Associated Autoimmunity in Addison's Disease

Zelissen, Pierre; Bast, E. J. E. G.; Croughs, R. J. M.

doi:10.1006/jaut.1995.0009

Cited by 175 publications

(129 citation statements)

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“…The diagnostic sensitivity of the PLPA technique for autoimmune AD was not statistically different from that of the IIF assay. The frequency of ACA observed in "idiopathic" AD patients was similar to that found in previous studies in other countries (2)(3)(4)(5)(6).…”

Section: Discussionsupporting

confidence: 89%

“…In Western countries, autoimmune adrenalitis accounts for approximately 65 to 90% of the cases of primary adrenocortical insufficiency (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…The presence of circulating adrenal cortex autoantibodies (ACA) is the major marker of autoimmune AD, being typically found in 60 to 80% of patients with idiopathic AD (2)(3)(4)(5)(6). Conventionally, ACA are detected by indirect immunofluorescence (IIF) on fro-males; median age, 54 years; range, 34-85 years; average disease duration of 5 years, range 0.1-38 years), followed since 1988 in the Division of Endocrinology at the Federal University of São Paulo.…”

Section: Introductionmentioning

confidence: 99%

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Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique

Silva

Faiçal

Laureti

et al. 1998

Braz J Med Biol Res

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Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera.

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Section: Discussionsupporting

confidence: 89%

“…In Western countries, autoimmune adrenalitis accounts for approximately 65 to 90% of the cases of primary adrenocortical insufficiency (2,3).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique

Silva

Faiçal

Laureti

et al. 1998

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Most frequently AAD is associated with Hashimoto's thyroiditis or Graves's disease, one or the other being present in over 50% of patients with autoimmune adrenal insufficiency (Betterle et al, 2002;Nerup, 1974;Kong et al, 1994;Irvine et al, 1967Irvine et al, , 1979McHardy-Young et al, 1974;De Rosa et al, 1987;Papadopoulos et al, 1990;Kasperlik-Zaluska et al, 1991;Zelissen et al, 1995;Söderbergh et al, 1996;Neufeld et al, 1981). Atrophic gastritis is present in 20-25% of AAD patients, while type 1 diabetes mellitus (T1DM) has been reported in 1-20% of addisonian patients (Betterle et al, 2002;Nerup, 1974;Kong et al, 1994;Irvine et al, 1967Irvine et al, , 1979McHardy-Young et al, 1974;De Rosa et al, 1987;Papadopoulos et al, 1990;Kasperlik-Zaluska et al, 1991;Zelissen et al, 1995;Söderbergh et al, 1996;Neufeld et al, 1981). Hypergonadotropic hypogonadism is present in 4.5-19% of AAD women.…”

Section: Introductionmentioning

confidence: 99%

“…Hypergonadotropic hypogonadism is present in 4.5-19% of AAD women. Other autoimmune diseases found associated with AAD at lower frequency include: vitiligo, alopecia, celiac disease, pernicious anemia, multiple sclerosis, inflammatory bowel disease, Sjögren's syndrome, chronic hepatitis and lymphocytic hypophysitis (Betterle et al, 2002;Nerup, 1974;Kong et al, 1994;Irvine et al, 1967Irvine et al, , 1979McHardy-Young et al, 1974;De Rosa et al, 1987;Papadopoulos et al, 1990;Kasperlik-Zaluska et al, 1991;Zelissen et al, 1995;Söderbergh et al, 1996;Neufeld et al, 1981).…”

Section: Introductionmentioning

confidence: 99%