Assessments for bone regeneration using the polycaprolactone SLUP (salt‐leaching using powder) scaffold

Abstract

Salt-leaching using powder (SLUP) scaffolds are novel salt-leaching scaffolds with well-interconnected pores that do not require an organic solvent or high pressure. In this study, in vitro and in vivo cell behaviors were assessed using a PCL (polycaprolactone) SLUP scaffold. Moreover, using PCL, conventional salt-leaching and 3D-plotted scaffolds were fabricated as control scaffolds. Morphology, mechanical property, water absorption, and in vitro/in vivo cell response assessments were performed to clarify the characteristics of the SLUP scaffold compared with control scaffolds. Consequently, we verified that the interconnectivity between the pores of the SLUP scaffold was enhanced compared with conventional salt-leaching scaffolds. Moreover, in vitro cell attachment and proliferation of the SLUP scaffold were higher than those of the 3D-plotted scaffold because of their morphological characteristic. Furthermore, we revealed that new bone formation and bone ingrowth of the SLUP scaffold was superior to those of the calvarial defect model and 3D-plotted scaffold because of the high porosity and improved interconnectivity of pores by the SLUP technique without high pressure using powders. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3432-3444, 2017.

PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry

“…Several techniques have been developed to produce polymeric scaffolds for tissue engineering, including electrospinning technique [30], 3D printing [31], salt leaching [32], and thermally induced phase separation (TIPS) [33,34]. Hybrid Poly-e-caprolactone-polyglycolic acid solution was used to produce biodegradable scaffolds by a 3D mold for human tooth-ligament in dental applications [35].…”

Highly porous polycaprolactone scaffolds doped with calcium silicate and dicalcium phosphate dihydrate designed for bone regeneration

“…Several techniques have been developed to produce scaffolds for tissue engineering, including electrospinning technique [12], 3D printing [18], salt leaching [19] and thermally induced phase separation (TIPS) [9,20]. TIPS procedure allows the preparation of highly porous (>90%) scaffolds without the need of high temperatures that may alter the properties of the polymer matrix or those of the mineral filler [9].…”

Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine

“…PCL pellets were melted in a heating cylinder at 100-130°C, and 3D-printed scaffolds were fabricated using a Bioplotter (Envisiontec GmbH, Gladbeck, Germany) [32]. PCL was extruded through a heated nozzle in the form of a strand, which was plotted on a plate in a layer-by-layer deposition manner [32][33][34]. The samples had a nominal ber diameter of 300 µm (based on the 27 G nozzle size), the layer thickness of 280-300 µm, and a ber spacing of 700 µm.…”