Assimilation and transport of nitrate, nitrite, and ammonia absorbed by nodulated soybean plants

Ohyama, Takuji; Saito, Keiko; Kato, Naoki

doi:10.1080/00380768.1989.10434732

Cited by 11 publications

(5 citation statements)

References 14 publications

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“…In our experiment, greater NH4 + incorporation (almost double) than NO3was shown within 48 hours after treatment into soybean roots (Fig. 3), consistent with a study by Ohyama et al (1989). In soybean, NH4 + is actively incorporated into amides, much of which remains in the roots (Weissman, 1972).…”

Section: Physiological Mechanisms Of N Preferencesupporting

confidence: 92%

Nitrogen preference across generations under changing ammonium nitrate ratios

Daryanto

Wang

Gilhooly

et al. 2018

Journal of Plant Ecology

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Aims: Nitrogen (N) in natural environments is typically supplied by a mixture of ammonia (NH4 +) and nitrate (NO3-). However, factors that underlie either NH4 + or NO3preference, and how such preference will change across generations remain unclear. We conducted a series of experiments to answer whether: (i) NH4 + :NO3ratio is the driving factor for plant N preference, and (ii) this preference is consistent across generations. Methods: We conducted both: (i) field observations (as a proxy for parent or P generation) and (ii) greenhouse experiments (the first generation or F1 and the second generation or F2) using corn and soybean grown under different NH4 + :NO3ratios. Important findings: Both corn and soybean had the physiological plasticity to prefer either NH4 + or NO3depending on NH4 + :NO3ratios, and this plasticity was consistent across generations. Corn, however, showed a stronger preference towards NO3while soybean showed a stronger preference towards NH4 +. While both plants would try to make use of the most available form of N in their growing medium, plant species, physiological characteristics (e.g., maturity) and plant nutrient status also determined the extent of N uptake. From the evolutionary and productivity perspective, this plasticity is beneficial, allowing plants to effectively acquire available N particularly in a changing climate.

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Section: Physiological Mechanisms Of N Preferencesupporting

confidence: 92%

Nitrogen preference across generations under changing ammonium nitrate ratios

Daryanto

Wang

Gilhooly

et al. 2018

Journal of Plant Ecology

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“…These results suggested that the transport rates from source organs, either the nodules or the roots, were similar, but fixed-N was more rapidly transported to the pods and seeds; also the absorbed nitrate was highly distributed in the leaves. The nodulated soybean plants were treated with a culture solution containing 15 NO 3 - , 15 NO 2 - , or 15 NH 4 + , and the absorption and transport of N were investigated (Ohyama et al, 1989b). After 24 h of 15 N-supply, the amount and distribution of 15 N among the roots, nodules, stems, and leaves were very similar between 15 NO 3 - and 15 NH 4 + , although the absorption and transport to the shoot from 15 NO 2 - was much lower than that from 15 NO 3 - and 15 NH 4 + and most of the 15 N from the 15 NO 2 - remained in the roots.…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Chemical Forms of Nitrogen on the Quick and Reversible Inhibition of Soybean Nodule Growth and Nitrogen Fixation Activity

et al. 2019

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It has been reported that supply of nitrate to culture solution rapidly and reversibly inhibits nodule growth and nitrogen fixation activity of soybean. In this study, the effects of ammonium, urea, or glutamine on nodule growth and nitrogen fixation activity are compared with that for nitrate. Soybean plants were cultivated with a nitrogen-free nutrient solution, then 1 mM-N of nitrate, ammonium, glutamine, or urea were supplied from 12 DAP until 17 DAP. Repression of nodule growth and nitrogen fixation activity at 17 DAP were observed by ammonium, urea, and glutamine like nitrate, although the inhibitory effects were milder than nitrate. The removal of nitrogen from the culture solutions after nitrogen treatments resulted in a recovery of the nodule growth. It was found that the glutamine treatment followed by N-free cultivation gave highest nitrogen fixation activity about two times of the control. Tracer experiments with 15 N and 13 C were performed to evaluate the translocation of N and C to the different tissues. Culture solutions containing a 15 N-labeled nitrogen source were supplied from 21 DAP, and the whole shoots were exposed to 13 CO 2 for 60 min on 23 DAP, and plants were harvested on 24 DAP. The percentage distribution of 15 N in nodules was highest for ammonium (1.4%) followed by glutamine (0.78%), urea (0.32%) and nitrate (0.25%). The percentage distribution of 13 C in the nodules was highest for the control (11.5%) followed by urea (5.8%), glutamine (2.6%), ammonium (2.3%), and nitrate (2.3%). The inhibitory effects of nitrogen compounds appeared to be related to a decrease in photoassimilate partitioning in the nodules, rather than 15 N transport into the nodules. The free amino acid concentrations after nitrogen treatments were increased in the nodules and leaves by nitrate, in the roots by ammonium, in the stems by urea, and the roots, stems, and leaves by glutamine treatment. The concentrations of asparagine, aspartate, and glutamine were increased after nitrogen treatments. By the long-term supply of nitrogen for 2-weeks, nitrate significantly increased the lateral roots and leaf growth. The long-term supply of urea and glutamine also promoted the lateral roots and leaf growth, but ammonium suppressed them.

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“…In order to compare the assimilation and transport of nitrate, nitrite, and ammonium, 15 [23]. The N from nitrate and ammonium was rapidly absorbed, and 70% of 15 N absorbed was distributed in the shoots at 24 hours after 15 N treatments.…”

Section: Amino Acid Metabolism In Soybean Rootsmentioning

confidence: 99%

Amino Acid Metabolism and Transport in Soybean Plants

Ohyama¹,

Ohtake²,

Sueyoshi³

et al. 2017

Amino Acid - New Insights and Roles in Plant and Animal

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The ammonium produced by nitrogen fixation in the bacteroid is rapidly excreted to cytosol of infected cell of soybean nodules and then assimilated into glutamine and glutamic acid, by glutamine synthetase/glutamate synthase pathway. Most of the nitrogen is further assimilated into ureides, allantoin, and allantoic acid, via purine synthesis, and they are transported through xylem to the shoots. Nitrate absorbed in the roots is reduced by nitrate reductase and nitrite reductase to ammonia either in the roots or leaves. The ammonia is also assimilated by glutamine synthetase/glutamate synthase pathway, and mainly transported by asparagine, and not ureides. The nitrogen transported into leaves is readily utilized for protein synthesis, and then, some of them are decomposed and retransported to roots, apical shoots, and pods via phloem mainly in the form of asparagine.

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Assimilation and transport of nitrate, nitrite, and ammonia absorbed by nodulated soybean plants

Cited by 11 publications

References 14 publications

Nitrogen preference across generations under changing ammonium nitrate ratios

Nitrogen preference across generations under changing ammonium nitrate ratios

Effects of Different Chemical Forms of Nitrogen on the Quick and Reversible Inhibition of Soybean Nodule Growth and Nitrogen Fixation Activity

Amino Acid Metabolism and Transport in Soybean Plants

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