Association Analyses with Carcass Traits in the Porcine KIAA1717 and HUMMLC2B Genes

Abstract

By screening a subtracted cDNA library constructed with mRNA obtained from the longissimus dorsi muscles of F1 hybrids Landrace×Yorkshire and their Yorkshire female parents, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. In the present work we investigated two SNPs, one (C1354T) at the 3' untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, in a resource population derived from crossing Chinese Meishan and Large White pig. The selected pigs were genotyped by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with thorax-waist backfat thickness (p<0.05), buttock backfat thickness (p<0.05), average backfat thickness (p<0.05), loin eye height (p<0.05), loin eye area (p<0.05), carcass length to 1 st spondyle (p<0.01) and carcass length to 1 st rib (p<0.01). HUMMLC2B A345G were significantly associated with loin eye width (p<0.05), loin eye area (p<0.05). Further studies are needed to confirm these preliminary results.

Associations of MYF5 gene polymorphisms with meat quality traits in different domestic pig (Sus scrofa) populations

“…In some cases both genes and the underlying causal mutation have been identified by the candidate gene approach (Harlizius and van der Lende, 2001;Li et al, 2002;Xu et al, 2005). Animal breeders have started applying markerassisted selection to improve the quality and performance of livestock (van der Steen et al, 2005) and genetic polymorphisms (marker loci) significantly associated with important traits have become very useful tools.…”

3’-UTR polymorphisms of SETD7 gene associate with economic traits in an F2 population between Landrace and Jeju (Korea) Black pigs

“…However, the marbling scores, which reflect the levels of Several quantitative trait loci (QTL) for meat quality traits including backfat and total body fat (Rohrer, 2000;Edwards et al, 2008) and physiological traits in blood such as alkaline phosphatase activity (Yoo et al, 2012), CD4positive leukocyte number (Wattrang et al, 2005), and bilirubin levels (Reiner et al, 2008) (www.animalgenome.org/ cgi-bin/QTLdb) are mapped at the region on Sus scrofa chromosome 8 (SSC8) where SETD7 is located. Reports of an F 2 population of an intercross between Large White and Chinese Meishan pigs described a significant difference in the levels of backfat thickness (Xu et al, 2007), marbling score, and intramuscular fat percentage (Xu et al, 2005), according to the SETD7 genotypes. Lee et al (2011) also reported a significant difference in various traits, including backfat thickness, in a comparison of each genotype.…”

“…A set of primers designed by Xu et al (2005) was used for SETD7 gene amplification and genotyping. PCR was performed using 25 ul of reaction mixture including 100 ng of DNA, 1.0 nmole of each primer, and 2.5 U of i-Taq DNA polymerase (Intron Biotechnology, Korea).…”

“…The PCR products were separated on agarose gels and visualized by UV-illumination. SETD7 g.1354C>T polymorphism was genotyped using MspI PCR-RFLP and subsequent agarose gel electrophoresis according to Xu et al (2005). After genotyping, two primary PCR products from each homozygote for g.1354C/C and g.1354T/T were directly sequenced with a MegaBase1000 automated sequencer (Amersham-Pharmacia, USA), respectively.…”

“…In pig, SETD7 cDNA was found to be one of differentially displayed genes from a porcine M. logissimus dorsi subtracted cDNA library, which was highly expressed in Large White dams. A single nucleotide polymorphism for g.1354C>T was found at the 3'-UTR of the SETD7 gene (Xu et al, 2005). Furthermore, significant associations have been also detected among the genotype variation and some economic traits, including the levels of intramuscular fat deposition in an intercross population of Large White and Chinese Meishan pigs (Xu et al, 2005(Xu et al, , 2007 and Berkshire pigs (Lee et al, 2011).…”