“…Further production was carried out in one litre of mineral salt medium supplemented with 10 ml of overnight seed culture and 10% sucrose, maintained at 30°C for 4 days at 180 rpm. Subsequent centrifugation of culture at 5000 rpm for 15 min precipitated β-glucan in ice cold ethanol to produce pellets from where the crude glucan was obtained by lyophilization 5 .…”
“…Further production was carried out in one litre of mineral salt medium supplemented with 10 ml of overnight seed culture and 10% sucrose, maintained at 30°C for 4 days at 180 rpm. Subsequent centrifugation of culture at 5000 rpm for 15 min precipitated β-glucan in ice cold ethanol to produce pellets from where the crude glucan was obtained by lyophilization 5 .…”
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