Assignment of Brevibacterium stationis (ZoBell and Upham 1944) Breed 1953 to the genus Corynebacterium, as Corynebacterium stationis comb. nov., and emended description of the genus Corynebacterium to include isolates that can alkalinize citrate

Bernard, Kathryn; Wiebe, Deborah; Burdz, Tamara; Reimer, Aleisha; Ng, Betty; Singh, Cathleen; Schindle, Samantha; Pacheco, Ana Luisa

doi:10.1099/ijs.0.012641-0

“…Menaquinones for C. macginleyi were not elucidated as part of the species novum description (14), and so this assay was undertaken for NML 080212, and it was analyzed by liquid chromatography-mass spectrometry (LC-MS), as described previously (4). Minor amounts of MK-8 (H2) were detected, which is consistent with results for the genus Corynebacterium (6).…”

Section: Case Reportsupporting

confidence: 62%

Corynebacterium macginleyi Conjunctivitis in Canada

Alsuwaidi

¹

,

Wiebe

²

,

Burdz

³

et al. 2010

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This report describes for the first time Corynebacterium macginleyi as a cause of conjunctivitis in Canada, where menaquinone analysis was done as part of the strain characterization. This species is typically isolated from ocular surfaces of patients from Europe and Japan. The isolate was resistant to erythromycin and clindamycin. CASE REPORTA 54-year-old microbiologist was seen by an ophthalmologist in Winnipeg, Manitoba, Canada, for evaluation of bilateral conjunctivitis. She had no history of eye injury or prior eye complaints, nor was a laboratory-acquired infection suspected. None of her family members had similar symptoms. She had no recent travel outside North America. Swabs from each eye were sent within several hours to a hospital laboratory for direct microscopy and bacterial culture. Until culture results were known, the patient was told to apply an over-the-counter "tear refresher" to the eyes to provide some relief. Approximately 5 days later, when microbiological results became apparent, the patient was prescribed 1 drop of 0.5% moxifloxacin hydrochloride in each eye 3 times per day for 7 days. The infection by that point had begun to resolve, but the patient did comply with the entire treatment course and since has had no recurrence. No follow-up culture was done to see if the organism had been eradicated from the eyes.At the hospital laboratory, after ϳ48 h of incubation under aerobic and facultatively anaerobic conditions at 35°C on 5% sheep blood agar, the culture from each sample grew fine (ϳ1-mm-diameter), round, and convex colonies that were slightly alpha-hemolytic. Growth in broth was enhanced by the addition of ϳ1% (vol/vol) sterile Tween 80, and so a lipophilic corynebacterium was suspected. Staining revealed Gram-positive coccobacilli in short chains and clusters. A few larger Gram-positive bacillary forms (thicker at one end) were also found interspersed among the coccobacilli. No other bacterial types were recovered. Isolates from each eye were deemed to be identical to each other, and both strains were catalase positive, oxidase negative, and facultatively anaerobic. The API Coryne system (bioMérieux, Montreal, Canada) was used for identification by the testing laboratory, generating API code 5100305 after a 24-h incubation, and the bacterium could be identified as Corynebacterium macginleyi with a 99.5% probability by that system.One strain was referred to the Canadian National Microbiology Laboratory (NML) for confirmation of identification (strain NML 080212). Because such isolates have not been previously described as being recovered in Canada, the strain was extensively characterized. Conventional carbohydrate broth sugars enhanced with ϳ1% serum (vol/vol) and other biochemical testing procedures were used as reviewed previously (2). This strain slowly fermented glucose, sucrose, and ribose, but in contrast to the results of Riegel et al. (14) also slowly fermented glycogen and maltose as well as mannose, glycerol, and fructose but not galactose, lactose, mannitol, raffinose, ...

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“…The authors did not explicitly describe that the C. gottingense type strain was non-motile, as expected for a Corynebacterium species [14], but being non-motile had been reported for the other study strains using either the hanging drop or tube methods. Interestingly, two Canadian isolates alkalinized citrate, a substrate not often tested for among Corynebacterium species and hitherto had only been rarely observed [18]. We found based on review of all study strains that glucose, ribose and maltose usually were utilized but occasionally found to be non-reactive.…”

mentioning

confidence: 87%

Corynebacterium godavarianum Jani et al. 2018 and Corynebacterium hadale Wei et al. 2018 are both later heterotypic synonyms of Corynebacterium gottingense Atasayar et al. 2017, proposal of an emended description of Corynebacterium gottingense Atasayar et al. 2017

Bernard

¹

,

Pacheco

²

,

Burdz

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Seven strains of an unidentifiable Corynebacterium species recovered from blood cultures, urine or cerebrospinal fluid over 26 years, closest to but differentiated from Corynebacterium imitans by 16S rRNA gene and partial rpoB gene sequencing, were studied. In November 2017, Atasayar et al. described a blood culture isolate as Corynebacterium gottingense sp. nov., which had >99 % similarity by 16S rRNA gene sequencing to the Canadian strains. In January 2018, Jani et al. described Corynebacterium godavarianum sp. nov., recovered from the Godavari River, India, which also had >99 % similarity by 16S/rpoB sequencing to the Canadian strains and C. gottingense. In May 2018, Wei et al. described Corynebacterium hadale recovered from hadopelagic water; this too had >99 % similarity by 16S rRNA gene sequencing to C. gottingense , C. godavarianum and the Canadian strains. C. gottingense DSM 103494T and C. godavarianum LMG 29598T were acquired and whole genome sequencing was performed (not previously done). Results were compared with genomes from C. hadale (GenBank accession NQMQ01) and the Canadian isolates. We found that these ten genomes formed a single taxon when compared using digital DNA–DNAhybridization, average nucleotide identity using blastn and average amino acid identity criteria but exhibited some subtle biochemical and chemotaxonomic differences. Heuristically, we propose that C. godavarianum and C. hadale are later heterotypic synonyms of, and the Canadian isolates are identifiable as, C. gottingense . We provide an emended description of Corynebacterium gottingense Atasayar et al. 2017; genomes ranged from 2.48 to 2.69 Mb ( C. gottingense DSM 103494T, 2.62 Mb) with G+C content of 65.1–65.6 mol% (WGS), recovered from clinical and environmental sites.

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“…Eleven different species were isolated from the nasal cavities of the pigs. The most prevalent species was Corynebacterium xerosis , which was found in all but one of the pigs, followed by Brevibacterium stationis , which was recently proposed to belong to the Corynebacterium genus (Bernard and others 2010) and Corynebacterium confusum . The other eight species were only prevalent in less than 5 per cent of the animals.…”

Section: Resultsmentioning

confidence: 99%

“… * Brevibacterium stationis has recently proposed to belong to the Corynebacterium genus (Bernard and others 2010) …”

Section: Resultsmentioning

confidence: 99%

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Corynebacterium species nasal carriage in pigs and their farmers in Bavaria, Germany: implications for public health

Boschert

¹

,

Berger²,

Konrad³

et al. 2014

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Reports on cases of human diphtheria caused by toxigenic Corynebacterium ulcerans that were linked to occupational swine contact as well as isolation of C ulcerans from wild boars have suggested that pigs might serve as reservoir for human infections. Therefore, a prevalence study on Corynebacterium species nasal carriage in pigs and their farmers was performed between August 1 and December 31, 2009, in 41 swine farms from Bavaria, Germany. All 411 asymptomatic pigs and 29 of 30 healthy farmers were colonised with Corynebacterium strains of up to 11 different species. No potentially toxigenic Corynebacterium strain was isolated either from the pigs or from their farmers, respectively. The patterns of the species composition in the pigs and the farmers were very similar, suggesting a potential transmission of strains between animals and humans.

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Assignment of Brevibacterium stationis (ZoBell and Upham 1944) Breed 1953 to the genus Corynebacterium, as Corynebacterium stationis comb. nov., and emended description of the genus Corynebacterium to include isolates that can alkalinize citrate

Abstract: Assignment of

Cited by 58 publications

References 15 publications

Corynebacterium macginleyi Conjunctivitis in Canada

Corynebacterium macginleyi Conjunctivitis in Canada

Corynebacterium godavarianum Jani et al. 2018 and Corynebacterium hadale Wei et al. 2018 are both later heterotypic synonyms of Corynebacterium gottingense Atasayar et al. 2017, proposal of an emended description of Corynebacterium gottingense Atasayar et al. 2017

Corynebacterium species nasal carriage in pigs and their farmers in Bavaria, Germany: implications for public health

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