Associated Bacterial Coinfections in COVID-19-Positive Patients

Boia, Eugen Radu; Huț, Alexandru Romulus; Roi, Alexandra; Luca, Ruxandra Elena; Munteanu, Ioana Roxana; Roi, Ciprian Ioan; Riviș, Mircea; Boia, Simina; Duse, Adina Octavia; Vulcănescu, Dan Dumitru; Horhat, Florin George

doi:10.3390/medicina59101858

Cited by 3 publications

(1 citation statement)

References 53 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several authors assume that virtually all cases of aggressive periodontitis involve an active herpesvirus infection, which has the potential to impair periodontal host defenses and induce an overgrowth of pathogenic bacteria [4][5][6][7][8][9][10][11][12]. Viral DNA has been detected in gingival tissue, gingival crevicular fluid, and subgingival plaque from periodontally affected sites [13].…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost Protocol Using the Adjunctive Action of Povidone–Iodine Irrigations and Sodium Hypochlorite Rinsing Solution in Step 2 of Periodontal Therapy for Patients with Stage III–IV Periodontitis: A Single-Blind, Randomized Controlled Trial

Kardaras,

Christodorescu,

Boariu

et al. 2024

Dentistry Journal

Self Cite

View full text Add to dashboard Cite

In severe stages of periodontitis, conventional periodontal therapy and maintenance care are usually insufficient due to the viral and bacterial etiology; thus, a mechanical approach alone may not be sufficient to eliminate a substantial portion of subgingival pathogens, especially in deep periodontal sites. Background and Objectives: This single-blind, randomized clinical trial aimed to compare the clinical and microbiological efficacy of a low-cost protocol using povidone–iodine and sodium hypochlorite formulations as adjuncts to non-surgical therapy for patients with stage IV periodontitis when compared with chlorhexidine, the most commonly employed substance to date for antimicrobial regimens in periodontal therapy. Materials and Methods: Forty-five patients were randomly divided into two groups: control (subgingival instrumentation, chlorhexidine-assisted) and test (antiviral medication, subgingival instrumentation with povidone–iodine, sodium hypochlorite rinsing solution, and antibiotics). Clinical measurements and microbiological analyses were performed at baseline and after three months. Results: After three months, notable differences were found in the bacterial detection scores for Porphyromonas gingivalis (a significant reduction in detection frequency was observed in the test compared to the control (p = 0.021)), and there were significant reductions in detection in the test group for Tannerella forsythia and Treponema denticola, showing undetectable levels (p < 0.0001 for both). In the test group, the pocket probing depth median value was reduced significantly (p = 0.0005); similarly, bleeding on probing showed a marked decrease (p < 0.0001). However, changes in clinical attachment loss and full-mouth plaque score were not statistically significant. Conclusions: Using the proposed protocol, substantial improvements in clinical and microbiological parameters were obtained when compared with the current antimicrobial recommendations.

show abstract

Section: Introductionmentioning

confidence: 99%

A Low-Cost Protocol Using the Adjunctive Action of Povidone–Iodine Irrigations and Sodium Hypochlorite Rinsing Solution in Step 2 of Periodontal Therapy for Patients with Stage III–IV Periodontitis: A Single-Blind, Randomized Controlled Trial

Kardaras,

Christodorescu,

Boariu

et al. 2024

Dentistry Journal

Self Cite

View full text Add to dashboard Cite

show abstract

Microbiological profile of patients with orthopedic implant-associated infection in the post-COVID period

Lyubimova,

Pchelova,

Nikolaev

et al. 2024

Acta biomedica scientifica

View full text Add to dashboard Cite

Background. The etiological structure of implant-associated infection and antibiotic resistance of pathogens are important when choosing empirical antibiotic therapy. COVID-19 pandemic and increased consumption of antibiotics by the population could provoke an increase in antibiotic resistance.The aim of the work. To compare the spectrum of leading pathogens of implantassociated infection in the pre- and post-Covid period and to assess antibiotic resistance.Materials and methods. A continuous retrospective study of biomaterial samples from traumatology and orthopedic patients with implant-associated infection was carried out for 2018–2019 and 2021–2022. The sample consisted of 548 microorganism strains (n = 237 and n = 317, respectively) in 442 cases of infectious complications. The antibiotic resistance of all isolated microorganisms, including those from microbial associations, was assessed.Results. The leading pathogen of monomicrobial implant-associated infection in both study periods was Staphylococcus epidermidis (33–37 %). In 2021–2022, the proportion of microbial associations increased (from 12.5 to 17.5 %; p = 0.147) with the appearance of fungi in the microbial landscape. In the post-Covid period, the increase in Staphylococcus aureus resistance to tetracycline and doxycycline was revealed; the isolation of methicillin-resistant strains among Staphylococcus aureus decreased from 4 cases (out of 187) to 3 (out of 232); 100 % sensitivity to rifampicin and co-trimoxazole was maintained. An increase in Staphylococcus epidermidis resistance to all tested antibiotics was detected (statistically significant increase in resistance to fluoroquinolones; p = 0.002–0.003) with the isolation of methicillin-resistant strains in 80.5% and 80.9% of cases, respectively. All staphylococcal isolates were susceptible to vancomycin and linezolid. Enterobacteriaceae representatives showed a decrease in resistance to carbapenems and an increase in resistance to co-trimoxazole; in Pseudomonas aeruginosa and Acinetobacter baumannii, there is an increase in resistance to carbapenems and fluoroquinolones. All gram-negative microorganisms were sensitive to colistin.Conclusion. The high frequency of isolation of methicillin-resistant staphylococci determines the choice of vancomycin for empirical therapy. Increasing resistance of staphylococci to fluoroquinolones may limit their use. Increasing resistance of gram-negative bacteria and a narrow spectrum of antibiotics acting on carbapenemase producers may reduce the effectiveness of therapy.

show abstract

Histologic Evaluation of Early Papilla Healing after Augmentation with Injectable Hyaluronic Acid—A Proof of Concept

Vela,

Boariu,

Iorio-Siciliano

et al. 2024

JCM

View full text Add to dashboard Cite

Objectives: This human histological study’s purpose was to histologically evaluate papillae’s healing after hyaluronic acid (HA) gel augmentation at three healing time points after one injection with hyaDENT BG®. Methods: Fifteen papillae from two patients with stage III, grade B periodontitis have been selected for this study. Every week for three weeks, five papillae were injected once with HA gel, and during the fourth week, the papillae were surgically removed as part of step 3 of the periodontal treatment. The histological analysis was performed on fifteen papillae, with five papillae corresponding to every timepoint of healing (weeks 1, 2, and 3). The primary outcome was considered to be the newly formed collagen fibers. The presence of residual HA, the integrity of epithelium or the presence of erosions/ulcerations, the presence and characteristics of inflammatory infiltrate, the presence of granulomatous reactions, and interstitial edema were considered to be secondary outcomes. Results: From the first to the third week, newly formed connective tissue begins to appear, while the observed HA pools (vesicles) content decreases. The density of inflammatory infiltrate was higher in the first week after injection, decreasing considerably by week 3; however, it was still visible throughout the healing time points. A granulomatous reaction was present in only three samples, while no signs of ulceration or necrosis could be observed; however, epithelial erosions could be observed on some samples after the first week. Conclusions: Papila augmentation with hyaluronic acid promotes new collagen formation from the second week of healing despite some foreign body granulomatous reactions.

show abstract

Associated Bacterial Coinfections in COVID-19-Positive Patients

Cited by 3 publications

References 53 publications

A Low-Cost Protocol Using the Adjunctive Action of Povidone–Iodine Irrigations and Sodium Hypochlorite Rinsing Solution in Step 2 of Periodontal Therapy for Patients with Stage III–IV Periodontitis: A Single-Blind, Randomized Controlled Trial

A Low-Cost Protocol Using the Adjunctive Action of Povidone–Iodine Irrigations and Sodium Hypochlorite Rinsing Solution in Step 2 of Periodontal Therapy for Patients with Stage III–IV Periodontitis: A Single-Blind, Randomized Controlled Trial

Microbiological profile of patients with orthopedic implant-associated infection in the post-COVID period

Histologic Evaluation of Early Papilla Healing after Augmentation with Injectable Hyaluronic Acid—A Proof of Concept

Contact Info

Product

Resources

About