance, an unusual mode of inheritance. To investigate the underlying molecular mechanism, we profiled the expression of the genes located in the Delta-like 1 homolog (Dlk1)-type III iodothyronine deiodinase (Dio3) imprinting region in mice. We found that the transcripts of the microRNA (miR) 379/miR-544 cluster were highly expressed in neonatal muscle and paralleled the expression of the Dlk1. We then determined the in vivo role of the miR-379/miR-544 cluster by establishing a mouse line in which the cluster was ablated. The maternal heterozygotes of young mutant mice displayed a hypertrophic tibialis anterior muscle, extensor digitorum longus muscle, gastrocnemius muscle, and gluteus maximus muscle and elevated expression of the DLK1 protein. Reduced expression of DLK1 was mediated by miR-329, a member of this cluster. Our results suggest that maternal expression of the imprinted miR-379/miR-544 cluster regulates paternal expression of the Dlk1 gene in mice. We therefore propose a miR-based molecular working model for polar overdominance inheritance. muscle hypertrophy | callipyge | DLK1 | miR-379/miR-544 cluster T he callipyge (CLPG) phenotype can be characterized by an increase in muscle mass due to muscular hypertrophy and a unique non-Mendelian mode of inheritance (1-3). CLPG individuals are born with normal muscle histology and develop muscle hypertrophy at ∼1 mo of age. This hypertrophy occurs primarily in the muscles of the pelvic limbs and loin and some muscles in the shoulder, which are enriched with a high proportion of type IIB fast-twitch glycolytic fibers (4). Although the molecular regulation of these hypertrophic processes is yet to be determined, Delta-like 1 homolog (DLK1) is found to be the causative protein (5-10). The unique mode of inheritance of the CLPG phenotype has attracted a great deal of attention within the scientific community. The CLPG offspring inherit the phenotype only when the mutated allele comes from the father and the wild-type allele from the mother (CLPG PAT /+ MAT ). CLPG PAT /CLPG MAT offspring exhibit a wild-type phenotype, despite carrying a CLPG mutation on the paternal chromosome. This unusual mode of inheritance led to the development of the genetic concept of polar overdominance (2, 11).The CLPG mutation has been mapped to the Dlk1-Dio3 imprinted domain, which spans ∼1 Mb and harbors at least three protein-encoding genes, including Dlk1, Peg11/Rtl1, and Dio3, and multiple noncoding RNA genes, including Gtl2, anti-Peg11/anti-Rtl1, Meg8, Mirg, C/D small nucleolar RNAs (snoRNAs), and microRNA clusters [the microRNA (miR) 379 and miR-127 clusters] (12-14). The coding genes are expressed paternally, whereas the noncoding genes are expressed maternally (15,16). Further analysis revealed a point mutation (A to G transition) within the intergenic region between the Dlk1 and Gtl2 genes of the imprinted Dlk1-Dio3 region (17, 18), which affects a muscle-specific, long-range cis-acting ...

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Association between DLK1 and IGF-I gene expression and meat quality in sheep

Cited by 16 publications

References 35 publications

Evaluation of Growth Performance, Blood Metabolites and Gene Expression Analysis in Egyptian Sheep Breeds, in Relation to Age

Evaluation of Growth Performance, Blood Metabolites and Gene Expression Analysis in Egyptian Sheep Breeds, in Relation to Age

Analysis of Candidate Genes for Growth and Milk Performance Traits in the Egyptian Barki Sheep

Regulation of DLK1 by the maternally expressed miR-379/miR-544 cluster may underlie callipyge polar overdominance inheritance

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