Assisted Reproductive Technologies (ART) Directed to Germplasm Preservation

Pereira, Rúben; Marques, C.C.; Pimenta, Jorge; Barbas, João Pedro; Baptista, M.C.; Diniz, P.; Torres, Ana; Lopes-da-Costa, L.

doi:10.1007/978-3-030-61981-7_10

Cited by 7 publications

(7 citation statements)

References 77 publications

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“…Sperm cryopreservation is an effective method used in the management and preservation of fertility of animals and humans through assisted reproduction techniques (ART) ( 128 , 129 ). This technique is based on the freezing of sperm to maintain its viability and functionality, and when performed correctly, it allows long-term freezing, as it results in the arrest of cellular metabolism that prevents cellular aging, maintaining viability and fertilization potential, an essential part for ART ( 130 ).…”

Section: Current Strategies To Reestablish Testicular Functionalitymentioning

confidence: 99%

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

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Traditional therapeutic interventions aim to restore male fertile potential or preserve sperm viability in severe cases, such as semen cryopreservation, testicular tissue, germ cell transplantation and testicular graft. However, these techniques demonstrate several methodological, clinical, and biological limitations, that impact in their results. In this scenario, reproductive medicine has sought biotechnological alternatives applied for infertility treatment, or to improve gamete preservation and thus increase reproductive rates in vitro and in vivo. One of the main approaches employed is the biomimetic testicular tissue reconstruction, which uses tissue-engineering principles and methodologies. This strategy pursues to mimic the testicular microenvironment, simulating physiological conditions. Such approach allows male gametes maintenance in culture or produce viable grafts that can be transplanted and restore reproductive functions. In this context, the application of several biomaterials have been proposed to be used in artificial biological systems. From synthetic polymers to decellularized matrixes, each biomaterial has advantages and disadvantages regarding its application in cell culture and tissue reconstruction. Therefore, the present review aims to list the progress that has been made and the continued challenges facing testicular regenerative medicine and the preservation of male reproductive capacity, based on the development of tissue bioengineering approaches for testicular tissue microenvironment reconstruction.

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Section: Current Strategies To Reestablish Testicular Functionalitymentioning

confidence: 99%

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

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“…Profiling the proteome of reproductive tissues under various conditions can identify the protein markers associated with poor reproductive performance, providing valuable insights into how external factors influence fertility and reproductive success in chickens [ 40 ]. The field of assisted reproductive technologies (ARTs), including artificial insemination and embryo modification, has also been affected by the application of proteomics in chicken reproduction [ 41 ]. The characterization of the proteome profiles of gametes, embryos, and reproductive organs can improve the success rates of assisted reproduction in chickens, refine ART procedures, and increase the effectiveness of fertility therapies [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

“…The field of assisted reproductive technologies (ARTs), including artificial insemination and embryo modification, has also been affected by the application of proteomics in chicken reproduction [ 41 ]. The characterization of the proteome profiles of gametes, embryos, and reproductive organs can improve the success rates of assisted reproduction in chickens, refine ART procedures, and increase the effectiveness of fertility therapies [ 41 ]. Similarly, proteomics can also be used to identify the biomarkers associated with egg quality, such as proteins that are involved in the formation of the eggshell or proteins that are indicative of the nutritional quality of the egg.…”

Section: Introductionmentioning

confidence: 99%

The Omics Revolution in Understanding Chicken Reproduction: A Comprehensive Review

Wadood,

Zhang

2024

CIMB

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Omics approaches have significantly contributed to our understanding of several aspects of chicken reproduction. This review paper gives an overview of the use of omics technologies such as genomics, transcriptomics, proteomics, and metabolomics to elucidate the mechanisms of chicken reproduction. Genomics has transformed the study of chicken reproduction by allowing the examination of the full genetic makeup of chickens, resulting in the discovery of genes associated with reproductive features and disorders. Transcriptomics has provided insights into the gene expression patterns and regulatory mechanisms involved in reproductive processes, allowing for a better knowledge of developmental stages and hormone regulation. Furthermore, proteomics has made it easier to identify and quantify the proteins involved in reproductive physiology to better understand the molecular mechanisms driving fertility, embryonic development, and egg quality. Metabolomics has emerged as a useful technique for understanding the metabolic pathways and biomarkers linked to reproductive performance, providing vital insights for enhancing breeding tactics and reproductive health. The integration of omics data has resulted in the identification of critical molecular pathways and biomarkers linked with chicken reproductive features, providing the opportunity for targeted genetic selection and improved reproductive management approaches. Furthermore, omics technologies have helped to create biomarkers for fertility and embryonic viability, providing the poultry sector with tools for effective breeding and reproductive health management. Finally, omics technologies have greatly improved our understanding of chicken reproduction by revealing the molecular complexities that underpin reproductive processes.

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“…In the National Zootechnical Station (EZN, Portugal), the first experiments on ram semen cryopreservation using animals of native breeds (Serra da Estrela, Merino Branco and Saloia) were performed in 1998 [2,9]. After this date and until now, several other local breeds have been evaluated and their semen incorporated into the Portuguese Animal Germplasm Bank (BPGA) for ex situ conservation purposes [14]. An increase in the number and size of germplasm banks to face the significant decrease of biodiversity due to the rise of endangered and or extinction of domestic breeds, has been reported in the last 10 years [14].…”

Section: Introductionmentioning

confidence: 99%

“…After this date and until now, several other local breeds have been evaluated and their semen incorporated into the Portuguese Animal Germplasm Bank (BPGA) for ex situ conservation purposes [14]. An increase in the number and size of germplasm banks to face the significant decrease of biodiversity due to the rise of endangered and or extinction of domestic breeds, has been reported in the last 10 years [14].…”

Section: Introductionmentioning

confidence: 99%

Ram Semen Cryopreservation for Portuguese Native Breeds: Season and Breed Effects on Semen Quality Variation

Barbas

Pimenta

Baptista

et al. 2023

Animals

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The semen quality is one of the determinant factors of ram semen cryopreservation. The present retrospective study aimed to characterize the seasonal ram pattern during the year for ten Portuguese local sheep breeds, hypothesizing that the breed and season had low effects on the main spermatozoa traits. A total of 1471 ejaculates were used and evaluated (fresh semen) from 85 rams between 2004 and 2020 and re-evaluated after thawing (thawed semen). The effect of breed, season, and sperm cryopreservation on nine semen traits were evaluated. The volume per ejaculate, spermatozoa (SPZ) concentration, and total number of SPZ per ejaculate, were affected by breed (p < 0.001) but not by season (p > 0.05). As expected, the semen processing was the most significant (p < 0.001) factor of variation on seminal parameters. Moreover, breed and interactions between breed × semen processing, modulated the response of alive SPZ, abnormal morphology, head, and intermediate piece defects. In fresh semen, season only affected the intermediate piece defects due to the highest percentage observed between February and April period in some breeds. Overall, and despite the mentioned particularities, there were similarities among the ten local breeds. We also concluded that the seasonal effect on ejaculate and SPZ traits is not significant in our region. These local ram breeds have low seasonality and can be employed in natural mating as well as semen donors for cryopreservation and assisted reproductive biotechnologies during the whole year at our latitude.

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Assisted Reproductive Technologies (ART) Directed to Germplasm Preservation

Cited by 7 publications

References 77 publications

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

The Omics Revolution in Understanding Chicken Reproduction: A Comprehensive Review

Ram Semen Cryopreservation for Portuguese Native Breeds: Season and Breed Effects on Semen Quality Variation

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