Assignment of the mouse alpha A-crystallin structural gene to chromosome 17

Kaye, Nancy Weber; Church, Robert L.; Piatigorsky, Joram; Petrash, J. Mark; Lalley, Peter A.

doi:10.3109/02713688509017685

Cited by 8 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The murine aAcrystallin gene is located on chromosome 17 (11,12) and codes for two polypeptides, aA2 and aAin8, that are produced by alternative RNA splicing (8). These genes are situated on different chromosomes in the human (5).…”

Section: Activation Of the Murine Ala-crystallin Gene Promoter In Expmentioning

confidence: 99%

Crystallin Genes: Lens Specificity of the Murine aA-Crystallin Gene

Chepelinsky¹,

Khillan²,

Mahon³

et al. 1987

Environmental Health Perspectives

Self Cite

View full text Add to dashboard Cite

The abundant soluble proteins of the eye lens, the crystallins, are encoded by several gene families which are developmentally regulated in the embryonic lens. We have studied the expression of the murine alpha A-crystallin gene. Transfection experiments using the pSVO-CAT vector and explanted lens epithelia from embryonic chickens demonstrated proximal (-88 to -60) and distal (-111 to -85) regulatory sequences which interact when the alpha A-crystallin promoter is activated in the lens cells. Transgenic mouse experiments showed that the sequence between positions -366 to +46 of the alpha A-crystallin promoter can drive foreign genes selectively in the lens. A fusion gene consisting of this alpha A-crystallin promoter sequence and the T-antigen gene of SV40 produced a lens tumor in transgenic mice. Thus, crystallin promoters provide a useful model for tissue-specific gene expression and permit targeting the expression of foreign genes to a highly differentiated tissue during development.

show abstract

Section: Activation Of the Murine Ala-crystallin Gene Promoter In Expmentioning

confidence: 99%

Crystallin Genes: Lens Specificity of the Murine aA-Crystallin Gene

Chepelinsky¹,

Khillan²,

Mahon³

et al. 1987

Environmental Health Perspectives

Self Cite

View full text Add to dashboard Cite

show abstract

“…These genes are situated on different chromosomes in the human (5). The murine oaAcrystallin gene is located on chromosome 17 (11,12) and codes for two polypeptides, aA2 and aAm', that are produced by alternative RNA splicing (8). The splicing process that eliminates the insert exon occurs with higher efficiency.…”

Section: Introductionmentioning

confidence: 99%

Crystallin genes: lens specificity of the murine alpha A-crystallin gene.

Chepelinsky

Khillan

Mahon

et al. 1987

Environ Health Perspect

View full text Add to dashboard Cite

The abundant soluble proteins of the eye lens, the crystallins, are encoded by several gene families which are developmentally regulated in the embryonic lens. We have studied the expression of the murine aA-crystallin gene. Transfection experiments using the pSVO-CAT vector and explanted lens epithelia from embryonic chickens demonstrated proximal (-88 to -60) and distal (-111 to -85) regulatory sequences which interact when the aA-crystallin promoter is activated in the lens cells. Transgenic mouse experiments showed that the sequence between positions -366 to + 46 of the aA-crystallin promoter can drive foreign genes selectively in the lens. A fusion gene consisting of this aA-crystallin promoter sequence and the T-antigen gene of SV40 produced a lens tumor in transgenic mice. Thus, crystallin promoters provide a useful model for tissue-specific gene expression and permit targeting the expression of foreign genes to a highly differentiated tissue during development.

show abstract