Assignment of Clostridium bryantii to Syntrophospora bryantii gen. nov., comb. nov. on the Basis of a 16S rRNA Sequence Analysis of Its Crotonate-Grown Pure Culture

Phylogeny of the Ammonia-Producing Ruminal Bacteria Peptostreptococcus anaerobius, Clostridium sticklandii, and Clostridium aminophilum sp. nov.

“…In this and other studies, analyses of 16s rRNA sequences of clostridia and related bacteria have shown that many of these bacteria are only distantly related (4,30,32). The genus Clostridium contains a highly diverse group of bacteria, and we agree with Cat0 and Stackebrandt (6) that the old description of these organisms as strictly anaerobic, sporeforming rods is clearly inadequate.…”

Phylogenetic Positions of Desulfofustis glycolicus gen. nov., sp. nov. and Syntrophobotulus glycolicus gen. nov., sp. nov., Two New Strict Anaerobes Growing with Glycolic Acid

“…(14), and Desulfobulbus sp. (6) represent a phylogenetic cluster as also indicated by similarity values of 90.6 and 86.5%, respectively ( Table 1 and Desulfotomaculum orientis (6) share 91.2% and 88.6% 16s (4,5,22,31,34). However, the significance of such a grouping is low, as indicated by the short internode distance of the branch containing the former organisms or groups and the Syntrophospora-Syntrophomonas (34) branch.…”

A Comparison of the Use of In Vitro–Transcribed and Native rRNA for the Quantification of Microorganisms in the Environment

“…It was later grown in pure culture on fumarate [32]. Syntrophomonas wolfeii LYB is a saturated fatty acid-beta-oxidizing bacterium capable of growing syntrophically with methanogens, or in pure culture on crotonate [36]. Native rRNA was extracted from pure cultures using a low-pH, hot-phenol, bead-beating method [28,30], with the following minor modifications: Phenol and phenol:chloroform solutions were buffered at pH 4.3, and nucleic acids were precipitated overnight at −20°C with one-half volume of 7.5 M ammonium acetate and two volumes of ethanol.…”

“…Since these organisms generally grow only in co-culture with syntrophic partners, quantifying relative population abundance (expressed as specific rRNA over total rRNA) is difficult, because nucleic acids extracted from cocultures contain rRNA from methanogens or sulfatereducing bacteria. Some of these syntrophic organisms can grow alone on alternative substrates such as fumarate and crotonate [31,36], or can grow slowly while reducing sulfate [34]. However, in practice, it often remains difficult to obtain adequate amounts of high quality rRNA from these organisms.…”