Association analysis of DNA methylation and the tissue/developmental expression of the FUT3 gene in Meishan pigs

Li, Yang; Liu, Huan; Cheng, Jinhua; Wu, Zhengchang; Bao, Wenbin; Wu, Shenglong

doi:10.1016/j.gene.2022.147016

Cited by 4 publications

(1 citation statement)

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“…When CGI undergo methylation modification, the methylation level of promoter region changes and generally accompanied by the inhibition of gene transcription, resulting in gene silencing ( 26 , 27 ). The distribution of upstream and downstream methylation levels of genes indicated high methylation levels in this genomic region, which is common in mammalian, especially in aquatic animals, and negatively regulated the level of gene expression ( 28 , 29 ). These results indicated that the spontaneous DNA methylation pattern in turbot was similar to other species, indicating that the DNA methylation patterns among vertebrates are relatively conserved to a certain extent.…”

Section: Discussionmentioning

confidence: 99%

Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation

Lin²,

Liu³

et al. 2023

Front. Immunol.

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IntroductionDNA methylation was one of the most important modification in epigenetics and played an important role in immune response. Since the introduction of Scophthalmus maximus, the scale of breeding has continued to expand, during which diseases caused by various bacteria, viruses and parasites have become increasingly serious. Therefore, the inactivated vaccines have been widely researched and used in the field of aquatic products with its unique advantages. However, the immune mechanism that occurred in turbot after immunization with inactivated vaccine of Aeromonas salmonicida was not clear.MethodsIn this study, differentially methylated regions (DMRs) were screened by Whole Genome Bisulfite Sequencing (WGBS) and significantly differentially expressed genes (DEGs) were screened by Transcriptome sequencing. Double luciferase report assay and DNA pull-down assay were further verified the DNA methylation state of the gene promoter region affected genes transcriptional activity after immunization with inactivated vaccine of Aeromonas salmonicida.ResultsA total of 8149 differentially methylated regions (DMRs) were screened, in which there were many immune-related genes with altered DNA methylation status. Meanwhile, 386 significantly differentially expressed genes (DEGs) were identified, many of which were significantly enriched in Toll-like receptor signaling pathway, NOD-like receptor signaling pathway and C-type lectin receptor signaling pathway. Combined analysis of WGBS results and RNA-seq results, a total of 9 DMRs of negatively regulated genes are located in the promoter region, including 2 hypermethylated genes with lower expression and 7 hypomethylated genes with higher expression. Then, two immune-related genes C5a anaphylatoxin chemotactic receptor 1-like (C5ar1-Like) and Eosinophil peroxidase-like (EPX-Like), were screened to explore the regulation mechanism of DNA methylation modification on their expression level. Moreover, the DNA methylation state of the gene promoter region affected genes transcriptional activity by inhibiting the binding of transcription factors, which lead to changes in the expression level of the gene.DiscussionWe jointly analyzed WGBS and RNA-seq results and revealed the immune mechanism that occurred in turbot after immunized with inactivated vaccine of A. salmonicida from the perspective of DNA methylation.

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