Assignment of the Human Mitochondrial NADP+-Specific Isocitrate Dehydrogenase (IDH2) Gene to 15q26.1 byin SituHybridization

IDH mutations in acute myeloid leukemia

“…The human NADP-dependent IDH1 enzyme is encoded by IDH1 located at chromosome band 2q33.3 [26] and is localized in cytoplasm and peroxisomes [27]. The mitochondrial NADP-dependent IDH2 enzyme is encoded by IDH2 situated at chromosome band 15q26.1 [28]. Because mitochondrial NADH operates in energy metabolism, as a part of the tricarboxylic acid cycle, and cytosolic NADPH functions in anabolic processes and redox control, it is reasonable to expect the changes in 1 or more of these processes in tumors that carry an IDH1 or IDH2 mutation.…”

Silencing of cytosolic NADP+-dependent isocitrate dehydrogenase gene enhances ethanol-induced toxicity in HepG2 cells

“…Although the relationship between IDPc and its mitochondrial counterpart is unknown, it is likely that these two NADP + -dependent isoenzymes are derived from different genes, based on the lack of crossimmunoreactivity (Jennings and Stevenson, 1991), different chromosomal loci and gene sequences (Huh et al, 1993;Oh et al, 1996), different tissue-specific expression, and different subcellular localizations (Plaut et al, 1983). G6PDH has long been regarded as the major enzyme to generate cytosolic NADPH; however, we recently reported that IDPc also plays an essential role in supplying NADPH to the cytosol (Lee et al, 2002).…”

Molecular Markers for Risk Stratification in Adult Acute Myeloid Leukemia with Normal Cytogenetics

“…Three classes of IDH isoenzymes exist in mammalian cells (two forms of mitochondrial IDH and cytosolic IDH). IDH1 gene is localized to chromosome band 2q33.3 and IDH2 gene to chromosome band 15q26.1 (Narahara et al,1985;Oh et al,1996). IDH2 encodes the mitochondrial isoform that uses nicotinamide adenine dinucleotide phosphate as a cofactor.…”