Assessments of the effects of nicotine and ketamine using tyrosine hydroxylase-green fluorescent protein transgenic zebrafish asbiosensors

Suen, Miranda F.K.; Chan, W.S.; Hung, Karen W.Y.; Chen, Y.F.; Mo, Zhixian; Yung, Kin Lam

doi:10.1016/j.bios.2012.09.042

Cited by 25 publications

(16 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are almost no published studies on the effects of the main classes of hallucinogenic drugs on circling behavior in larval zebrafish, necessitating future testing. The only study screening ketamine effects in larval zebrafish, 158 however, did report aberrant circling-like (looplike) swimming, further paralleling adult fish findings discussed above. Given the similarity of observed drug-induced "circling" behavior across various species (see ref 70 for discussion), zebrafish appear to mimic specific neurological phenotypes evoked by selected hallucinogenic drugs in various nonfish studies.…”

Section: ■ Modeling Hallucinogenic Drug Action In Zebrafishsupporting

confidence: 63%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

View full text Add to dashboard Cite

Among different classes of psychotropic drugs, hallucinogenic agents exert one of the most prominent effects on human and animal behaviors, markedly altering sensory, motor, affective, and cognitive responses. The growing clinical and preclinical interest in psychedelic, dissociative, and deliriant hallucinogens necessitates novel translational, sensitive, and high-throughput in vivo models and screens. Primate and rodent models have been traditionally used to study cellular mechanisms and neural circuits of hallucinogenic drugs' action. The utility of zebrafish (Danio rerio) in neuroscience research is rapidly growing due to their high physiological and genetic homology to humans, ease of genetic manipulation, robust behaviors, and cost effectiveness. Possessing a fully characterized genome, both adult and larval zebrafish are currently widely used for in vivo screening of various psychotropic compounds, including hallucinogens and related drugs. Recognizing the growing importance of hallucinogens in biological psychiatry, here we discuss hallucinogenic-induced phenotypes in zebrafish and evaluate their potential as efficient preclinical models of drug-induced states in humans.

show abstract

Section: ■ Modeling Hallucinogenic Drug Action In Zebrafishsupporting

confidence: 63%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Both rat anti-DAT primary antibody (Chemicon, Temecula, CA) and rabbit polyclonal anti-TH (Chemicon) have been verified to be reactive with zebrafish [19,20]. The blots were washed five times for 10 min each with tris-buffered saline Tween-20 (TBST) and then incubated with goat anti-rat (1:5,000) or anti-rabbit…”

Section: Protein Extraction and Western Blot Analysismentioning

confidence: 99%

The developmental neurotoxicity of polybrominated diphenyl ethers: Effect of DE‐71 on dopamine in zebrafish larvae

Wang

Yang

et al. 2015

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Abstract:The potential neurotoxicity of polybrominated diphenyl ethers (PBDEs) is still a great concern. In the present study, we investigated whether exposure to PBDEs could affect the neurotransmitter system and cause developmental neurotoxicity in zebrafish. Two hours post fertilization (hpf) zebrafish embryos were exposed to different concentrations of the PBDE mixture DE-71 (0-100 µg/L). The larvae were harvested at 120 hpf and the impact on dopaminergic signaling was investigated. The results revealed significant reductions of whole-body dopamine (DA) content and its metabolite, dihydroxyphenylacetic acid (DOPAC) content in DE-71-exposed larvae. The transcription of genes involved in the development of dopaminergic neurons (e.g. manf, bdnf, and nr4a2b) was significantly down-regulated upon exposure to DE-71. DE-71 also resulted in a significant decrease of tyrosine hydroxylase (TH) and dopamine transporter protein levels in dopaminergic neurons. The expression level of TH in forebrain neurons was assessed by whole-mount immunofluorescence, and the results further demonstrated that the TH protein expression level was reduced in dopaminergic neurons. In addition to these molecular changes, we also observed reduced locomotor activity in DE-71-exposed larvae. Taken together, the present study demonstrates that acute exposure to PBDEs can affect dopaminergic signaling through disrupting the synthesis and transportation of dopamine in zebrafish, thereby disrupting normal neurodevelopment.In accord with its experimental findings, this study extends our knowledge of the mechanisms governing PBDE-induced developmental neurotoxicity. This article is protected by copyright. All rights reserved

show abstract

“…Besides, they also implied that the agitation might be positively correlated to dopamine release in central nervous system. [19]. Thereby we can assume that it should be a complicated process after ketamine exposure, which is consisted of multiple nervous circuits behind different behavior phenotypes.…”

Section: Functional Damage Of Ketamine In Zebrafish's Nervous Systemmentioning

confidence: 99%

“…Given that we utilize the transgenic zebrafish as live biosensors to test the side effects of ketamine, whose specific regions can be labeled by some visible proteins (GFP) [17][18][19][20]. Secondly, short mature period and generous reproduction make it more suitable for high-throughput screening than mice [21].…”

Section: Introductionmentioning

confidence: 99%

Zebrafish as a New Platform Used in Exploration of Ketamine-Induced Neurodevelopmental Toxicity

Ruan¹

2015

J Clin Exp Pathol

View full text Add to dashboard Cite

We have modified the abstract as 'Previous studies have already found out that commonly used anesthetic, ketamine, has toxic effects on neurodevelopment. Unlike most rodent models just focusing on neuronal apoptosis caused by ketamine, early stages of neuron development abnormality in zebrafish can be assessed in vivo because of the transparent embryos and larve. And also thanks to its cost-efficiency and quick reproduction, large-scale behavior analyses and gene screens can be conducted in zebrafish. Besides, the whole genome of zebrafish has already been sequenced and its gene functions are highly conserved during evolution, which makes the experiments more reliable on zebrafish model. So Zebrafish has obvious advantages in the researches of ketamine neurotoxicity over the conventional animal models (such as mice). Within this paper we illuminate how we can use this model to study ketamine neurotoxicity. In the future, along with more advanced genetic technologies joining this platform will not only make up for conventional models to deeply understand neurodevelopmental toxicity of ketamine, but also might provide the unique insight to the field of neurodevelopment and neurotoxicity impaired by other drugs

show abstract

Assessments of the effects of nicotine and ketamine using tyrosine hydroxylase-green fluorescent protein transgenic zebrafish asbiosensors

Cited by 25 publications

References 63 publications

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

The developmental neurotoxicity of polybrominated diphenyl ethers: Effect of DE‐71 on dopamine in zebrafish larvae

Zebrafish as a New Platform Used in Exploration of Ketamine-Induced Neurodevelopmental Toxicity

Contact Info

Product

Resources

About