Association between Antibody to the Chlamydial Heat-Shock Protein and Tubal Infertility

Toye, Baldwin; Laferrière, Craig A.; Claman, Paul; Jessamine, Peter; Peeling, Rosanna W.

doi:10.1093/infdis/168.5.1236

Cited by 170 publications

(105 citation statements)

References 28 publications

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“…A chlamydial protein, hsp60, has been suggested to be the mediator of detrimental effects of chlamydia on tubal function [116][117][118]. Chlamydial hsp60 can trigger both a humoral and cell-mediated immune response [119].…”

Section: Smokingmentioning

confidence: 99%

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

Ezzati

Djahanbakhch

Arian³

et al. 2014

J Assist Reprod Genet

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With the advent of assisted reproductive technology in the past three decades, the clinical importance of fallopian tubes has been relatively overlooked. However, successful spontaneous conception requires normal function of the tube to provide not only a conduit for the gametes to convene and embryo to reach the uterine cavity, but also a physiologically optimized environment for fertilization and early embryonic development. In this review, after a brief description of normal human tubal anatomy and histology, we will discuss tubal transport and its principal effectors, including ciliary motion, muscular contractility and tubal fluid. Furthermore, we will discuss the ciliary ultrastructure and regulation of ciliary beat frequency by ovarian steroids, follicular fluid, angiotensin system, autonomic nervous system and other factors such as adrenomedullin and prostaglandins. In the last section, we describe the adverse impact of various pathological conditions, such as endometriosis, infection and smoking on tubal function and ciliary motility.

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Section: Smokingmentioning

confidence: 99%

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

Ezzati

Djahanbakhch

Arian³

et al. 2014

J Assist Reprod Genet

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“…The introduction of commercial PCR in the routine clinical microbiology laboratory is an important advance in our ability to diagnose asymptomatic chlamydial infections in male partners of infertile couples (Toye et al, 1996;Gdoura et al, 2001). Nucleic acid amplification tests are considered to be a more suitable method for screening and diagnosing chlamydial infections, but one major problem with them is the presence of potential inhibitors in clinical specimens that can lead to false negative results.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Chlamydia trachomatis infection in asymptomatic male partners of infertile couples

Hamdad-Daoudi

Petit

2004

Journal of Medical Microbiology

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Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after prostatic massage (UPM) and semen specimens. Serum from each patient was also obtained and analysed for the presence of IgG and IgA chlamydial antibodies by in-house microimmunofluorescence (MIF) and pELISA. C. trachomatis was detected by PCR in 5 . 4 % of FVU samples, 2 . 7 % of semen specimens and in 0 . 9 % of UPM samples. Two treatments for processing the samples (storage at À70 8C and heating to 95 8C) were routinely used before initial testing to reduce the effects of inhibitors of PCR. Despite these precautions, the PCR method revealed the presence of inhibitors in 7 . 3 % of semen specimens and 3 . 6 % of FVU samples. C. trachomatis was detected by PCR COBAS AMPLICOR in seven of 111 patients (6 . 3 %) and by serology in five of 111 patients (4 . 5 %). The detection of C. trachomatis in FVU, UPM and semen specimens can serve as a marker for the presence of this organism in the genital tract, and can be used as a reliable way of detecting asymptomatic carriers of infection. INTRODUCTIONChlamydia trachomatis is one of the most common sexually transmitted pathogens of humans, with an estimated 92 million new cases occurring worldwide each year (WHO, 2001). The number of cases is probably underestimated, particularly for men, who are less likely to seek diagnosis than women. C. trachomatis often causes asymptomatic genital tract infections in both men and women, and the high number of unrecognized infected individuals provides a reservoir for spreading the infection to men and women via sexual contact.Chlamydial infection in the male urethra can be complicated by inflammation of the epididymis (Stamm et al., 1984) and the prostate gland (Dan et al., 1991), but the role that C. trachomatis infection plays in male infertility is controversial.C. trachomatis can attach to spermatozoa (Hanssen & Mardh, 1984) and can be present in cytoplasmic droplets of spermatozoa (Villegas et al., 1991). Previous studies have found a correlation between genital chlamydial infection and sperm quality (Custo et al., 1989), although other studies have reported contradictory findings (Eggert- Kruse et al., 1996;Gdoura et al., 2001). It is important to resolve this issue, since chlamydiae present in semen could be transmitted by adhering to spermatozoa, which may serve as vectors, spreading the pathogen to the uterus and Fallopian tubes. In fact, studies have reported finding spermatozoa with adhering chlamydial bodies in the peritoneal fluid of women with salpingitis at laparoscopy (Friberg et al., 1987).The presence of C. trachomatis in semen emphasizes the potential risk of this route of transmission, and underlines the need for sensitive direct detection methods in this group of patients (Dieterle et al., 1995).Sc...

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“…The involvement of the heat shock protein 60 (Hsp60) of C. trachomatis in the immunopathogenesis of trachoma [2], pelvic inflammatory disease [3], tubal infertility [4] and ectopic pregnancy [5] is well known. It is also known that persistent C. trachomatis infection in cell culture leads to increased expression of Hsp60 proteins in chlamydia particles [6].…”

mentioning

confidence: 99%

Host immune response toChlamydia pneumoniaeheat shock protein 60 is associated with asthma

et al. 2001

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Host immune response to Chlamydia pneumoniae heat shock protein 60 is associated with asthma. T. Huittinen, D. Hahn, T. Anttila, E. Wahlström, P. Saikku, M. Leinonen. #ERS Journals Ltd 2001. ABSTRACT: Chlamydia pneumoniae infection has been associated with asthma. It has also been suggested that heat shock protein 60 (Hsp60) belonging to a class of highly conserved proteins may play a role in the pathogenesis of chlamydial infections. The purpose was to study whether the host immune response to C. pneumoniae Hsp60 is associated with asthma and decreased pulmonary function.An enzyme immunoassay was used to measure immunoglobulin-(Ig)A and IgG antibodies against recombinant C. pneumoniae Hsp60 and human Hsp60 in a study group consisting of 24 cases of recently symptomatic asthma and 62 nonasthmatic controls.A strong (r~0.50) and significant (pv0.001) correlation was observed between C. pneumoniae and human Hsp60 IgA antibodies, but only C. pneumoniae Hsp60 IgA antibodies were significantly associated with asthma (p~0.02). Pulmonary function, as measured by forced expiratory volume in one second, also inversely correlated (r~-0.23, p~0.04) with the quantity of C. pneumoniae Hsp60 IgA antibodies, suggesting an association with the severity of pulmonary obstruction.By showing an association of Chlamydia pneumoniae heat shock protein 60 immunoglobulin A antibodies with asthma, the results support the hypothesis of an association between Chlamydia pneumoniae infection and asthma and support the need for further investigations on the role of heat shock protein 60 in the pathogenesis of asthma.

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Association between Antibody to the Chlamydial Heat-Shock Protein and Tubal Infertility

Cited by 170 publications

References 28 publications

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

Assessment of Chlamydia trachomatis infection in asymptomatic male partners of infertile couples

Host immune response toChlamydia pneumoniaeheat shock protein 60 is associated with asthma

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