Assisted Fertilization and Nuclear Transfer in Nonhuman Primates

Ouhibi, Nadia; Zelinski-Wooten, Mary B.; Thomson, James A.; Wolf, Don P.

doi:10.1007/978-1-59259-369-9_16

Cited by 15 publications

(11 citation statements)

References 111 publications

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“…The pregnancy established in this study occurred after the transfer of 30 NT embryos derived from 8-to 16-cell stage blastomeres into 11 recipients. This pregnancy rate of 9% is significantly lower than that observed following the oviductal transfer of in vitro fertilization-produced intact embryos (66%) in our laboratory [38].…”

Section: Discussioncontrasting

confidence: 59%

Rhesus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells1

Mitalipov

Yeoman

Nusser

et al. 2002

Biology of Reproduction

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Production of genetically identical nonhuman primates would reduce the number of animals required for biomedical research and dramatically impact studies pertaining to immune system function, such as development of the human-immunodeficiency-virus vaccine. Our long-term goal is to develop robust somatic cell cloning and/or twinning protocols in the rhesus macaque. The objective of this study was to determine the developmental competence of nuclear transfer (NT) embryos derived from embryonic blastomeres (embryonic cell NT) or fetal fibroblasts (somatic cell NT) as a first step in the production of rhesus monkeys by somatic cell cloning. Development of cleaved embryos up to the 8-cell stage was similar among embryonic and somatic cell NT embryos and comparable to controls created by intracytoplasmic sperm injection (ICSI; mean +/- SEM, 81 +/- 5%, 88 +/- 7%, and 87 +/- 4%, respectively). However, significantly lower rates of development to the blastocyst stage were observed with somatic cell NT embryos (1%) in contrast to embryonic cell NT (34 +/- 15%) or ICSI control embryos (46 +/- 6%). Development of somatic cell NT embryos was not markedly affected by donor cell treatment, timing of activation, or chemical activation protocol. Transfer of embryonic, but not of somatic cell NT embryos, into recipients resulted in term pregnancy. Future efforts will focus on optimizing the production of somatic cell NT embryos that develop in high efficiency to the blastocyst stage in vitro.

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Section: Discussioncontrasting

confidence: 59%

Rhesus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells1

Mitalipov

Yeoman

Nusser

et al. 2002

Biology of Reproduction

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“…Unlike in many fields where knowledge flows from non-human primate (NHP) research to clinical application in humans, the development of the ARTs in monkeys historically postdates human application. The experience in NHP, however, while nowhere nearly as robust as that in humans has been associated with several landmark accomplishments and methods for establishing pregnancies are currently available as summarized by us in 2001 [1]. While a few reports in New World monkeys and Great Apes exist, most research efforts have focused on Old World macaques with the rhesus monkey the primary subject and that species is the focus of this review.…”

Section: Reviewmentioning

confidence: 99%

“…This chapter will summarize the experience at ONPRC with the ARTs in the rhesus monkey building on two previous reviews [1,7]. The methodology employed in the conduction of the ARTs in monkeys can also be found in these reviews.…”

Section: Reviewmentioning

confidence: 99%

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Assisted reproductive technologies in rhesus macaques

Wolf

2004

Reprod Biol Endocrinol

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The assisted reproductive technologies (ARTs) have been used in the production of rhesus monkey offspring at the Oregon National Primate Research Center (ONPRC) and that experience is summarized here. Additionally these technologies serve as a source of oocytes/embryos for monozygotic twinning, embryonic stem (ES) cell derivation and cloning. High fertilization efficiencies were realized with conventional insemination or following the use of intracytoplasmic sperm injection (ICSI) and approximately 50% of the resulting embryos grew in vitro to blastocysts. Both fresh and frozen sperm were employed in fertilization by ICSI and the resulting embryos could be low temperature stored for subsequent thawing and transfer when a synchronized recipient female was available or after shipment to another facility. Following the transfer of up to 3 embryos, an overall pregnancy rate of 30% was achieved with increasing rates dependent upon the number of embryos transferred. Singleton pregnancy outcomes following the transfer of ART produced embryos were similar to those observed in a control group of animals in the timed mated breeding colony at ONPRC. ICSI produced embryos were used in efforts to create monozygotic twins by blastomere separation or blastocyst splitting. While pregnancies were achieved following the transfer of demi-embryos, only one was a twin and it was lost to spontaneous abortion. ICSI produced embryos have also served as the source of blastocysts for the derivation of embryonic stem cells. These pluripotent cells hold potential for cell based therapies and we consider the monkey an important translational model in which to evaluate safety, efficacy and feasibility of regenerative medicine approaches based on the transplantation of stem cell-derived progeny. Finally, efforts to produce genetically-identical monkeys by nuclear transfer have been briefly summarized.

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“…Os oócitos foram obtidos por meio de videolaparoscopia após estimulação ovariana controlada, seguindo protocolo adaptado a partir de trabalhos anteriores (ZELINSKI-WOOTEN et al, 1995;OUHIBI et al, 2001 BAVISTER et al, 1984) antes da inseminação.…”

Section: Colheita Avaliação E Preparo Dos Oócitosunclassified

<b>Criopreservação de sêmen de primatas não-humanos</b>

Carvalho¹

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Aos meus irmãos, Mateus e Paulo, pelos conselhos e por sempre "estarem lá" À minha grande família, incluindo tios, tias, primos e primas, pelo apoio incondicional e reuniões descontraídas.Aos meus amigos e amigas que, de longe ou de perto, sempre fizeram a diferença.Ao meu coorientador e amigo, Prof. Rodrigo R. Valle, por ter me incentivado e me ajudado a entrar na pós-graduação; por ter me introduzido à área de À técnica e amiga Cathy Ramsey, do ART Core, que me ajudou durante toda a realização do meu projeto da BEPE.À pesquisadora, Dra. Carol Hanna, que atuou como co-supervisora do meu projeto da BEPE, contribuindo tanto na elaboração como na execução do projeto.Aos demais integrantes do Hennebold Lab, Melinda, Nathan, Jill e Alison, pela ajuda e convivência.À pesquisadora e amiga, Dra. Naida Loskutoff (in memoriam) do Omaha's Henry Doorly Zoo, que também nos deixou precocemente no último ano, pelo fornecimento do corante eosina-fast green. Às instituiçõesAgradeço a todas as instituições que tornaram possível a realização dos meus estudos e pesquisas. No Brasil À Faculdade de Medicina Veterinária e Zootecnia da Universidade de SãoPaulo (FMVZ/USP) por fornecer toda a estrutura técnico-científica para realização do meu Doutorado. Primate Research Center (OHSU) por fornecer toda a estrutura e apoio técnico e financeiro para realização do meu projeto de pesquisa no exterior. Aos AnimaisAgradeço também, aos primatas não-humanos, nossos passivos e inocentes parceiros dessa jornada. better results when compared to vitrification (p<0,05). SW had better results, followed by DGC and SU, while GWF had the worse results. SW and DGC were further evaluated by IVF. Fertilization rates (mean±SEM%) with MI oocytes using fresh sperm were significantly higher (43.5±16.4) than with SW (2.0±2.0) and did not differ from DGC (25.1±14.2). There was no difference in blastocyst rates between treatments (range 0 to 11.9±7.9). Fertilization rates with MII ova were also significantly higher with fresh sperm (41.4±3.6) than DGC and SW (18.4±6.9 and 12.7±7.7, respectively), and more blastocysts developed from MIIs fertilized with fresh sperm (64.7±13.6) than SW and DGC (4.7±4.7 and 30.9±13.8, respectively). In conclusion, frozen-thawed sperm were able to fertilize oocytes and embryos reached the blastocyst stage. DGC yielded better frozen-thawed sperm for IVF in rhesus macaques, when compared with SW.

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Assisted Fertilization and Nuclear Transfer in Nonhuman Primates

Cited by 15 publications

References 111 publications

Rhesus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells1

Rhesus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells1

Assisted reproductive technologies in rhesus macaques

<b>Criopreservação de sêmen de primatas não-humanos</b>

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