Association between Consumption of Cruciferous Vegetables and Condiments and Excretion in Urine of Isothiocyanate Mercapturic Acids

Vermeulen, Martijn; Berg, R. van den; Freidig, Andreas P.; Bladeren, P.J. van; Vaes, Wouter H. J.

doi:10.1021/jf060723n

Cited by 75 publications

(72 citation statements)

References 22 publications

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“…However, these compounds are structurally similar and reveal similar pharmacokinetics. Moreover, interconversion of SFN to ERN has been reported in rats and humans (46,47). Concentration of these ITC in plasma or tissue after multiple dosing of pure compounds can be higher than after 1 dose of plant extract, however it has not been investigated so far.…”

Section: Discussionmentioning

confidence: 99%

Sensitization of HER2 Positive Breast Cancer Cells to Lapatinib Using Plants-Derived Isothiocyanates

Kaczyńska

Swierczynska

Herman-Antosiewicz

2015

Nutrition and Cancer

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Nearly 25% of all breast cancer is characterized by overexpression of HER2 (human epidermal growth factor receptor 2) which leads to overactivation of prosurvival signal transduction pathways, especially through Akt-mTOR-S6K kinases, and results in enhanced proliferation, migration, induction of angiogenesis, and apoptosis inhibition. Anti-HER2 targeted therapies, such as specific monoclonal antibodies or small-molecule tyrosine kinase inhibitors, even in combination, still seem to be insufficient due to incidence of primary or acquired resistance and prevalence of serious side-effects of these drugs. We assumed that combination of compounds that target different levels of the above-mentioned signal transduction pathway might be more effective in eradication of breast cancer cells. In our in vitro research we used a commercially available drug, lapatinib, acting at the level of the receptor in combination with 1 of the plant-derived isothiocyanates: sulforaphane, erucin, or sulforaphene, as it has been shown previously that sulforaphane inhibits Akt-mTOR-S6K1 pathway in breast cancer cells. We used 2 HER2 overexpressing breast cancer cell lines, SKBR-3 and BT-474. Combinations of the drug and isothiocyanates considerably decreased their viability. This action was synergistic and was accompanied by a decrease in phosphorylation of HER2, Akt, and S6. Combined treatment induced apoptosis more efficiently than either agent alone; however the most effective was a combination of lapatinib with erucin. These findings might support the optimization of therapy based on lapatinib treatment.

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Section: Discussionmentioning

confidence: 99%

Sensitization of HER2 Positive Breast Cancer Cells to Lapatinib Using Plants-Derived Isothiocyanates

Kaczyńska

Swierczynska

Herman-Antosiewicz

2015

Nutrition and Cancer

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“…One important frontier in the study of cruciferous vegetables and health is to obtain better assessment of physiologically-relevant concentrations of individual vegetable constituents and their tissue metabolites. This difficult area is being developed in human trails, facilitated by such analytical techniques as LC-MS-MS and accelerator mass spectrometry [148][149][150].…”

Section: Future Perspectivesmentioning

confidence: 99%

Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis

Higdon

Delage

Williams

et al. 2007

Pharmacological Research

908

697

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Cruciferous vegetables are a rich source of glucosinolates and their hydrolysis products, including indoles and isothiocyanates, and high intake of cruciferous vegetables has been associated with lower risk of lung and colorectal cancer in some epidemiological studies. Glucosinolate hydrolysis products alter the metabolism or activity of sex hormones in ways that could inhibit the development of hormone-sensitive cancers, but evidence of an inverse association between cruciferous vegetable intake and breast or prostate cancer in humans is limited and inconsistent. Organizations such as the National Cancer Institute recommend the consumption of 5-9 servings of fruits and vegetables daily, but separate recommendations for cruciferous vegetables have not been established. Isothiocyanates and indoles derived from the hydrolysis of glucosinolates, such as sulforaphane and indole-3-carbinol (I3C), have been implicated in a variety of anticarcinogenic mechanisms, but deleterious effects also have been reported in some experimental protocols, including tumor promotion over prolonged periods of exposure. Epidemiological studies indicate that human exposure to isothiocyanates and indoles through cruciferous vegetable consumption may decrease cancer risk, but the protective effects may be influenced by individual genetic variation (polymorphisms) in the metabolism and elimination of isothiocyanates from the body. Cooking procedures also affect the bioavailability and intake of glucosinolates and their derivatives. Supplementation with I3C or the related dimer 3,3′-diindolylmethane (DIM) alters urinary estrogen metabolite profiles in women, but the effects of I3C and DIM on breast cancer risk are not known. Small preliminary trials in humans suggest that I3C supplementation may be beneficial in treating conditions related to human papilloma virus infection, such as cervical intraepithelial neoplasia and recurrent respiratory papillomatosis, but larger randomized controlled trials are needed.

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“…Experimental evidence, obtained in rats and humans, has shown the formation of ER also through in vivo reduction of the isothiocyanate SF, which structurally represents its oxidized analogue, characteristic of broccoli (Brassica oleracea L. ssp. italica) (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative Activity of the Dietary Isothiocyanate Erucin, a Bioactive Compound from Cruciferous Vegetables, on Human Prostate Cancer Cells

Melchini

Τράκα

Catania

et al. 2013

Nutrition and Cancer

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Association between Consumption of Cruciferous Vegetables and Condiments and Excretion in Urine of Isothiocyanate Mercapturic Acids

Cited by 75 publications

References 22 publications

Sensitization of HER2 Positive Breast Cancer Cells to Lapatinib Using Plants-Derived Isothiocyanates

Sensitization of HER2 Positive Breast Cancer Cells to Lapatinib Using Plants-Derived Isothiocyanates

Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis

Antiproliferative Activity of the Dietary Isothiocyanate Erucin, a Bioactive Compound from Cruciferous Vegetables, on Human Prostate Cancer Cells

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