Assimilatory Nitrate-Nitrite Reduction

Hewitt, E. J.

doi:10.1146/annurev.pp.26.060175.000445

Cited by 289 publications

(84 citation statements)

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“…It is considered that nitrate reductase, nitrite reductase, glutamine synthetase, and GOGAT are involved in such activities, as discussed in several recent reviews (3,4,19). Among these enzymes, nitrate reductase activity has been most extensively studied by many workers.…”

Section: Discussionmentioning

confidence: 99%

Effects of Supplemental Nitrate Application on the Activity of Some Nitrogen Assimilation Enzymes and Leaf Tissue Productivity in Maize Seedlings

Watanabe

Hayashi

Sugiyama

1985

Soil Science and Plant Nutrition

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Section: Discussionmentioning

confidence: 99%

Effects of Supplemental Nitrate Application on the Activity of Some Nitrogen Assimilation Enzymes and Leaf Tissue Productivity in Maize Seedlings

Watanabe

Hayashi

Sugiyama

1985

Soil Science and Plant Nutrition

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“…Assimilatory NR3 is generally recognized as the catalyst for the initial step ofnitrate assimilation where nitrate is reduced to nitrite with pyridine nucleotide as electron donor (10). Assimilatory NR are accepted to be of large molecular size, and to contain hemeiron, flavin-adenine dinucleotide, and molybdenum.…”

mentioning

confidence: 99%

Immunological Approach to Structural Comparisons of Assimilatory Nitrate Reductases

John

Campbell²

1981

Plant Physiol.

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Homogeneous squash cotyledon reduced nicotinamide-adenine dinucleotide (NADH):nitrate reductase (NR) was isolated using blue-Sepharose and polyacrylamide gel electrophoresis. Gel slices containing NR were pulverized and injected into a previously unimmunized rabbit. This process was repeated weekly and antiserum to NR was obtained after four weeks.Analysis of the antiserum by Ouchterlony double diffusion using a blueSepharose preparation of NR resulted in a single precipitin band while immunoelectrophoresis revealed two minor contaminants. The antiserum was found to inhibit the NR reaction and the partial reactions to different degrees. When the NADH:NR and the reduced methyl viologen:NR activities were inhibited 90% by specifically diluted antiserum, the reduction of cytochrome c was inhibited 50%, and the reduction of ferricyanide was inhibited only 30%. Antiserum was also used to compare the cross reactivides of NR from squash cotyledons, spinach, corn, and soybean leaves, Chloregla vulgaris, and Neurospora crassa. These tests revealed a high degree of shnilarity between NADH:NR from the squash and spinach, while NADH:NR from corn and soybean and the NAD(P)H:NR from soybean were less closely related to the squash NADH:NR. The green algal (C. vulgaris) NADH:NR and the fungal (N. crassa) NADPH:NR were very low in cross reactivity and are apparently quite different from squash NADH:NR in antigenicity. Antiserum to N. crassa NADPH:NR failed to give a positive Ouchterlony result with higher plant or C. vulgaris NADH: NR, but this antiserum did inhibit the activity of squash NR. Thus, it can be concluded from these immunological comparisons that all seven forms of assimilatory NR studied here have antigenic determinants in common and are probably derived from a common ancestor. Although these assimilatory NR have similar catalytic characteristics, they appear to have diverged to a great degree in their structural features.Assimilatory NR3 is generally recognized as the catalyst for the initial step ofnitrate assimilation where nitrate is reduced to nitrite with pyridine nucleotide as electron donor (10). Assimilatory NR are accepted to be of large molecular size, and to contain hemeiron, flavin-adenine dinucleotide, and molybdenum. These components act as electron carriers between the NADH oxidation site and the nitrate reduction site (5). NR is also capable of acting as an NADH dehydrogenase, catalyzing the reduction of ferricya-

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“…Affinity chromatography has been developed for these flavoproteins taking advantage of these characteristics [18,19]. The nature of binding of the enzyme to ferredoxin-Sepharose is not clear, but the affinity between NiR and ferredoxin, described for the first time here, is understandable because of a pivotal role of ferredoxin as reducing agent in the photosynthetic apparatus [20] from which electrons are derived for nitrite reduction [2]. These findings provide additonal interests in enzymological and physiological aspects of NiR.…”

Section: Resultsmentioning

confidence: 99%

“…and yields nitrate, and the subsequent step is catalyzed by nitrite reductase (ferredoxin-nitrite oxidoreductase, EC 1.6.6.4) to yield ammonia with the use of six electrons [1,2]. Recently nitrate reductase has been obtained in a highly purified state by the use of affinity chromatography in which FAD [3], Blue dextran [4], NADH [5], and p-nitroaniline, respectively [6] were used as specific or general ligands coupled to Sepharose gels.…”

Section: Introductionmentioning

confidence: 99%

Ferredoxin‐sepharose affinity chromatography for the purification of assimilatory nitrite reductase

1976

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Assimilatory Nitrate-Nitrite Reduction

Cited by 289 publications

References 0 publications

Effects of Supplemental Nitrate Application on the Activity of Some Nitrogen Assimilation Enzymes and Leaf Tissue Productivity in Maize Seedlings

Effects of Supplemental Nitrate Application on the Activity of Some Nitrogen Assimilation Enzymes and Leaf Tissue Productivity in Maize Seedlings

Immunological Approach to Structural Comparisons of Assimilatory Nitrate Reductases

Ferredoxin‐sepharose affinity chromatography for the purification of assimilatory nitrite reductase

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