Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10

Schonk, D.; Kuijpers, Helma J.H.; Drunen, Ellen van; Dalen, C. H. van; Kessel, A.H.M. Geurts van; Verheijen, R.; Ramaekers, F.

doi:10.1007/bf00285178

Cited by 97 publications

(65 citation statements)

References 13 publications

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“…Only AA/E7 showed suprabasal epithelial layers consisting of highly keratinized and atypical cells. Ki67, a marker to determine the growth fraction of a given cell population (Schonk et al, 1989) was observed in single cells within the lower, and interestingly, also the upper (keratinizing) part of the AA/E7 raft epithelium, as well as in single cells of the E6/E7 raft epithelium (Figure 4b). Although differentiation-associated cytokeratin 10 (K10), was found in the majority of suprabasal cells within the AA/E7 culture, the E6/E7 culture was devoid of any K10 staining ( Figure 4b).…”

Section: Resultsmentioning

confidence: 96%

The immortalizing and transforming ability of two common human papillomavirus 16 E6 variants with different prevalences in cervical cancer

et al. 2010

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Persistent infection with high-risk human papillomaviruses (HPVs), especially type 16 has been undeniably linked to cervical cancer. The Asian-American (AA) variant of HPV16 is more common in the Americas than the prototype in cervical cancer. The different prevalence is based on three amino acid changes within the E6 protein denoted Q14H/H78Y/L83V. To investigate the mechanism(s) behind this observation, both E6 proteins, in the presence of E7, were evaluated for their ability to extend the life span of and transform primary human foreskin keratinocytes (PHFKs). Longterm cell culture studies resulted in death at passage 9 of vector-transduced PHFKs (negative control), but survival of both E6 PHFKs to passage 65 (and beyond). Compared with E6/E7 PHFKs, AA/E7 PHFKs were significantly faster dividing, developed larger cells in monolayer cultures, showed double the epithelial thickness and expressed cytokeratin 10 when grown as organotypic raft cultures. Telomerase activation and p53 inactivation, two hallmarks of immortalization, were not significantly different between the two populations. Both were resistant to anoikis at later passages, but only AA/E7 PHFKs acquired the capacity for in vitro transformation. Proteomic analysis revealed markedly different protein patterns between E6/E7 and AA/E7, particularly with respect to key cellular metabolic enzymes. Our results provide new insights into the reasons underlying the greater prevalence of the AA variant in cervical cancer as evidenced by characteristics associated with higher oncogenic potential.

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Section: Resultsmentioning

confidence: 96%

The immortalizing and transforming ability of two common human papillomavirus 16 E6 variants with different prevalences in cervical cancer

et al. 2010

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“…Ki67 is a commonly used marker in proliferating cells, yet surprisingly little is known about its function. The Ki67 gene is located on the human chromosome 10 and mouse chromosome 7 (Schonk et al, 1989;Fonatsch et al, 1991). Two alternatively spliced mRNA species encode two isoforms of the protein: a larger isoform (359 kD) and a smaller isoform (320 kD) (Gerdes et al, 1983;Schluter et al, 1993).…”

Section: Implications Of Ki67 Presence Beyond Cell Birthmentioning

confidence: 99%

Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post‐mitotic newborn neurons

Pacal¹,

Bremner²

2012

Developmental Dynamics

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We investigated the self‐aggregation of 12 short ionic oligopeptides constituted by 4–7 amino acid residues to establish useful structure–property relationships that might be exploited in the biomedical field by using the concept of molecular Lego. We show that the critical aggregation concentration (CAC) of tetrapeptides decreases with increasing hydrophobicity of neutral residues. Additionally, the dependence of the CAC of isomeric oligopeptides on the distribution of amino acid residues confirms the high tendency to self‐organization of molecules with alternating ionic and neutral residues. Indeed, atomic force microscopy (AFM) images recorded on oligopeptide solutions above the CAC show the presence of either fibrillar or spherical aggregates depending on oligopeptide structure and concentration, steric hindrance, solution pH, and time. The potential of the investigated oligopeptides in tissue engineering applications is supported by their in vitro cytocompatibility. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 889–897, 2010

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“…MIB-1 is an antibody against Ki-67, an excellent cellular marker of proliferation. 27 However, no relationship was observed between Wnt9A and MIB-1 expression levels. Accordingly, Wnt9a is unlikely to affect cell proliferation.…”

Section: Discussionmentioning

confidence: 98%

Enhanced expression of Wnt9a in the flexor tenosynovium in idiopathic carpal tunnel syndrome

Yamanaka

Menuki

Zenke

et al. 2015

Journal Orthopaedic Research

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This study aimed to clarify the association between abnormal Wnt signaling and the cause of idiopathic carpal tunnel syndrome (ICTS) and whether an association exists between Wnt signaling and cell proliferation in the flexor tenosynovium. The subjects included nine patients with ICTS; the controls were nine patients with distal radius fractures without any symptoms of carpal tunnel syndrome. We extracted mRNA from the flexor tenosynovium and compared the expression levels of genes encoding 17 types of Wnt in both subjects and controls via quantitative real-time polymerase chain reaction (PCR). Expression levels of factors involved in cell proliferation, such as estrogen-responsive finger protein, epidermal growth factor receptor, heparin binding-epidermal growth factor-like growth factor, insulin-like growth factor-1, and vascular endothelial growth factor (VEGF) were also measured using quantitative real-time PCR. In addition, we compared the Wnt and MIB-1 protein expression levels to clarify the effect of Wnt on cell proliferation. Quantitative real-time PCR revealed significantly greater expression of the gene encoding Wnt9a in subjects with ICTS than in controls and also revealed a positive correlation between the expression of genes encoding Wnt9a and VEGF in subjects with ICTS. Quantitative evaluation using immunohistochemical staining also indicated more marked Wnt9a expression in subjects than in controls. However, there was no relationship between the expression of Wnt9a and the cell proliferation index MIB-1. These results indicate that Wnt9a expression is enhanced in ICTS and that Wnt9a may be involved in VEGF expression in ICTS. Keywords: idiopathic carpal tunnel syndrome; Wnt signal; vascular endothelial growth factor; MIB-1; flexor tensynovium Carpal tunnel syndrome (CTS) has the highest incidence among all entrapment syndromes, with an overall prevalence of approximately 3.8%. 1 The majority of CTS cases are idiopathic. 2 Although the exact pathological mechanism of idiopathic CTS (ICTS) remains unknown, some characteristic features of ICTS have been revealed. For example, ICTS is associated with fibroblast proliferation and collagen fiber overgrowth. Furthermore, median nerve compression and restricted range of motion consequent to these characteristics may be among the causative factors associated with ICTS. 3,4 In addition, few inflammatory changes have been observed in the flexor tenosynovium in ICTS 5 ; instead, blood vessel wall destruction and angiogenesis accompanied by a degree of edema may be detected. 3 Increased expression of cell proliferation factors, such as vascular endothelial growth factor (VEGF) and connective tissue growth factor (CTGF), in synovial cells is a feature of ICTS. 6,7 However, the mechanism by which the expression of these factors increases remains unknown.Wnt signaling controls initial cell development, morphogenesis, and organogenesis as well as postnatal cell proliferation, differentiation, and activity, and 19 types of Wnt ligands have been identifie...

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Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10

Cited by 97 publications

References 13 publications

The immortalizing and transforming ability of two common human papillomavirus 16 E6 variants with different prevalences in cervical cancer

The immortalizing and transforming ability of two common human papillomavirus 16 E6 variants with different prevalences in cervical cancer

Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post‐mitotic newborn neurons

Enhanced expression of Wnt9a in the flexor tenosynovium in idiopathic carpal tunnel syndrome

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