Abstract:Animal experiments are very important for the development of new assisted reproductive techniques (ART) for use in human and animal reproductive medicine. Most technical aspects of reproductive manipulation of humans and animals are very similar, and many components of successful human ART used nowadays have been derived from animal studies. In this study we examined (1) the use of 'non-contact' laser for assisted hatching, (2) whether spindles in living mouse oocytes could safely be imaged/examined by polaris… Show more
“…In vitro fertilization with ICSI was performed 3-4 h after thawing, and fertilization was assessed 12-16 h later. Assisted hatching (AH) with laser was performed on all embryos prior to transfer [13]. Embryo transfer was carried out 48-72 h after ICSI at the 2 to 8 cell stages.…”
“…In vitro fertilization with ICSI was performed 3-4 h after thawing, and fertilization was assessed 12-16 h later. Assisted hatching (AH) with laser was performed on all embryos prior to transfer [13]. Embryo transfer was carried out 48-72 h after ICSI at the 2 to 8 cell stages.…”
“…Up to three embryos were selected for transfer into the uterine cavity. Prior to ET laser assisted hatching was performed on each of the embryos (MTG, Fertilase, Hamburg, Germany) [11]. Pregnancy was defined as a spontaneous rise in a βhCG concentration at least 10 days post-transfer.…”
State/condition of injected testicular spermatozoa has impact to fertilization and embryo development. Pregnancy/delivery can be achieved with frozen/immotile spermatozoa.
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