Assignment of the Gene for Porcine Insulin-Like Growth Factor Binding Protein 1 to Chromosome 18 and Detection of Polymorphisms in Intron 2 by PCR?RFLP

Inage-Miyake, Yuko; Shimanuki, S.; Itoh, Tomomichi; Murakami, Yasuto; Kimura, Masahiro; Suzuki, H.; Miyake, Masashi; Toki, Daisuke; Uenishi, Hirohide; Awata, Takashi; Hamasima, Noriyuki

doi:10.1007/s10528-005-1069-1

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“…To date, porcine IGFBP-1 (Inage-Miyake et al 2005), -3 (Lahbib et al 1996), -4 (Wang et al 2005, and -5 (Looft et al 2000) have been mapped on the porcine chromosome but not IGFBP-2. Also, no studies have been done on the association between the polymorphism and the production performance of this gene.…”

Section: Introductionmentioning

confidence: 99%

Chromosome Location and Association of Haplotypes of Insulin-like Growth Factor Binding Protein-2 with Production Performance in Swine

Wang

Meng

et al. 2008

Biochem Genet

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Insulin-like growth factor binding protein-2 is a member of the insulin-like growth factor families. Using a porcine RH panel, the gene was mapped on chromosome 15q22-23. Meanwhile, using polymerase chain reaction single strand conformation polymorphism, genotypic and allelic frequencies were analyzed in 17 pig breeds (total animals 570), together with a chi-square test of Hardy-Weinberg equilibrium. Also the association between haplotypes and production performance was analyzed in a Lantang x Landrace population family (n = 133, total 43 traits). At each locus we investigated, all the breeds showed different genotypic and allelic frequency distributions. In general, the Chinese native pig breeds carried a higher allele A frequency (over 50%) than the European pigs. For production performance, pigs with the CAG haplotype had higher fore-body and rear-body weight than those with the TGT and TAG haplotypes (P < 0.05). Also, pigs with the CAG haplotype had higher bone weight of the rear-body than those with the CAT haplotype (P < 0.05); pigs with the TGT and CAG haplotypes had higher forelimb and rearlimb weight than those with the CAT haplotype (P < 0.01 and P < 0.05, respectively); pigs with the TGG haplotype had higher leaf fat weight than those with the TGT and CAG haplotypes (P < 0.05); and pigs with the CAG haplotype had more stomach weight than those with the CAT and CGT haplotypes (P < 0.01); pigs with the TGT and CAG haplotypes had more ribs and longer body than those with the CGT-TGG, and CAT-TAG haplotypes (P < 0.05). These results suggest that IGFBP-2 is associated with production performance, but our population family was small. More studies with large samples are needed before the IGFBP-2 locus will be useful for a selection program.

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Section: Introductionmentioning

confidence: 99%