Association among lipopolysaccharide, the transforming growth factor–beta superfamily, follicular growth, and transcription factors in spontaneous bovine ovarian cysts

Çolakoğlu, Hatice Esra; Küplülü, Şükrü; Polat, İbrahim; Pekcan, Mert; Özenç, Erhan; Baklacı, C.; Seyrek-İntaş, K.; Gümen, Ahmet; Vural, Mehmet Rıfat

doi:10.1016/j.domaniend.2019.106398

Cited by 4 publications

(4 citation statements)

References 56 publications

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“…When bovine follicular cysts, estrus is abnormal, estrus cycle becomes short, and estrus period is prolonged, or there is sustained and strong estrus phenomenon defined as nymphomania ( 46 ). Follicular cysts are single or multiple, in one or both ovaries, with diameter at least 2.5 cm ( 47 ). The ovarian wall is thin and dense, filled with cystic fluid, and slightly fluctuating.…”

Section: Discussionmentioning

confidence: 99%

Analyzing the interactions of mRNAs, miRNAs and lncRNAs to predict ceRNA networks in bovine cystic follicular granulosa cells

et al. 2022

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Cross-talk between competitive endogenous RNAs (ceRNAs) may play a critical role in revealing potential mechanism of bovine follicular cysts. Ovarian cyst has always been an intractable scientific problem and has led to considerable economic losses to bovine breeding industry. However, its pathogenesis and molecular mechanisms are still not well understood. Here, this study aimed to investigate the role of non-coding RNAs (ncRNAs) and the ceRNA networks in bovine follicular cyst. Whole transcriptome sequencing of bovine follicular granulosa cells (GCs) was conducted to obtain the expression profiles of mRNAs, lncRNAs and miRNAs. The results for the identified expressions of 8,003 mRNAs, 579 lncRNAs and 205 miRNAs were often altered between cystic and normal follicular GCs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed on these differentially expressed mRNAs. Furthermore, the ceRNA network combining mRNAs, miRNAs, and lncRNAs using several bioinformatics methods based on co-expression analysis between the differentially expressed RNAs was conducted. Finally, the lncRNA NONBTAT027373.1-miR-664b-HSD17B7 pathway was verified by dual-luciferase reporting assay and RNA binding protein immunoprecipitation (RIP) assay. LncRNA NONBTAT027373.1 sponged miR-664b in GCs and prevented miR-664b from binding to the HSD17B7 3′-UTR. These results indicated that genes and lncRNAs related to steroid hormone synthesis and energy metabolism could play important roles in the formation of bovine cystic follicles through the ceRNA mechanism and represent candidate targets for further research. This can be used as a practical guideline for promoting healthy and highly efficient development in the bovine industry.

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Section: Discussionmentioning

confidence: 99%

Analyzing the interactions of mRNAs, miRNAs and lncRNAs to predict ceRNA networks in bovine cystic follicular granulosa cells

et al. 2022

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“…To assess the effects of Res on GC steroidogenesis, cells were cultured in the presence of Res (50 μmol/L) for 12 h in either basal media (5 mM glucose) or high-glucose media (30 mM glucose). Media was then harvested and stored at −20°C for subsequent analysis, while GCs were stored at −80°C prior to subsequent qPCR and caspase-3 activity assays (14). Experiments were conducted in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Resveratrol protects against high glucose-induced steroidogenesis and apoptosis in murine granulosa cells

Ning Zhang,

Yinghua Shan,

Huayu Lian

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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Resveratrol (Res) is a polyphenolic compound that exhibits a diverse array of biological effects. Herein, we detected the ability of Res on murine granulosa cells (GCs) against impaired steroidogenesis and apoptotic death in response to high glucose levels. Ovarian GCs were harvested from C57BL/6 mice and cultured in steroidogenic media supplemented with follicle-stimulating hormone (FSH, 30 ng/mL), Res (50 μmol/L), and low or high glucose concentrations (5 mM or 30 mM). After culture for 24 h, cell supernatants were harvested and the levels of progesterone and estradiol therein were measured. Also, caspase-3 activity and the expression of genes associated with apoptosis and steroidogenesis were assessed. High-glucose treatment suppressed steroidogenesis in this assay system, resulting in the impaired expression of steroidogenesis-related genes including Cyp11a1, Cyp19a1, 3βHSD, and StAR and a concomitant decrease in progesterone and estradiol production. Cells exposed to high glucose also exhibited apoptotic phenotypes characterized by Bax upregulation, Bcl-2 downregulation, and increased caspase-3 activity levels. However, Res treatment was sufficient to reverse this high glucose level-induced apoptotic and steroidogenic phenotypes with improving progesterone and estradiol production, and these maybe related the effects of Res on Cyp11a1, Cyp19a1, 3βHSD, and StAR expressions. These data suggested that Res is well suited to overcoming the negative effects of hyperglycemia of GC functionality.

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“…Regarding the effects of COD on oviductal function, one of the most interesting clinical findings is that COD cows are more likely to exhibit endometritis than the control cows. The co-existence of COD and endometritis has already been mentioned (Çolakoğlu et al, 2020;Gobikrushanth et al, 2016;Purba et al, 2021). However, to date, it remains to be elucidated whether (1) inflammation supports the development of COD by promoting the failure in the ovulation of the preovulatory follicle and preventing subsequent atresia, or whether…”

Section: Cod Alters the Synthesis Of Acidic Mucopolysaccharides In Th...mentioning

confidence: 99%

Cystic ovary disease (COD) alters structure and function of the bovine oviduct

Scully,

Reese,

Kölle

2024

Molecular Reproduction Devel

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Cystic ovary disease (COD) is a common cause of subfertility in dairy cattle. Therefore, the aim of this study was to provide novel concepts for cyst classification and to investigate the effects of COD on tubal microarchitecture, oviductal metabolic function, and the formation of the sperm reservoir. Bovine Fallopian tubes affected by follicular cysts, follicular cysts with luteinization and luteal cysts were investigated by a variety of microscopic and histological techniques and compared to control cows in metestrus and diestrus. We defined three types of cysts involved in COD, each of which had a characteristic wall thickness, inner wall appearance and cellular pattern within the cyst aspirate. Regarding the Fallopian tube, each cyst type was associated with a characteristic morphology, specifically the microarchitecture of the folds in ampulla, epithelial cell ratios, and ciliated/secretory cell size and form. Furthermore, each cyst type showed different patterns of tubal glycoprotein and acidic mucopolysaccharide synthesis, which was highly variable as compared to the controls. Our studies are the first to characterize the effects of COD on the Fallopian tube, which promotes the establishment of novel, cyst‐specific therapeutic concepts in cattle and helps gain a holistic view of the causes of subfertility in cows with COD.

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Association among lipopolysaccharide, the transforming growth factor–beta superfamily, follicular growth, and transcription factors in spontaneous bovine ovarian cysts

Cited by 4 publications

References 56 publications

Analyzing the interactions of mRNAs, miRNAs and lncRNAs to predict ceRNA networks in bovine cystic follicular granulosa cells

Analyzing the interactions of mRNAs, miRNAs and lncRNAs to predict ceRNA networks in bovine cystic follicular granulosa cells

Resveratrol protects against high glucose-induced steroidogenesis and apoptosis in murine granulosa cells

Cystic ovary disease (COD) alters structure and function of the bovine oviduct

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