Assimilatory Nitrate Reduction in Hansenula polymorpha

Rossi, Brunella; Berardi, Enrico

doi:10.1007/978-1-4020-8292-4_15

Cited by 2 publications

(1 citation statement)

References 49 publications

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“…While the basic regulatory circuits in fungi were identified more than 30 years ago, the precise molecular mechanisms responsible for control of fungal nitrate growth have been described only recently (Marzluf, ; Wong et al ., ). The current information on nitrate utilization gene regulation in fungi comes almost entirely from studies on saprotrophic and pathogenic ascomycete species such as Aspergillus nidulans , Neurospora crassa and Magnaporthe grisea , and on the ascomycete yeast Hansenula polymorpha (Crawford and Arst, ; Marzluf, ; Tao and Marzluf, ; Siverio, ; Wong et al ., ; Rossi and Berardi, ). Unfortunately, the information on genetic control of nitrate growth in other taxa, such as basidiomycetes, is minimal.…”

Section: Introductionmentioning

confidence: 98%

LbNrt RNA silencing in the mycorrhizal symbiont Laccaria bicolor reveals a nitrate‐independent regulatory role for a eukaryotic NRT2‐type nitrate transporter

Kemppainen

Pardo

2013

Environ Microbiol Rep

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Fungal nitrogen metabolism plays a fundamental role in function of mycorrhizal symbiosis and consequently in nutrient cycling of terrestrial ecosystems. Despite its global ecological relevance the information on control and molecular regulation of nitrogen utilization in mycorrhizal fungi is very limited. We have extended the nitrate utilization RNA silencing studies of the model mycorrhizal basidiomycete, Laccaria bicolor, by altering the expression of LbNrt, the sole nitrate transporter-encoding gene of the fungus. Here we report the first nutrient transporter mutants for mycorrhizal fungi. Silencing of LbNrt results in fungal strains with minimal detectable LbNrt transcript levels, significantly reduced growth capacity on nitrate and altered symbiotic interaction with poplar. Transporter silencing also creates marked co-downregulation of whole Laccaria fHANT-AC (fungal high-affinity nitrate assimilation cluster). Most importantly, this effect on the nitrate utilization pathway appears independent of extracellular nitrate or nitrogen status of the fungus. Our results indicate a novel and central nitrate uptake-independent regulatory role for a eukaryotic nitrate transporter. The possible cellular mechanisms behind this regulation mode are discussed in the light of current knowledge on NRT2-type nitrate transporters in different eukaryotes.

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Section: Introductionmentioning

confidence: 98%

LbNrt RNA silencing in the mycorrhizal symbiont Laccaria bicolor reveals a nitrate‐independent regulatory role for a eukaryotic NRT2‐type nitrate transporter

Kemppainen

Pardo

2013

Environ Microbiol Rep

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Insights into the Dekkera bruxellensis Genomic Landscape: Comparative Genomics Reveals Variations in Ploidy and Nutrient Utilisation Potential amongst Wine Isolates

et al. 2014

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The yeast Dekkera bruxellensis is a major contaminant of industrial fermentations, such as those used for the production of biofuel and wine, where it outlasts and, under some conditions, outcompetes the major industrial yeast Saccharomyces cerevisiae. In order to investigate the level of inter-strain variation that is present within this economically important species, the genomes of four diverse D. bruxellensis isolates were compared. While each of the four strains was shown to contain a core diploid genome, which is clearly sufficient for survival, two of the four isolates have a third haploid complement of chromosomes. The sequences of these additional haploid genomes were both highly divergent from those comprising the diploid core and divergent between the two triploid strains. Similar to examples in the Saccharomyces spp. clade, where some allotriploids have arisen on the basis of enhanced ability to survive a range of environmental conditions, it is likely these strains are products of two independent hybridisation events that may have involved multiple species or distinct sub-species of Dekkera. Interestingly these triploid strains represent the vast majority (92%) of isolates from across the Australian wine industry, suggesting that the additional set of chromosomes may confer a selective advantage in winery environments that has resulted in these hybrid strains all-but replacing their diploid counterparts in Australian winery settings. In addition to the apparent inter-specific hybridisation events, chromosomal aberrations such as strain-specific insertions and deletions and loss-of-heterozygosity by gene conversion were also commonplace. While these events are likely to have affected many phenotypes across these strains, we have been able to link a specific deletion to the inability to utilise nitrate by some strains of D. bruxellensis, a phenotype that may have direct impacts in the ability for these strains to compete with S. cerevisiae.

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Assimilatory Nitrate Reduction in Hansenula polymorpha

Cited by 2 publications

References 49 publications

LbNrt RNA silencing in the mycorrhizal symbiont Laccaria bicolor reveals a nitrate‐independent regulatory role for a eukaryotic NRT2‐type nitrate transporter

LbNrt RNA silencing in the mycorrhizal symbiont Laccaria bicolor reveals a nitrate‐independent regulatory role for a eukaryotic NRT2‐type nitrate transporter

Insights into the Dekkera bruxellensis Genomic Landscape: Comparative Genomics Reveals Variations in Ploidy and Nutrient Utilisation Potential amongst Wine Isolates

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