Assisted reproductive technologies in the reproductive management of small ruminants

Amiridis, G.S.; Cseh, Sándor

doi:10.1016/j.anireprosci.2012.01.009

Cited by 95 publications

(91 citation statements)

References 132 publications

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“…There are several techniques of oocyte collection, namely, follicular aspiration or ovary slicing in abattoirderived ovaries [110,111] and ovum pick-up systems applied in living animals. Ovary slicing technique provides cumulus-oocyte complexes (COCs) of better quality and the number of harvested oocytes collected is higher compared to follicle aspiration [110,112]. Sheep ovum pick-up systems are applied in living animals using minimal invasive procedures such as laparoscopic ovum pick-up (LOPU) technique guided by laparoscopy [113][114][115].…”

Section: Collection Of Oocytesmentioning

confidence: 99%

“…This quality depends on the competence of the oocyte to complete meiosis, which is affected by the follicle size [35]. It is also clearly established that prepubertal oocytes do not have the developmental potential of those collected from adult ewes [118], although, in the future, the results obtained with juvenile oocyte collection could have potential interest for decreasing half the generation interval in genetic programs [112].…”

Section: Collection Of Oocytesmentioning

confidence: 99%

“…IVM is usually performed at 38-39°C in a humidified atmosphere in air and 5% CO 2 [8,18,50,112]. Tissue Culture Medium (TCM199) supplemented with bicarbonate, pyruvate, LH, FSH, and estradiol (E 2 ) and with 10% fetal calf serum [10,101] or 10% estrous sheep serum [50,122] is the widest used medium for IVM.…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

“…IVC, on the contrary, is achieved in a controlled atmosphere at 38.5-39°C with 5% O 2 , 5% CO 2 , and 90% N 2 . This medium is usually composed of synthetic oviductal fluid supplemented with amino acids, bovine serum albumin (BSA), and/or serum [112,122], although the use of some media with oviduct cells or fetal fibroblasts was also reported [138]. In this stage, it is important to keep an oxygen low concentration to prevent oxidation [139] and the deleterious effects of reactive oxygen species (ROS).…”

Section: In Vitro Culture (Ivc)mentioning

confidence: 99%

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Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservation are unattractive for commercial purposes. It is expected that a successful cryopreservation of sheep embryos can push forward all other reproductive biotechnologies in this species, such as multiple ovulation and embryo transfer (MOET), artificial insemination, or in vitro production of embryos. This paper tries to discuss the current and future perspectives of cryopreservation of in vivo-and in vitro-produced sheep embryos concerning advantages and limitations for its practical use and possible solutions for improving methods to allow a higher survival rate of cryopreserved embryos.

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Section: Collection Of Oocytesmentioning

confidence: 99%

Section: Collection Of Oocytesmentioning

confidence: 99%

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

Section: In Vitro Culture (Ivc)mentioning

confidence: 99%

See 2 more Smart Citations

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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“…Reproductive performance including ovulation, fertilization, implantation, gestation and parturition is affected by environmental, health, management and genetic factors. Efforts are continuously paid to improve the fertility rates of sheep and goat through the assisted reproductive technologies (ART) such as estrus synchronization and diagnosis of early pregnancy diagnosis and gestational age by ultrasonography (Amiridis and Cseh, 2012). Estrus synchronization depends on either extending the luteal lifespan with exogenous progesterone (P4) or by inducing premature luteolysis using prostaglandin F2α (PGF2α) (Wildeus, 2000).…”

Section: Introductionmentioning

confidence: 99%

CIDR and Ultrasound-Fetometry Scan; Comparative Study Between Sheep and Goat in Egypt

Ahmed¹,

El-Naeim²,

Ibrahim³

2017

AJVS

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Key words:CIDR, CRL, BPD, FOD, Progesterone, PMSG, Ultrasound.The present study aims to evaluate the impact of controlled internal drug release (CIDR) in combination with prostaglandin F2α (PGF2α) and pregnant mare serum gonadotropin (PMSG) treatments on reproductive performance of dairy ewes and does besides, evaluation the accuracy of pregnancy diagnosis during the breeding season. Twenty female animals divided as Ossimi ewes (n=10) and Baladi does (n=10) were subjected to ovsynch regimen. Ultrasonographic examinations were conducted weekly using realtime B-mode ultrasound scanner by transrectal linear and transabdominal convex probes starting from day 14 post service. This study showed that estrus response was 100% in both ewes and does. The conception rates were 90% in ewes and 80 % in does. The incidence of twining was higher in ewes when compared to that in does with incidence rate 67% and 50 in ewes and does, respectively. The accuracy of ultrasound examination for determining fetal number was 100% for single and twin pregnancy. The birth rate was 100 % for both species; and this was confirmed at lambing / kidding. In conclusion, our results indicate that using biotechnologies such as ovsynch regimen and pregnancy diagnosis by ultrasonography are useful to improve the management of reproduction in ewes and does.

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