Association Analysis of Polymorphisms in the 5′ Flanking Region of the HSP70 Gene with Blood Biochemical Parameters of Lactating Holstein Cows under Heat and Cold Stress

Abbas, Zaheer; Hu, Lirong; Fang, Hua; Sammad, Abdul; Lee, Kang; Brito, Luiz F.; Chen, Nansheng; Wang, Yachun

doi:10.3390/ani10112016

Cited by 13 publications

(16 citation statements)

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“…Concentration of eHsp70 in saliva in cows ranged from 0.524 to 12.174 ng/mL [30]. Hsp70 is significantly regulated by high and low ambient temperatures and is a very sensitive marker of heat stress in cows [34]. The cows in this experiment calved in March and April, which are markedly thermoneutral months in our geographic region [35], so the external ambient temperatures did not affect the value of eHsp70.…”

Section: Discussionmentioning

confidence: 74%

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

et al. 2021

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Metabolic stress in early lactation cows is characterized by lipolysis, ketogenesis, insulin resistance and inflammation because of negative energy balance and increased use of lipids for energy needs. In this study the relationship between lipid metabolite, lipid-based insulin resistance, and hepatocyte functionality indexes and tumor necrosis factor alpha (TNF-α) with extracellular heat shock protein 70 (eHsp70) was investigated. The experiment included 50 cows and all parameters were measured in blood serum. In cows with a more pronounced negative energy balance, the following was determined: a higher concentration of eHsp70, TNF-α, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHB), NEFA to insulin and NEFA to cholesterol ratio and lower concentration of cholesterol, very low-density lipoproteins (VLDL), low density lipoproteins (LDL) and liver functionality index (LFI). The eHsp70 correlated negatively with the values of cholesterol, VLDL, LDL, and triglycerides, while correlated positively with the level of NEFA and BHB. A higher concentration of eHsp70 suggests the development of fatty liver (due to a higher NEFA to cholesterol ratio and lower LFI) and insulin resistance (due to a lower revised quantitative insulin sensitivity check index RQUICKI-BHB and higher NEFA to insulin ratio). The eHsp70 correlated positively with TNF-α. Both TNF-α and eHsp70 correlated similarly to lipid metabolites. In cows with high eHsp70 and TNF-α values we found higher concentrations of NEFA, BHB, NEFA to insulin and NEFA to cholesterol ratio and a lower concentration of triglycerides and VLDL cholesterol compared to cows that had only high TNF-α values. Based on the positive correlation between eHsp70 and TNF-α, their similar relations, and the additional effect of eHsp70 (high TNF-α + eHsp70 values) on lipid metabolites we conclude that eHsp70 has pro-inflammatory effects implicating lipolysis, fatty liver, and fat tissue insulin resistance.

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Section: Discussionmentioning

confidence: 74%

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

et al. 2021

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“…Besides, our previous studies have found that Sanhe cattle after exposure to extreme cold stress, only HSPA1A expression was upregulated among 27 genes encoding HSP-binding proteins, heat shock transcription factors, and heat shock proteins ( Xu et al, 2017 ). The expression of HSPA1A was related to the levels of blood hormones ( Hu et al, 2019 ; Abbas et al, 2020a ). Consistent with our present study, 25°C was already adopted as the mild cold shock treatment temperature to study cold stress response in WI26 cells ( Neutelings et al, 2013 ), whereas 42°C was adopted to simulate heat stress conditions for Korean native male beef calves ( Kim et al, 2020 ), riverine buffaloes (Bubalus bubalis), exotic cattle (Bos taurus), and native cattle (Bos indicus) of India ( Kishore et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of key Genes and Pathways Associated With Thermal Stress in Peripheral Blood Mononuclear Cells of Holstein Dairy Cattle

et al. 2021

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The objectives of the present study were to identify key genes and biological pathways associated with thermal stress in Chinese Holstein dairy cattle. Hence, we constructed a cell-model, applied various molecular biology experimental techniques and bioinformatics analysis. A total of 55 candidate genes were screened from published literature and the IPA database to examine its regulation under cold (25°C) or heat (42°C) stress in PBMCs. We identified 29 (3 up-regulated and 26 down-regulated) and 41 (15 up-regulated and 26 down-regulated) significantly differentially expressed genes (DEGs) (fold change ≥ 1.2-fold and P < 0.05) after cold and heat stress treatments, respectively. Furthermore, bioinformatics analyses confirmed that major biological processes and pathways associated with thermal stress include protein folding and refolding, protein phosphorylation, transcription factor binding, immune effector process, negative regulation of cell proliferation, autophagy, apoptosis, protein processing in endoplasmic reticulum, estrogen signaling pathway, pathways related to cancer, PI3K- Akt signaling pathway, and MAPK signaling pathway. Based on validation at the cellular and individual levels, the mRNA expression of the HIF1A gene showed upregulation during cold stress and the EIF2A, HSPA1A, HSP90AA1, and HSF1 genes showed downregulation after heat exposure. The RT-qPCR and western blot results revealed that the HIF1A after cold stress and the EIF2A, HSPA1A, HSP90AA1, and HSF1 after heat stress had consistent trend changes at the cellular transcription and translation levels, suggesting as key genes associated with thermal stress response in Holstein dairy cattle. The cellular model established in this study with PBMCs provides a suitable platform to improve our understanding of thermal stress in dairy cattle. Moreover, this study provides an opportunity to develop simultaneously both high-yielding and thermotolerant Chinese Holstein cattle through marker-assisted selection.

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“…Inner-Mongolia Sanhe is a dual-purpose cattle breed in China that has been an important genetic resource for studying the effects of cold stress response on blood biochemical parameters [ 31 ], gene expression profile in the peripheral blood [ 9 ], characterization of genetic variation related to cold tolerance [ 14 ], and signatures of selection related to thermal tolerance [ 32 ]. However, serum metabolome and metabolic regulation in response to severe cold exposure had not been studied in this population.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, "phenylalanine metabolism", "pyruvate metabolism", "propanoate metabolism", and "glycolysis/gluconeogenesis" were identified and are mainly related to five metabolites (propionate, acetate, phenylalanine, valine, and leucine). We then performed the integration analysis based on the datasets of 19 differential metabolites and 193 candidate genes from our previous study [9], with detailed results provided in Supplementary Table S4. Eight metabolic pathways were identified (Figure 7b), in which five genes, including three downregulated (selenophosphate synthetase 1, SEPHS1; enoyl coenzyme A hydratase domain containing 1, ECHDC1; dihydrolipoamide dehydrogenase, DLD) and two upregulated (arginyl-tRNA synthetase, RARS; tryptophanyl-tRNA synthetase, WARS) genes (Figure 7c) were found to play functional roles in the metabolic pathways of ten related metabolites (propionate, acetate, phenylalanine, valine, leucine, creatine, betaine, 3-hydroxyisobutyrate, methionine, and alanine).…”

Section: Key Metabolic Pathways Involved In Severe Cold Stressmentioning

confidence: 99%

“…Furthermore, according to a recent report from the Intergovernmental Panel on Climate Change (IPCC), the effects of climate change, including extreme weather events, are expected to further increase in the near future [ 8 ]. A better understanding of the biological mechanisms underlying thermal stress in livestock is paramount for developing mitigation strategies to minimize the negative effects of both heat and cold stress [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle

Brito

Abbas

et al. 2021

Animals

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Inner-Mongolia Sanhe cattle are well-adapted to low-temperature conditions, but the metabolic mechanisms underlying their climatic resilience are still unknown. Based on the 1H Nuclear Magnetic Resonance platform, 41 metabolites were identified and quantified in the serum of 10 heifers under thermal neutrality (5 °C), and subsequent exposure to hyper-cold temperature (−32 °C) for 3 h. Subsequently, 28 metabolites were pre-filtrated, and they provided better performance in multivariate analysis than that of using 41 metabolites. This indicated the need for pre-filtering of the metabolome data in a paired experimental design. In response to the cold exposure challenge, 19 metabolites associated with cold stress response were identified, mainly enriched in “aminoacyl-tRNA biosynthesis” and “valine, leucine, and isoleucine degradation”. A further integration of metabolome and gene expression highlighted the functional roles of the DLD (dihydrolipoamide dehydrogenase), WARS (tryptophanyl-tRNA synthetase), and RARS (arginyl-tRNA synthetase) genes in metabolic pathways of valine and leucine. Furthermore, the essential regulations of SLC30A6 (solute carrier family 30 (zinc transporter), member 6) in metabolic transportation for propionate, acetate, valine, and leucine under severe cold exposure were observed. Our findings presented a comprehensive characterization of the serum metabolome of Inner-Mongolia Sanhe cattle, and contributed to a better understanding of the crucial roles of regulations in metabolites and metabolic pathways during cold stress events in cattle.

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Association Analysis of Polymorphisms in the 5′ Flanking Region of the HSP70 Gene with Blood Biochemical Parameters of Lactating Holstein Cows under Heat and Cold Stress

Cited by 13 publications

References 76 publications

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

Identification of key Genes and Pathways Associated With Thermal Stress in Peripheral Blood Mononuclear Cells of Holstein Dairy Cattle

Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle

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