This paper contributes to the literature on social acceptance of artificial insemination and in-vitro fertilization, the two most prominent examples of Assisted Reproduction Technologies (ART) in Europe and direct attention to several previously under-researched aspects. The empirical base of this study is the fourth round of the European Values Study (EVS), conducted in 2008-2010 in 43 countries. Using multilevel fixed-effects linear regressions, we examine the impact of several individual and country-level characteristics on the agreement level with the statement that artificial insemination or in-vitro fertilization can always be justified, never be justified or something in between. According to our findings there are strong relationships between attitudes towards ART and socio-demographic variables, as well as religiousness and individual attitudes such as traditional family formation practices, "justification of homosexuality", (non-)preference for homosexual neighbours and acceptance of adoption by homosexual couples. As for the country level variables we found significant effect in case of those variables which are related to social changes within the concept Second Demographic Transitions such as mean age at first birth and secularization but GDP did not have any significant effects.