Assignment of 60 human ESTs in cattle

Laurent, Pascal; Elduque, C.; Hayes, Hélène; Saunier, Katiana; Eggen, Andre A.; Levéziel, Hubert

doi:10.1007/s003350010159

Cited by 22 publications

(19 citation statements)

References 17 publications

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“…These BAC clones could also serve as chromosome markers in other cytogenetic studies which require to trace a specific chromosome, for example X and Y [6], and the specific primers developed here could serve as an efficient tool to calibrate different existing hybrid somatic panels [1,10,11,17].…”

Section: Discussionmentioning

confidence: 99%

“…The isolated BAC is currently being studied to confirm the presence of the VIL1 gene and to describe a specific coding sequence. For BTA29, as LDHA could not be assigned to the somatic cell hybrid panel despite the fact that homologous primers were chosen [11], we proposed to solve the difficulties in assignment by choosing another marker gene for BTA29, IGF2. Both LDHA and IGF2 have been localised by radioactive ISH at the same telomeric end of BTA29 [15] and IGF2 has been mapped to BTA29 using the INRA somatic hybrid cell panel [11].…”

Section: Discussionmentioning

confidence: 99%

“…A more complete description of the panel is given in Laurent et al [11]. A correlation coefficient of 0.69 was used as the threshold for confident assignment of a marker to a chromosome [3].…”

Section: Chromosomal Assignment Using the Inra Hamster-bovine Somaticmentioning

confidence: 99%

“…A correlation coefficient of 0.69 was used as the threshold for confident assignment of a marker to a chromosome [3]. PCR-based assignments were performed according to Laurent et al [11].…”

Section: Chromosomal Assignment Using the Inra Hamster-bovine Somaticmentioning

confidence: 99%

“…In this work, PCR systems were developed from already published homologous or heterologous sequences for each of the marker genes and were used to assign the corresponding genes by analysis on the INRA hamster-bovine somatic cell hybrid panel [11] and to screen a bovine BAC library to obtain corresponding BAC clones.…”

Section: Introductionmentioning

confidence: 99%

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Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes

et al. 2001

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-In 1996, Popescu et al. published the Texas standard nomenclature of the bovine karyotype in which 31 marker genes, already mapped in man, were chosen to permit unambiguous identification and numbering of each bovine chromosome. However, specific PCR systems were not available for each marker gene thus preventing the assignment of part of these markers by somatic cell hybrid analysis. In addition, some difficulties remained with the nomenclature of BTA25, BTA27 and BTA29. In this work, specific PCR systems were developed for each of the marker genes except VIL1 (see results), from either existing bovine or human sequences, and a bovine BAC library was screened to obtain the corresponding BAC clones. These PCR systems were used successfully to confirm the assignment of each marker gene (except for LDHA, see results) by analysis on the INRA hamster-bovine somatic cell hybrid panel. The difficulties observed for LDHA and VIL1 are probably due to the fact that these genes belong to large gene families and therefore suggest that they may not be the most appropriate markers for a standardisation effort. This panel of BACs is available to the scientific community and has served as a basis for the establishment of a revised standard nomenclature of bovine chromosomes. bovine / BAC library / cytogenetics / mapping / Texas standard

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Chromosomal Assignment Using the Inra Hamster-bovine Somaticmentioning

confidence: 99%

“…A correlation coefficient of 0.69 was used as the threshold for confident assignment of a marker to a chromosome [3]. PCR-based assignments were performed according to Laurent et al [11].…”

Section: Chromosomal Assignment Using the Inra Hamster-bovine Somaticmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes

et al. 2001

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A 4 Mb high resolution BAC contig on bovine chromosome 1q12 and comparative analysis with human chromosome 21q22

et al. 2005

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The bovine RPCI-42 BAC library was screened to construct a sequence-ready ~4 Mb single contig of 92 BAC clones on BTA 1q12. The contig covers the region between the genes KRTAP8P1 and CLIC6. This genomic segment in cattle is of special interest as it contains the dominant gene responsible for the hornless or polled phenotype in cattle. The construction of the BAC contig was initiated by screening the bovine BAC library with heterologous cDNA probes derived from 12 human genes of the syntenic region on HSA 21q22. Contig building was facilitated by BAC end sequencing and chromosome walking. During the construction of the contig, 165 BAC end sequences and 109 single-copy STS markers were generated. For comparative mapping of 25 HSA 21q22 genes, genomic PCR primers were designed from bovine EST sequences and the gene-associated STSs mapped on the contig. Furthermore, bovine BAC end sequence comparisons against the human genome sequence revealed significant matches to HSA 21q22 and allowed the in silico mapping of two new genes in cattle. In total, 31 orthologues of human genes located on HSA 21q22 were directly mapped within the bovine BAC contig, of which 16 genes have been cloned and mapped for the first time in cattle. In contrast to the existing comparative bovine–human RH maps of this region, these results provide a better alignment and reveal a completely conserved gene order in this 4 Mb segment between cattle, human and mouse. The mapping of known polled linked BTA 1q12 microsatellite markers allowed the integration of the physical contig map with existing linkage maps of this region and also determined the exact order of these markers for the first time. Our physical map and transcript map may be useful for positional cloning of the putative polled gene in cattle. The nucleotide sequence data reported in this paper have been submitted to EMBL and have been assigned Accession Numbers AJ698510–AJ698674.

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Sex determination in cattle based on simultaneous amplification of a new male‐specific DNA sequence and an autosomal locus using the same primers

Weikard

Kühn

Brunner

et al. 2001

Molecular Reproduction Devel

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A PCR-based method for sex determination of bovine DNA samples and embryo biopsies is presented. Using only one primer pair both the male-specific sequence FBNY (127 bp) and a sex-independent control PCR-fragment, the microsatellite marker FBN17 (136-140 bp) are generated in the same PCR reaction. Synteny mapping assigned the male-specific sequence to bovine chromosome Y (BTA Y), whereas FBN17 was mapped to bovine chromosome 2. Localisation of FBNY on BTA Y was confirmed by fluorescence in hybridisation of two BAC clones containing the male-specific sequence. There was no amplification of the male-specific target sequence FBNY in sheep, pig, goat, mice, man, and several wild species of the tribe Bovini. The bovine male-specific fragment was detected in dilutions containing as little as 10 pg genomic DNA and in blastomeres from embryo biopsies. The PCR assay presented here does require neither restriction endonuclease digestion of the PCR product nor additional nested PCR steps. Owing to the advantage of parallel amplification of the autosomal locus FBN17 no additional control fragment is necessary to detect PCR failure. The results of sex determination in embryo biopsies using FBNY were in agreement with the outcome from a reference assay used in commercial breeding programs.

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Assignment of 60 human ESTs in cattle

Cited by 22 publications

References 17 publications

Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes

Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes

A 4 Mb high resolution BAC contig on bovine chromosome 1q12 and comparative analysis with human chromosome 21q22

Sex determination in cattle based on simultaneous amplification of a new male‐specific DNA sequence and an autosomal locus using the same primers

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